Gastroprotective effect of tarantula cubensis extract in the indomethacin-induced peptic ulcer model in rats

Yıl 2020, Cilt: 4 Sayı: 3, 278 - 284, 29.10.2020

Fatma Nihan Cankara , Özlem Özmen , Hasan Basri Savaş , Yasemin Şahin , Caner Günaydın

https://doi.org/10.30565/medalanya.797406

Cited By: 4

Öz

Aim: Gastric ulcers are the most common gastrointestinal disease, due to several factors in the industrialized world. They may also occur after many pharmacological agents, combined with gastroprotective agents such as proton pump inhibitors (PPI) or anti-acids, are used as a preferred approach to maintaining gastrointestinal health. Unexpected adverse effects in these combinations make natural products an important alternative option.

Methods: Therefore, the main aim of this study is to investigate one natural compound, the Tarantula cubensis extract (TCE), in the experimental peptic ulcer model which was created by a single administration of indomethacin (40 mg/kg, body weight) to fed state Wistar-albino rats. The animals were pre-treated with two-doses of the TCE (0.2 ml/kg) before the indomethacin administration. After six hours, they were euthanized and the stomach tissue was isolated for biochemical and immunohistochemical analysis. Total antioxidant status/total oxidant status (TAS/TOS), prostaglandin E2 (PGE2), and nuclear factor kappa-B (NF-κB) levels were determined with ELISA in tissue homogenates. Caspase-3, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-α), were visualized with immunohistochemistry in intact tissues.

Results: Pre-treatment with TCE increased PGE2 levels and decreased total oxidative status (TAS/TOS). Additionally, TCE alleviated the increase of (NF-κB) levels due to the indomethacin administration. Histopathological and immunostaining results showed that TCE mitigated elevated immunoreactivity of the caspase-3, COX-2, iNOS, and TNF-α which were the results of the indomethacin administration.

Conclusion: Our study demonstrated that pre-TCE treatment ameliorated indomethacin-induced peptic ulcers via antioxidant and anti-inflammatory actions.

Anahtar Kelimeler

Tarantula Cubensis Extract, Indomethacin, Peptic Ulcer, Oxidative stress, Inflammation

Sıçanlarda indometazin ile indüklenmiş peptik ülser modelinde tarantula cubensis ekstraktının gastroprotektif etkisi

Öz

Amaç: Gastrik ülser, endüstrileşmiş dünyada çeşitli faktörler nedeniyle en sık görülen gastrointestinal hastalıktır. Bununla birlikte mide ülseri, gastrointestinal sağlığın korunması için genellikle tercih edilen proton pompa inhibitörleri (PPI) veya anti-asitler gibi birçok gastroprotektif farmakolojik ajanın kombine kullanılmasından sonra da nüks edebilmektedir. Bu kombinasyonlardaki beklenmedik olumsuz etkiler doğal ürünleri alternatif bir seçenek haline getirir. Bu nedenle, bu çalışmanın temel amacı, tek bir indometasin (40 mg/kg) ile oluşturulan deneysel peptik ülser modelinde doğal bileşiklerden biri olan Tarantula cubensis (TCE) ekstraktının etkisinin araştırılmasıdır.

Yöntemler: İndometazin uygulanmasından önce iki doz TCE (0.2 ml/kg) s.k yoldan uygulanmıştır. Tek doz Indometazin (40 mg/kg) uygulamasından altı saat sonra anestezi altında mide dokusu biyokimyasal ve immünohistokimyasal analiz için çıkarılmıştır. Total antioksidan durum/total oksidan durum (TAS/TOS), prostaglandin E2 (PGE2) ve nükleer faktör kappa-B (NF-κB) düzeyleri doku homojenatlarında ELISA ile belirlenmiştir. Kaspaz-3, siklooksijenaz-2 (COX-2), indüklenebilir nitrik oksit sentaz (iNOS) ve tümör nekroz faktörü-alfa (TNF-α) immünohistokimya ile görüntülenmiştir.

Bulgular: TCE ile ön tedavi PGE2 düzeylerini artırdı ve toplam oksidatif durumu (TAS / TOS) azaltmıştır. Ek olarak, TCE indometazin uygulamasına bağlı olarak NF-κB düzeylerindeki artışı hafifletmiştir. Histopatolojik ve immünboyama sonuçları, TCE'nin, indometazin uygulamasının sonuçları olan kaspaz-3, COX-2, iNOS ve TNF-α'nın yüksek immünoreaktivitesini azalttığını göstermiştir.

Sonuç: Çalışmamız TCE tedavisinin, antioksidan ve antiinflamatuvar etkiler yoluyla indometazine bağlı peptik ülserleri iyileştirdiğini göstermiştir.

Anahtar Kelimeler

Tarantula Cubensis Ekstraktı, İndometazin, Peptik Ülser, Oksidatif stres, İnflamasyon

Kaynakça

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