Chitin and Chitosan from Sepia officinalis: Extraction and Characterization Study

Öz

A cuttlebone sourced from Sepia officinalis, acquired from the Iskenderun fish market, served as the raw material for the extraction of chitin (CT) and chitosan (CS). The chemical structure and physico-chemical properties of both CT and CS were comprehensively characterized through Fourier-transform infrared (FTIR), X-ray diffractometry (XRD), and scanning electron microscopy (SEM). The yields of CT and CS obtained from the cuttlebone of S. officinalis were calculated as 32.1% and 72.6%, respectively. The results of FTIR spectrum analysis revealed the presence of functional groups at various bands, confirming the samples to be CT and CS. The deacetylation degree (DD) value was determined to be 84.20% based on the FTIR results. The crystal index (CrI) of CT obtained from the cuttlebone was calculated as 60.13%. Morphological distinctions between CT and CS biopolymers were emphasized by the results of SEM analysis. The successful extraction of these biopolymers from the cuttlebone of S. officinalis, species of significant commercial importance and widespread consumption, was accomplished. These biopolymers derived from the shells of marine animals can be utilized as sustainable and environmentally friendly materials in various applications. These have the potential to offer alternative and innovative solutions in various fields such as batteries, microwave electronics, packaging materials and the pharmaceutical industry.

Anahtar Kelimeler

• Alternative
• Biogenic residual
• Green biotechnology
• Biopolymer

Sepia officinalis’ten Kitin ve Kitosan: Ekstraksiyon ve Karakterizasyon Çalışması

Öz

İskenderun balık pazarından temin edilen Sepia officinalis kaynaklı mürekkep balığı kemiği, kitin (CT) ve kitosan (CS) üretiminde kullanıldı. Hem CT hem de CS'nin kimyasal yapısı ve fiziko-kimyasal özellikleri, Fourier-dönüşümlü kızılötesi (FTIR), X-ışını difraktometre (XRD) ve Taramalı Elektron Mikroskobu (SEM) aracılığıyla kapsamlı bir şekilde karakterize edildi. Mürekkep balığı kemiğinden elde edilen CT ve CS verimleri sırasıyla %32,1 ve %72,6 olarak hesaplandı. FTIR spektrum analizi sonuçları, çeşitli bantlarda işlevsel grupların varlığını ortaya koyarak örneklerin CT ve CS olduğunu doğruladı. Deasetilasyon derecesi (DD) değeri, FTIR sonuçlarına göre %84,20 olarak belirlendi. Mürekkep balığı kemiğinden elde edilen CT'nin kristal indeks (CrI) değeri %60,13 olarak hesaplandı. SEM analizi sonuçları, CT ve CS biyopolimerleri arasındaki morfolojik farklılıkları vurguladı. Bu önemli ticari öneme sahip ve yaygın tüketilen bir tür olan S. officinalis'in kemiğinden başarıyla elde edilen bu biyopolimerler, denizel canlıların kabuklarından elde edilen sürdürülebilir ve çevre dostu malzemeler olarak kullanılabilir. Bu malzemeler, piller, mikrodalga elektronikler, ambalaj malzemeleri ve ilaç endüstrisi gibi çeşitli alanlarda alternatif ve yenilikçi çözümler sunma potansiyeline sahiptir.

Anahtar Kelimeler

• Alternatif
• Biyojenik atık
• Yeşil Biyoteknoloji
• Biyopolimer

Kaynakça

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