The protective effect of venlafaxine on hydrogen peroxide-induced cytotoxicity in C6 glioma cells

Yıl 2024, , 77 - 86, 30.04.2024

Adem Ahlatcı , Kenan Yıldızhan , Ahmet Şevki Taşkıran

https://doi.org/10.30565/medalanya.1459206

Öz

Aim: Neurodegeneration is the progressive loss and structural deterioration of neuronal cells. Hydrogen peroxide (H2O2) is formed by dismutation and causes oxidative stress in neuronal cells. Venlafaxine is a drug that increases both serotonin and noradrenaline in the synaptic gap.In this study, the effect of venlafaxine on H2O2-induced cytotoxicity in C6 cells was investigated.

Methods: First of all, different doses of venlafaxine (25, 50, and 100 µM) were tried to find the appropriate dose in C6 glioma cells. Then, the effect of venlafaxine on H2O2-induced cytotoxicity in the cells was investigated. For this purpose, cell viability rate, proinflammatory markers IL-1β and TNF-α, and NO and iNOS levels were examined by ELISA kits.

Results: H2O2-treated caused cytotoxicity in the C6 glioma cells; when venlafaxine 25, 50, and 100 μM doses were evaluated in terms of cell viability, it was observed that the 100 μM venlafaxine applied group significantly increased cell viability compared to the other groups. When we look at the levels of IL-1β and TNF-α, it is observed that there is an increase in the H2O2 applied group and a significant decrease in the venlafaxine (100 μM) applied group. It was observed that NO and iNOS levels increased in the H2O2 applied group compared to the other groups. It was observed that Venlafaxine treatment reduced the increased NO and iNOS levels caused by H2O2.

Conclusion: The study results showed that venlafaxine may have a protective effect on H2O2-induced cytotoxicity in C6 glioma cells.

Anahtar Kelimeler

Cytotoxicity, C6 cells, Venlafaxine, Hydrogen Peroxide

Venlafaksi’nin C6 Glioma Hücrelerinde Hidrojen Peroksit Kaynaklı Sitotoksisitede Koruyucu Etkisi

Öz

Amaç: Nörodejenerasyon, nöron hücrelerinin ilerleyici kaybı ve yapısal bozulmasıdır. Hidrojen peroksit (H2O2) dismutasyonla oluşur ve nöron hücrelerinde oksidatif strese neden olur. Venlafaksin, sinaptik boşlukta hem serotonini hem de noradrenalini artıran bir ilaçtır.Bu çalışmada venlafaksi’nin C6 hücrelerinde H2O2 kaynaklı sitotoksisite üzerindeki etkisini araştırıldı.

Yöntem: Öncelikle C6 glioma hücrelerinde uygun dozu bulmak için farklı dozlarda venlafaksin (25, 50 ve 100 µM) denendi. Daha sonra venlafaksi’nin hücrelerde H2O2 kaynaklı sitotoksisite üzerine etkisi araştırıldı. Bu amaçla hücre canlılık oranı, IL-1β, TNF-α, NO ve iNOS düzeyleri ELISA kitleri ile incelendi.

Bulgular: H2O2 ile inkübasyon C6 glioma hücrelerinde sitotoksisiteye neden oldu. Venlafaksin 25, 50 ve 100 μM dozları hücre canlılığı açısından değerlendirildiğinde, 100 μM venlafaksin uygulanan grubun diğer gruplara göre hücre canlılığını anlamlı düzeyde arttırdığı görüldü. IL-1β ve TNF-α düzeylerine bakıldığında H2O2 uygulanan grupta artış, venlafaksin (100 μM) uygulanan grupta ise IL-1β ve TNF-α düzeylerinde anlamlı oranda azalma olduğu görüldü. H2O2 uygulanan grupta NO ve iNOS düzeylerinin diğer gruplara göre arttığı gözlendi. Venlafaksin tedavisinin H2O2'nin neden olduğu artan NO ve iNOS düzeylerini azalttığı görüldü.

Sonuç: Çalışma sonuçları venlafaksinin C6 glioma hücrelerinde H2O2 kaynaklı sitotoksisite üzerinde koruyucu etkiye sahip olabileceğini gösterdi.

Anahtar Kelimeler

Sitotoksisite, C6 hücreleri, Venlafaksin, Hidrojen peroksit

Kaynakça

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