Klinik Araştırma
BibTex RIS Kaynak Göster

Investigation of the Sox-9 and Caspase-6 Immune Activity in Placentas of Pregnant Women with GDM

Yıl 2023, Cilt: 5 Sayı: 3, 445 - 8, 18.09.2023
https://doi.org/10.37990/medr.1252256

Öz

Aim: In this study, we investigated the immunohistochemical staining of Sox-9 and Caspase-6 expression in placentas of pregnant woman with gestational diabetes mellitus (GDM).
Material and Methods: Placentas of 20 healthy and 20 women with GDM were processed for routine histological tissue processing. The biochemical and clinical parameters of patients were recorded. Placentas were stained with hematoxylin-eosin and Sox-9 and Caspase-6 immunostaining.
Results: In control group, Sox-9 expression was negative in decidual and connective cells and endothelial cells. In GDM group, Sox-9 expression was increased especially in the decidual cells. For the Caspase-6 expression, Caspase-6 reaction was mainly in maternal region in control group. In GDM group, Caspase-6 reaction was increased in decidual cells, in endothelial cells and in the syncytial nodes.
Conclusion: Expression of Sox-9 transformed the decidua cells and lead to apoptotic pathway via Caspase-6 expression.

Kaynakça

  • Mitra M, Clements KM, Zhang J, et al. Maternal characteristics, pregnancy complications, and adverse birth outcomes among women with disabilities. Med Care. 2015;53:1027-32.
  • Ko JY, DeSisto CL, Simeone RM, et al. Adverse pregnancy outcomes, maternal complications, and severe illness among us delivery hospitalizations with and without a coronavirus disease 2019 (COVID-19) diagnosis. Clin Infect Dis. 2021;73:24-31.
  • Qin J, Wang H, Sheng X, et al. Pregnancy-related complications and adverse pregnancy outcomes in multiple pregnancies resulting from assisted reproductive technology: a meta-analysis of cohort studies. Fertil Steril. 2015;103:1492-508.
  • Neiger R. Long-term effects of pregnancy complications on maternal health: A review. J Clin Med. 2017;6:76. McIntyre HD, Catalano P, Zhang C, et al. Gestational diabetes mellitus. Nat Rev Dis Primers. 2019;11:47.
  • Minschart C, Beunen K, Benhalima K. An update on screening strategies for gestational diabetes mellitus: A narrative review. Diabetes Metab Syndr Obes. 2021;14:3047-76.
  • Plows JF, Stanley JL, Baker PN, et al. The pathophysiology of gestational diabetes mellitus. Int J Mol Sci. 2018;19:3342.
  • Kampmann U, Madsen LR, Skajaa GO, et al. Gestational diabetes: A clinical update. World J Diabetes. 2015;6:1065-72.
  • Aşır F, Nergiz Y, Pala A. Vitamin E protected the mouse adrenal gland against immobilization stress. Pol J Vet Sci. 2022;25:447-54.
  • Durgun C, Aşır F. Effect of ellagic acid on damage caused by hepatic ischemia reperfusion in rats. Eur Rev Med Pharmacol Sci. 2022;26:8209-15.
  • Aldahmash WM, Alwasel SH, Aljerian K. Gestational diabetes mellitus induces placental vasculopathies. Environ Sci Pollut Res Int. 2022;29:19860-8.
  • Rudge MV, Lima CP, Damasceno DC, et al. Histopathological placental lesions in mild gestational hyperglycemic and diabetic women. Diabetol Metab Syndr. 2011;3:19.
  • Jo A, Denduluri S, Zhang B, et al. The versatile functions of Sox9 in development, stem cells, and human diseases. Genes Dis. 2014;1:149-61.
  • Aguilar-Medina M, Avendaño-Félix M, Lizárraga-Verdugo E, et al. Sox-9 Stem-cell factor: clinical and functional relevance in cancer. J Oncol. 2019;2019:6754040.
  • Sekido R, Bar I, Narváez V, et al. Sox-9 is up-regulated by the transient expression of SRY specifically in Sertoli cell precursors. Dev Biol. 2004;274:271-9.
  • Zhao J, Patel J, Kaur S, et al. Sox9 and Rbpj differentially regulate endothelial to mesenchymal transition and wound scarring in murine endovascular progenitors. Nat Commun. 2021;12:2564.
  • Xian L, Georgess D, Huso T, et al. HMGA1 amplifies Wnt signaling and expands the intestinal stem cell compartment and Paneth cell niche. Nat Commun. 2017;8:15008.
  • Zheng M, Karki R, Vogel P, Kanneganti TD. Caspase-6 is a key regulator of innate immunity, inflammasome activation, and host defense. Cell. 2020;181:674-87.
  • Beskisiz S, Aşır F. Hesperidin may protect gastric tissue against immobilization stress. Anal Quant Cytopathol Histopathol. 2021;43:399-405.
  • Cheng SB, Nakashima A, Huber WJ, et al. Pyroptosis is a critical inflammatory pathway in the placenta from early onset preeclampsia and in human trophoblasts exposed to hypoxia and endoplasmic reticulum stressors. Cell Death Dis. 2019;10:927.
  • Mu J, Kanzaki T, Si X, et al. Apoptosis and related proteins in placenta of intrauterine fetal death in prostaglandin f receptor-deficient mice, Biol Reprod. 2003;68:1968–74.
Yıl 2023, Cilt: 5 Sayı: 3, 445 - 8, 18.09.2023
https://doi.org/10.37990/medr.1252256

