Yetiştiriciliği Yapılan Mersin Balıklarından İzole Edilen Bakterilerin Antimikrobiyal Direnç Profillerinin Belirlenmesi

Öz

Türkiye'de mersin balıkları yetiştiriciliği son yıllarda artmaya başlamıştır. Balıklar yetiştiricilik aşamasında çeşitli hastalıklara maruz kalır ve bu hastalıklar ekonomik kayıplara sebep olabilmektedir. Bu çalışmada karaca (Acipenser gueldenstaedtii) ve sibirya (Acipenser baerii) mersin balıklarından ortalama ağırlıkları 3 kg’a ulaşıncaya kadar geçen sürede izole edilen toplam 37 suşun antimikrobiyal maddelere karşı direnç profilleri ve direnç genlerin varlığı araştırılmıştır. Bakterilerin en fazla dirençli oldukları antibiyotikler sulfametoksazol ve ampisilin olarak tespit edilmiştir (%97.3). Bunun yanında bakterilerin test edilen antibiyotikler arasında gentamisin, oksolinik asit, trimetoprim+sulfametoksazol ve florfenikole karşı hassasiyetleri sırasıyla %86.5, %59.5, %45.9 ve %40.5 olarak tespit edilmiştir. Bakterilerde taranan genlerden tetA ve tetB genleri sırasıyla % 10.8 ve 21.6 olarak tespit edilmiştir. En fazla tespit edilen genler % 24 oranla ampC ve cmlA genleri olmuştur. Bu genleri % 19’luk oranla sul3 ve floR genleri takip etmiştir. Gerçekleştirilen bu çalışmada, bakterilerin antibiyotiklere karşı direnç seviyelerinin oksitetrasiklin, streptomisin, enrofloksasin ve ampisilinde %50’den yüksek olması, mersin balığı yetiştiriciliğinde bakteriyel hastalıkların tedavisinde gelecekte sorunlar yaşanacağını söylenebilir.

Anahtar Kelimeler

• Antimikrobiyal,Antibiyogram,Direnç genleri,Mersin Balığı

Determination of Antimicrobial Resistance Profiles of the Bacteria Isolated from Cultured Sturgeons

Öz

Sturgeon farming in Turkey has increased in recent years. Fish are exposed to various diseases during the culturing period and these diseases can cause economic losses. In this study, a total of 37 strains isolated from diamond (Acipenser gueldenstaedtii) and siberian (Acipenser baerii) sturgeons till their average weights reach up to 3 kg were used to determine resistance profiles against antimicrobial substances and investigated their resistance genes. The most resistance to antibiotics in bacteria were found as sulphamethoxazole and ampicillin (97.3%). Moreover, the susceptibility of bacteria to the tested antibiotics for gentamicin, oxolinic acid, trimethoprim + sulfamethoxazole and florfenicol was determined as 86.5%, 59.5%, 45.9% and 40.5%, respectively. tetA ve tetB genes in the tested bacteria were detected as 10.8% and 21.6%, respectively. The most common genes were ampC and cmlA genes (24%) followed by sul3 and floR genes with a ratio of 19%. In this study, it can be said that there may be problems in the treatment of bacterial diseases in sturgeon farming because of being higher than 50% of the resistance levels of bacteria against antibiotics such as oxytetracycline, streptomycin, enrofloxacin and ampicillin.

Anahtar Kelimeler

• Antimicrobial,Antibiogram,Resistance Genes

Kaynakça

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