Farklı Sıcaklıklara Maruz Kalan Balık Solungaç Dokularındaki Değişimlerin Histolojik Olarak İncelenmesi

Yıl 2024, Cilt: 10 Sayı: 3, 47 - 57, 28.12.2024

Şeyma Nur Azboy , Hafize Barik , Kader Alkan Zehra Alkan Çekiç , Ahmet Regaib Oğuz

https://doi.org/10.58626/menba.1513525

Öz

İklim değişikliği balık metabolizmasını etkileyen çevresel faktörler üzerinde de etkiye sahiptir. Suyun sıcaklığı arttıkça balığın aldığı besin, oksijen alınımı ve üreme gibi süreçlerde değişimlere neden olmaktadır. Balıklar içinde bulundukları suların fiziksel ve kimyasal özelliklerinden etkilenmektedir. Bu değişimler davranışsal, histolojik ve moleküller düzeyde olabilmektedir. Solungaçlar su ile direkt temas halinde oldukları için kirleticiler, fizikokimyasal değişimler, parazitik ve mikrobiyal enfeksiyonlar gibi çevresel değişimlerden etkilenen ilk organlardan biridir. Bu nedenle Pseudotropheus acei balıkları farklı sıcaklıklara maruz bırakılarak histolojik olarak incelendi. Sıcaklık artışına bağlı olarak solungaç dokusunda lamellar ayrılma, nekroz ve hiperplazi gibi histopatolojik değişimler gözlendi. Sıcak stresine maruz kalan Pseudotropheus acei balığı solungaç dokusunda bulunan mukus hücre içerik ve yoğunluklarında farklılıklar olduğu gözlendi. Nötral, karboksilatlı, O-sülfat esterli, güçlü sülfatlı ve sülfatlanmış glikokonjugatların yoğunluklarının sıcaklık artışına bağlı olarak artış gösterdiği belirlendi. Özellikle sıcaklık artışının ilk maruziyeti olan 29 ºC ve 30 ºC de mukus yoğunluğu diğer gruplara göre fazlaydı. Yapılan boyamalar sonucunda semikantitatif olarak sıcaklığın mukus hücre değişimi üzerine etkisinin olduğu gözlendi.

Anahtar Kelimeler

Mukus, Histoloji, Sıcaklık, Solungaç

Etik Beyan

YUHADYEK 2023/05-35

Destekleyen Kurum

TÜBİTAK 2209-A üniversite öğrencileri araştırma destek programı

Proje Numarası

1919B012215262

Teşekkür

TÜBİTAK 2209-A üniversite öğrencileri araştırma destekleme programına teşekkürlerimizi sunarız.

Kaynakça

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