Farklı Su Sıcaklıklarında Gül Ağacı (Aniba rosaeodora) Esansiyel Yağının ve 2-fenoksietanolün Japon Balığı (Carassius auratus) Üzerindeki Anestezik Etkileri

Yıl 2023, Cilt: 9 Sayı: 2, 15 - 26, 28.12.2023

Barış Şahin , Volkan Kızak

https://doi.org/10.58626/menba.1372338

Öz

Bu çalışmada, gül ağacı esansiyel yağının ve 2-fenoksietanolün farklı su sıcaklıklarında Japon balıkları üzerinde anestezik etkilerinin incelenmesi amaçlanmıştır. 14°C ve 20°C su sıcaklıklarında anestezik denemeler gerçekleştirilmiştir. Gül ağacı esansiyel yağı (ArEO) için 250, 300 ve 350 µl/lt, 2-fenoksietanol (2-PE) için ise 600, 700 ve 800 µl/lt konsantrasyonlar denenmiştir. 14°C’de ideal ArEO konsantrasyonu 300 µl/lt, 20°C’de ise 250 µl/lt olarak belirlenmiştir. 2-PE için her iki su sıcaklığında da ideal konsantrasyon 800 µl/lt olarak tespit edilmiştir. ArEO ve 2-PE’ün yüksek konsantrasyonları, daha kısa indüksiyon sürelerine ve daha uzun iyileşme sürelerine neden olmuştur. Anestezik ajanların konsantrasyonları indüksiyon süreleri ile negatif, iyileşme süreleri ile pozitif korelasyon göstermiştir. ArEO için 14ºC’de AD ve RF süreleri arasında zayıf negatif bir ilişki, 20ºC’de ise negatif bir ilişki bulunmuştur. 14ºC ve 20ºC’lerde 2-PE anesteziğinde AD ve RF süreleri arasında negatif ilişki tespit edilmiştir. ArEO AD süreleri ve ArEO konsantrasyonları arasında 14ºC ve 20ºC’lerde kuvvetli negatif bir ilişki vardır ve benzer bir ilişki 2-PE için de bulunmuştur. Bununla birlikte, farklı su sıcaklıklarında her iki anestezik ajanda RF süreleri ve konsantrasyonlar arasında kuvvetli pozitif ilişki tespit edilmiştir. Artan su sıcaklığının bir sonucu olarak her iki anestezik maddede anestezi ve iyileşme sürelerinin kısaldığı görülmüştür. ArEO ve 2-PE için indüksiyon ve iyileşme sürelerinde su sıcaklığı ve anestezik konsantrasyon interaksiyonu önemli bulunmuştur.

Anahtar Kelimeler

Anestezi, su sıcaklığı, Japon balığı, Aniba rosaeodora

Etik Beyan

T.C. Munzur Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu’nun E-63614754-050.01.04-2300006839 sayılı kararı (Toplantı sayısı 2022-21 / Karar no. 21-01) ile etik kurallar çerçevesinde bu çalışma gerçekleştirilmiştir.

Teşekkür

Bu araştırma makalesi, Barış Şahin’in yüksek lisans tezinden üretilmiştir.

Kaynakça

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