İnsan, Koyun ve Sığır Kistik Ekinokokkozis İzolatlarının NAD1 Gen Bölgesinin Moleküler Karakterizasyonu

Öz

Bu çalışmanın amacı, insan ve hayvan sağlığı açısından önemli bir paraziter etken olan Echinococcus granulosus’un neden olduğu kistik ekinokokkozisle mücadeleye katkı sağlamak amacıyla, farklı konaklardan elde edilen örneklerde baskın suşların moleküler yöntemlerle belirlenmesiydi. Bu çalışma kapsamında, insanlardan 75 kist materyali, sığırdan 25 kist materyali ve koyundan 25 kist materyali kullanıldı. Kist sıvılarının mikroskobik incelemesinin ardından, kist zarı/sıvısından DNA izole edildi ve PCR ile NAD1 mitokondriyal gen bölgesi çoğaltıldı. 60 kist örneği, PCR amplikon dizi analiziyle incelendi ve E. granulosus izolatlarının suşları belirlendi. Ayrıca, NAD1 mitokondriyal gen bölgesinde mutasyonlar tespit edildi. Koyun ve sığırda hidatik kist organ lokalizasyonunda, sığırlarda en yaygın lokalizasyonun karaciğer, koyunlarda ise en yaygın lokalizasyonun akciğer olduğu görüldü. Dizi analizi sonuçları NCBI’deki referans suşlarla karşılaştırıldığında, dokuz örneğin G3 (%15), kalan 51 (%85) örneğin ise G1 suşu olduğu tespit edildi. Bu çalışmada ilk defa dokuz örnekte mutasyon olduğu belirlendi. Van bölgesinde G1 (koyun) suşunun yaygınlığı, koyunların bölgede E. granulosus’un yayılımında önemli bir rol oynadığını göstermektedir.

Anahtar Kelimeler

• cystic echinococcosis
• NAD1
• KOYUN
• SIĞIR

Proje Numarası

TDK-2016-5407

Host-Based Analysis of Genetic Diversity in Echinococcus granulosus Isolates Originating from Van Province

Öz

The aim of this study was to determine the dominant strains from samples of different hosts using molecular methods to contribute to the fight against cystic echinococcosis caused by Echinococcus granulosus, which is an important parasitic agent for human and animal health. Within the scope of this study, 75 cyst materials from humans, 25 cyst materials from cattle, and 25 cyst materials from sheep were used. After microscopic examination of cyst fluids, DNA was isolated from the cyst membrane/fluid and the NAD1 mitochondrial gene region was amplified by PCR. 60 cyst samples were analyzed by PCR amplicon sequence analysis and the strains of E. granulosus isolates were identified. Mutations in the NAD1 mitochondrial gene region were also identified. Hydatid cyst organ localization in sheep and cattle showed that liver localization was the most common in cattle while lung localization was the most common in sheep. When the results of sequence analysis were compared with the reference strains in NCBI, nine samples were found to be G3 (%15), and the remaining 51 (%85) samples were G1 strains. In this study, we determined, for the first time, that nine samples had mutations. The prevalence of G1 (sheep) strain in Van region indicates that sheep play an important role in the prevalence of E. granulosus in the region.

Anahtar Kelimeler

• cattle
• cystic echinococcosis
• human
• genotyping
• NAD1
• sheep

Destekleyen Kurum

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Proje Numarası

TDK-2016-5407

Etik Beyan

Van Yüzüncü Yıl Üniversitesi Girişimsel Olmayan Klinik Araştırmalar Etik Kurulu

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