Effect of Electroporation on Antiproliferative Activity of Rheum Ribes and Hypericum Perforatum Extracts in Lung Cancer Cells

Yıl 2025, Cilt: 5 Sayı: 3, 1 - 12, 29.12.2025

Mehmet Eşref Alkış , Fuat Yetişsin

https://doi.org/10.71255/maunsbd.1634531

https://izlik.org/JA67UH42FN

Öz

The purpose of this study is to investigate the phenolic substance contents of Hypericum perforatum and Rheum ribes plants and their cytotoxicity on lung cancer cells (A549) and to reveal the effects of electroporation (EP) on the antiproliferative activities of these plants. Antiproliferative activities of plant extracts were determined in A549 cells, and their biocompatibility was evaluated in L-929 fibroblast cells through MTT assay. In electrochemotherapy (extracts+EP) applications of A549 cancer cells, eight square wave electrical pulse sequences with an intensity of 800V/cm were used with various doses of plant extracts. Both plant extracts were observed to be abundant in phenolic compounds and exhibited almost no cytotoxic effects, with IC50 values higher than 1000µg/mL for L-929 fibroblast cells. However, A549 cancer cells showed very good sensitivity to the cytotoxic activities of Rheum ribes and Hypericum perforatum extracts, with IC50 values of 297.32 and 241.10 µg/mL, respectively. The cytotoxic activity of both plant extracts increased considerably with EP, and cell viability percentages of extract+EP groups were observed to decrease significantly compared to extract-only groups (p<0.05). Our findings reveal that Rheum ribes and Hypericum perforatum extracts have anticancer potential and may be promising for lung cancer if used in combination with EP.

Anahtar Kelimeler

Hypericum perforatum , Rheum ribes , Lung cancer , cytotoxicity , Electroporation

Elektroporasyonun Akciğer Kanseri Hücrelerinde Rheum Ribes ve Hypericum Perforatum Ekstratlarının Antiproliferatif Aktivitesi Üzerine Etkisi

https://izlik.org/JA67UH42FN

Öz

Bu çalışmanın amacı, Hypericum perforatum ve Rheum ribes bitkilerinin fenolik madde içeriklerini ve akciğer kanseri hücreleri (A549) üzerindeki sitotoksisitelerini belirlemek ve elektroporasyonun (EP) bu bitkilerin antiproliferatif aktiviteleri üzerindeki etkilerini ortaya koymaktır. Bitki ekstraktlarının antiproliferatif aktiviteleri A549 hücrelerinde, biyouyumlulukları ise L-929 fibroblast hücrelerinde MTT analizi ile test edilmiştir. A549 kanser hücrelerinin elektrokemoterapi (ekstraktlar+EP) uygulamalarında, 800V/cm yoğunluğunda sekiz kare dalga elektriksel puls dizisi, bitki ekstraktlarının çeşitli dozları ile birlikte kullanılmıştır. Her iki bitki ekstraktının da fenolik bileşikler bakımından zengin olduğu ve L-929 fibroblast hücreleri için 1000µg/mL'den daha yüksek IC50 değerleri ile neredeyse hiç sitotoksik etki göstermediği bulunmuştur. Bununla birlikte, A549 kanser hücreleri Rheum ribes ve Hypericum perforatum ekstraktlarının sitotoksik aktivitelerine karşı çok iyi duyarlılık göstermiş ve IC50 değerleri sırasıyla 297,32 ve 241,10 µg/mL olarak bulunmuştur. Her iki bitki ekstraktının sitotoksik aktivitesi EP ile önemli ölçüde artmış ve ekstrakt+EP gruplarının hücre canlılık yüzdelerinin sadece ekstrakt gruplarına kıyasla önemli ölçüde azaldığı görülmüştür (p<0.05). Bulgularımız, Rheum ribes ve Hypericum perforatum ekstraktlarının antikanser potansiyeline sahip olduğunu ve EP ile birlikte kullanıldığında akciğer kanseri için umut verici olabileceğini ortaya koymaktadır.

Anahtar Kelimeler

Hypericum perforatum , Rheum ribes , Akciğer kanseri , Sitotoksisite , Elektroporasyon

Kaynakça

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