Investigation of Extraction-Type-Dependent Changes in Antioxidant Capacity of Euphorbia peplus L.

Yıl 2026, Cilt: 6 Sayı: 1, 21 - 25, 11.03.2026

Gülşen Güçlü

https://doi.org/10.59359/maujan.1846297

https://izlik.org/JA97JB32NB

Öz

Euphorbia peplus L. is a species found in North Africa, Europe, and Western Asia, characterized by significant toxicity capasity and a rich phytochemical content including sterols, flavonoids, and diterpenes. These compounds provide the plant with anti-inflammatory, antimicrobial, anticancer, and allelopathic properties. To expand the limited studies on its antioxidant capacity, this study comparatively analyzed the in vitro antioxidant capacity of E. peplus L. extracts prepared with three different solvents (water, 80% ethanol, methanol). According to the radical scavenging activity results obtained by the DPPH method, the water extract (517.5 ± 0.7 µg/mL) showed higher antioxidant activity than the ethanol (1165 ± 1.2 µg/mL) and methanol (1275 ± 1.4 µg/mL) extracts. When TPC and TFC contents were examined using the Folin–Ciocalteu and colorimetric methods, the highest TPC (158.2 ± 0.07 mg GAE/g) and TFC (14.03 ± 0.02 mg RE/g) values were determined in the ethanol extract. The antioxidant activity, total phenolic content, and flavonoid content of E. peplus L. water extract have been reported for the first time, and it is thought that this study could provide a potential source for solvent selection in determining the active components of antioxidants in future studies of the plant.

Anahtar Kelimeler

Euphorbia peplus L. , Antioxidant activity , Extract , TPC , TFC

Euphorbia peplus L.'nin antioksidan kapasitesinde ekstraksiyon tipine bağlı değişikliklerin araştırılması

https://izlik.org/JA97JB32NB

Öz

Euphorbia peplus L., Kuzey Afrika, Avrupa ve Batı Asya'da bulunan, önemli toksisite özellik gösteren ve steroller, flavonoidler ve diterpenler dahil olmak üzere zengin fitokimyasal içeriği ile karakterize edilen bir türdür. Bu bileşikler, bitkiye antiinflamatuar, antimikrobiyal, antikanser ve allelopatik özellikler kazandırır. Bitkinin antioksidan kapasitesi üzerine yapılan sınırlı çalışmaları genişletmek amacıyla, bu çalışmada üç farklı çözücü (su, %80 etanol, metanol) ile hazırlanan E. peplus L. özütlerinin in vitro antioksidan kapasitesi karşılaştırmalı olarak analiz edilmiştir. DPPH yöntemi ile elde edilen radikal temizleme aktivitesi sonuçlarına göre, su ekstresi (517,5 ± 0,7 µg/mL) etanol (1165 ± 1,2 µg/mL) ve metanol (1275 ± 1,4 µg/mL) ekstrelerine göre daha yüksek antioksidan aktivite göstermiştir. Folin–Ciocalteu ve kolorimetrik yöntemler kullanılarak TPC ve TFC içerikleri incelendiğinde, en yüksek TPC (158,2 ± 0,07 mg GAE/g) ve TFC (14,03 ± 0,02 mg RE/g) değerleri etanol ekstraktında belirlenmiştir. E. peplus L. su ekstraktının antioksidan aktivitesi, toplam fenolik içeriği ve flavonoid içeriği ilk kez rapor edilmiştir ve bu çalışmanın, bitkinin gelecekteki çalışmalarında antioksidanların aktif bileşenlerinin belirlenmesinde çözücü seçimi için potansiyel bir kaynak sağlayabileceği düşünülmektedir.

Anahtar Kelimeler

Euphorbia peplus L. , Antioksidan aktivite , Ekstrakt , TPC , TFC

Kaynakça

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