Pelargonium quercetorum Agnew. bitkisinin antioksidan aktivitesinin belirlenmesi

Yıl 2018, Cilt: 11 Sayı: 2, 174 - 183, 15.08.2018

Gökçe Şeker Karatoprak , Mehmet Fırat Müberra Koşar

https://doi.org/10.26559/mersinsbd.398926

Cited By: 2

Öz

Amaç: Antiparaziter olarak geleneksel kullanımı
mevcut olan Geraniaceae familyasına dahil Pelargonium
quercetorum Agnew.’in toplam fenolik madde miktarı ve antioksidan
aktivitesinin araştırılması amaçlanmıştır.
Yöntem: P. quercetorum'un toprak
altı kısımlarından hazırlanan %70 metanol ve %11 etanol ekstreleri ile toprak
üstü kısmından hazırlanan %70 metanol ekstresinin toplam fenolik, flavonoit ve
flavonol içerikleri spektrofotometrik olarak incelenmiştir. Ekstrelerin
antioksidan aktivitelerini belirlemek amacıyla 1.1-difenil-2-pikrilhidrazil
(DPPH^l) ve 2.2’-azino-bis (3-etilbenzotiazolin-6-
sulfonik asit) (ABTS^+l)
radikal süpürücü etkileri, Fe(III)’ü Fe(II)’ye
indirgeme özellikleri ve demir(II) şelat (kelat) aktiviteleri
incelenmiştir. Bulgular ve Sonuç: %70
Metanol kök ekstresinin toplam fenolik ve flavonoit (242.29 ± 5.52 mg_GAE/g_ekstre,
64.95 ± 2.93mg_CA/g_ekstre) içerik bakımından
zengin olduğu ve antioksidan aktivite deneylerinde de güçlü etki gösterdiği
belirlenmiştir.

Anahtar Kelimeler

Geraniaceae, Pelargonium quercetorum, antioksidan aktivite

Kaynakça

• 1. Matkowski A, Zielinska S, Oszmianski J, Lamer-Zarawska E. Antioxidant activity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim., and S. verticillata L. Bioresour Technol 2008; 99: 7892–7896.
• 2. Larson RA. Naturally occuring antioxidants. Lewis Publishers, New york 1997; pp 1-15.
• 3. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994; 344:721–724.
• 4. Pincemail JJ. Free radicals and antioxidants in human diseases. In: Favier AE, Cadet J, Kalyanaraman B, Fontecave M, Pierre JL. (eds.), Analysis of Free Radicals in Biological Systems. Birkhauser Verlag, Berlin 1995, pp 83–98.
• 5. Dreher D, Junod AF. Role of oxygen free radicals in cancer development. Eur. J. Cancer 1996; 32A: 30–38.
• 6. Oberley TD, Oberley LW. Antioxidant enzyme levels in cancer. Histol. Histopathol 1997; 12: 525–535.
• 7. Maxwell S. Anti-oxidant therapy: does it have a role in the treatment of human disease? Exp Opin Invest Drugs 1997; 6: 211–236.
• 8. Brendlera T, Van Wyk BE. A historical, scientific and commercial perspective on the medicinal use of Pelargonium sidoides (Geraniaceae). J Ethnopharmacol 2008; 119: 420–433.
• 9. Trun W, Kiderlen AF, Kolodziej H. Nitric oxide synthase and cytokines gene expression analyses in Leishmania-infected RAW 264.7 cells treated with an extract of Pelargonium sidoides (Eps 7630). Phytomed 2006; 13: 570–575. 10. Davis PH, Mill RR, Kit T. Pelargonium L’Hérit In: Flora of Turkey and the East Aegean Islands, Davis PH (eds), University Press, Edinburgh, 1988; 10: pp 106
• 11. Uce İ, Tunçtürk M. Hakkâri’de Doğal Olarak Yetişen ve Yaygın Olarak Kullanılan Bazı Yabani Bitkiler. Biyoloji Bilimleri Araştırma Dergisi 2014; 7 (2): 21-25.
• 12. Taherpour AA, Maroofı H, Kheradmand K. Chemical composition of the essential oil of Pelargonium quercetorum Agnew. of Iran. Nat Prod Res 2007; 21(1): 24–27.
• 13. Kolodziej H, Kiderlen AF. In vitro evaluation of antibacterial and immunomodulatory activities of Pelargonium reniforme, Pelargonium sidoides and the related herbal drug preparation EPs® 7630. Phytomedicine 2007; 14 (6): 18-26.
• 14. Singleton VL, Orthofer R, Lamuela-Ravento´S RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. In Methods in Enzymology, Packer L (ed.), Academic Press, San Diego, CA, 1999; 299: 152-315.
• 15. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64: 555−559.
• 16. Miliauskas G, Venskutonis PR, Van-Beek TA. Screening of Radical Scavenging Activity of Some Medicinal and Aromatic Plant Extracts. Food Chem 2004; 85: 231-237.
• 17. Oyaizu M. Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986; 44: 307-315.
• 18. Gyamfi MA, Yonamine M, Aniya Y. Free-Radical Scavenging Action of Medicinal Herbs from Ghana Thonningia Sanguinea on Experimentally Induced Liver Injuries. Gen Pharmacol 1999; 32: 661-667.
• 19. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Bio Med 1999; 26: 1231-1237.
• 20. Carter P. Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Anal Biochem 1971; 40: 450–458.
• 21. Çakal S. Pelargonium endlicherianum Fenzl.(Geraniaceae) kök ekstrelerinin UMCA® preparatı ile karşılaştırılmalı araştırılması, Yüksek Lisans Tezi, Anadolu Üniversitesi, Sağlık Bilimleri Enstitüsü, Eskişehir 2009, ss 15-27.
• 22. Tepe B, Sökmen M, Akpulat HA, Yumrutaş Ö, Sökmen A. Screening of antioxidative properties of the methanolic extracts of Pelargonium endlicherianum Fenzl. Verbascum wiedemannianum Fisch. & Mey. Sideritis libanotica Labill. subsp. linearis (Bentham) Borm. Centaurea mucronifera DC. and Hieracium cappadocicum Freyn. from Turkish flora. Food Chem 2006; 98: 9–13.
• 23. Lalli YY, Van Zyl RL, Van Vuuren SF, Viljoen AM. In vitro biological activities of South African Pelargonium (Geraniaceae) species. S Afr J Bot 2008; 74: 153–157.
• 24. Arı F, Çelikler S, Karakaş D, Cevatemre B, Ulukaya E. Total Phenolic Content, Antioxidant and Cyto/Genotoxic Activities of Pelargonium quercetorum Agnew in Human Breast Cancer Cells. Journal of Clinical and Experimental Investigations 2017; 8: 22-30.
• 25. Adewusi EA, Afolayan AJ. Antibacterial, antifungal and antioxidant activity of the roots and leaves of Pelargonium reniforme Curtis (Geraniaceae). Afr J Biotechnol 2009; 8 (22): 6425-6433.
• 26. Walia H, Kumar S, Arora S. Effect of fractionation on in vitro antiradical efficacy of acetone extract of Terminalia chebula. Afr J Pharm Pharmacol 2014; 8(11): 311-320.
• 27. Shahidi F. Natural Antioxidants: Chemistry, Health Effects, and Applications, AOCS Press, United States of America 1997; pp 98.