The Protective Effects Of Beta Glucan Against Experimental Renal Ischemia Reperfusion Injury

Yıl 2022, , 80 - 86, 27.03.2022

Ayşegül Mavi Bulut , Ferhat Şirinyıldız , Cenk Orak , Gökhan Cesur

https://doi.org/10.30565/medalanya.996861

Öz

Aim: This study aims to investigate the possible protective effects of beta-glucan against oxidative stress caused by ischemia and reperfusion injury in kidney tissue.

Methods: In the study, 30 male Wistar albino rats weighing 300-350g were used (n=10). Rats were randomly grouped into three groups of Sham control, ischemia reperfusion group (IR), ischemia reperfusion + beta glucan group (IR + BG). Sham group had left nephrectomy, the right kidney taken for histopathologic and biochemical examination. After left nephrectomy in IR group, ischemia procedure was applied for 45 minutes via nontraumatic microvascular clamp, then reperfusion was applied for 60 minutes in the right kidney. In the IR+BG group, rats were administered 100 mg/kg beta glucan via gastric gavage for 10 days. Reperfusion was applied for 60 minutes after 45 minutes of ischemia to the right kidney under anesthesia.

Results: As a result of biochemical examination MDA values showed a significant increase in IR group compared to Sham group (p<0,05). In IR+BG group, there was a significant decrease compared to IR group (p<0,05). Tissue MPO values in IR group showed a significant increase compared to Sham group, whereas in the IR+BG group there was not a significant decrease. Also, there was not a significant difference in tissue catalase levels. Tissue GSH values showed a significant decrease in IR group compared to Sham group (p<0,05). In the IR+BG group a significant increase was found compared to IR group (p<0,05). Less damage has been revealed in the IR+BG group compared to IR group in histopathologic examination.

Conclusion: All these data show that beta glucan may have an antioxidant effect on renal ischemia reperfusion injury.

Anahtar Kelimeler

Antioxidant, Beta glucan, İschemia, Kidney, Reperfusion

Destekleyen Kurum

Aydin Adnan Menderes University, Scientific Research Projects Committee

Proje Numarası

TPF-16034

Teşekkür

Authors, thanks to Histology and Embryology Department, Aydin Adnan Menderes University, Faculty of Medicine for their contribution.

Beta Glukanin Deneysel Böbrek İskemi Reperfüzyon Hasarina Karşi Koruyucu Etkileri

Öz

Amaç: Bu çalışma, böbrek dokusunda iskemi ve reperfüzyon hasarının neden olduğu oksidatif strese karşı beta glukanın olası koruyucu etkilerini araştırmayı amaçlamaktadır.

Yöntem: Çalışmada 300-350 gr ağırlığında 30 adet erkek Wistar albino sıçan kullanıldı (n=10). Sıçanlar rastgele Sham kontrol, iskemi reperfüzyon grubu (İR), iskemi reperfüzyon + beta glukan grubu (IR + BG) olmak üzere üç gruba ayrıldı. Sham grubuna sol nefrektomi yapıldı, sağ böbrek histopatolojik ve biyokimyasal inceleme için alındı. İR grubunda sol nefrektomi sonrası travmatik olmayan mikrovasküler klemp ile 45 dakika iskemi prosedürü uygulandı, ardından sağ böbrekte 60 dakika reperfüzyon uygulandı. IR+BG grubunda, sıçanlara 10 gün süreyle 100 mg/kg beta glukan gastrik gavaj yoluyla uygulandı. Anestezi altında sağ böbreğe 45 dakikalık iskemi sonrası 60 dakika reperfüzyon uygulandı.

Bulgular: Biyokimyasal inceleme sonucunda MDA değerleri IR grubunda Sham grubuna göre anlamlı artış gösterdi (p<0,05). IR+BG grubunda IR grubuna göre anlamlı azalma oldu (p<0,05). IR grubunda doku MPO değerlerinde Sham grubuna göre anlamlı bir artış görülürken, IR+BG grubunda anlamlı bir azalma olmadı. Ayrıca doku katalaz seviyelerinde de anlamlı bir fark yoktu. Doku GSH değerleri IR grubunda Sham grubuna göre anlamlı düşüş gösterdi (p<0,05). IR+BG grubunda IR grubuna göre anlamlı artış bulundu (p<0,05). Histopatolojik incelemede IR+BG grubunda IR grubuna göre daha az hasar saptandı.

Sonuç: Tüm bu veriler, beta glukanın renal iskemi reperfüzyon hasarı üzerinde antioksidan etkiye sahip olabileceğini göstermektedir.

Anahtar Kelimeler

Antioksidan, Beta glukan, İskemi, Böbrek, Reperfüzyon

Proje Numarası

TPF-16034

Kaynakça

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