Öz

Kaynakça

  • Mitra M, Clements KM, Zhang J, et al. Maternal characteristics, pregnancy complications, and adverse birth outcomes among women with disabilities. Med Care. 2015;53:1027-32.
  • Ko JY, DeSisto CL, Simeone RM, et al. Adverse pregnancy outcomes, maternal complications, and severe illness among us delivery hospitalizations with and without a coronavirus disease 2019 (COVID-19) diagnosis. Clin Infect Dis. 2021;73:24-31.
  • Qin J, Wang H, Sheng X, et al. Pregnancy-related complications and adverse pregnancy outcomes in multiple pregnancies resulting from assisted reproductive technology: a meta-analysis of cohort studies. Fertil Steril. 2015;103:1492-508.
  • Neiger R. Long-term effects of pregnancy complications on maternal health: A review. J Clin Med. 2017;6:76. McIntyre HD, Catalano P, Zhang C, et al. Gestational diabetes mellitus. Nat Rev Dis Primers. 2019;11:47.
  • Minschart C, Beunen K, Benhalima K. An update on screening strategies for gestational diabetes mellitus: A narrative review. Diabetes Metab Syndr Obes. 2021;14:3047-76.
  • Plows JF, Stanley JL, Baker PN, et al. The pathophysiology of gestational diabetes mellitus. Int J Mol Sci. 2018;19:3342.
  • Kampmann U, Madsen LR, Skajaa GO, et al. Gestational diabetes: A clinical update. World J Diabetes. 2015;6:1065-72.
  • Aşır F, Nergiz Y, Pala A. Vitamin E protected the mouse adrenal gland against immobilization stress. Pol J Vet Sci. 2022;25:447-54.
  • Durgun C, Aşır F. Effect of ellagic acid on damage caused by hepatic ischemia reperfusion in rats. Eur Rev Med Pharmacol Sci. 2022;26:8209-15.
  • Aldahmash WM, Alwasel SH, Aljerian K. Gestational diabetes mellitus induces placental vasculopathies. Environ Sci Pollut Res Int. 2022;29:19860-8.
  • Rudge MV, Lima CP, Damasceno DC, et al. Histopathological placental lesions in mild gestational hyperglycemic and diabetic women. Diabetol Metab Syndr. 2011;3:19.
  • Jo A, Denduluri S, Zhang B, et al. The versatile functions of Sox9 in development, stem cells, and human diseases. Genes Dis. 2014;1:149-61.
  • Aguilar-Medina M, Avendaño-Félix M, Lizárraga-Verdugo E, et al. Sox-9 Stem-cell factor: clinical and functional relevance in cancer. J Oncol. 2019;2019:6754040.
  • Sekido R, Bar I, Narváez V, et al. Sox-9 is up-regulated by the transient expression of SRY specifically in Sertoli cell precursors. Dev Biol. 2004;274:271-9.
  • Zhao J, Patel J, Kaur S, et al. Sox9 and Rbpj differentially regulate endothelial to mesenchymal transition and wound scarring in murine endovascular progenitors. Nat Commun. 2021;12:2564.
  • Xian L, Georgess D, Huso T, et al. HMGA1 amplifies Wnt signaling and expands the intestinal stem cell compartment and Paneth cell niche. Nat Commun. 2017;8:15008.
  • Zheng M, Karki R, Vogel P, Kanneganti TD. Caspase-6 is a key regulator of innate immunity, inflammasome activation, and host defense. Cell. 2020;181:674-87.
  • Beskisiz S, Aşır F. Hesperidin may protect gastric tissue against immobilization stress. Anal Quant Cytopathol Histopathol. 2021;43:399-405.
  • Cheng SB, Nakashima A, Huber WJ, et al. Pyroptosis is a critical inflammatory pathway in the placenta from early onset preeclampsia and in human trophoblasts exposed to hypoxia and endoplasmic reticulum stressors. Cell Death Dis. 2019;10:927.
  • Mu J, Kanzaki T, Si X, et al. Apoptosis and related proteins in placenta of intrauterine fetal death in prostaglandin f receptor-deficient mice, Biol Reprod. 2003;68:1968–74.
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular İç Hastalıkları
Bölüm Özgün Makaleler
Yazarlar

Ece Öcal 0000-0002-7577-7012

Senem Alkan Akalın 0000-0001-8286-2824

Engin Deveci 0000-0002-2353-1184

Erken Görünüm Tarihi 25 Mayıs 2023
Yayımlanma Tarihi 18 Eylül 2023
Kabul Tarihi 3 Nisan 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 5 Sayı: 3

Kaynak Göster

AMA Öcal E, Alkan Akalın S, Deveci E. Investigation of the Sox-9 and Caspase-6 Immune Activity in Placentas of Pregnant Women with GDM. Med Records. Eylül 2023;5(3):445-8. doi:10.37990/medr.1252256

 Chief Editors

Assoc. Prof. Zülal Öner
Address: İzmir Bakırçay University, Department of Anatomy, İzmir, Turkey

Assoc. Prof. Deniz Şenol
Address: Düzce University, Department of Anatomy, Düzce, Turkey

E-mail: medrecsjournal@gmail.com

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