Pelargonium endlicherianum fenzl. uçucu yağı ve penisilin kombinasyonlarının streptoccus pneumoniae'ye karşı sinerjik potansiyeli

Yıl 2020, Cilt: 13 Sayı: 3, 371 - 381, 05.12.2020

Berrak Dumlupınar Gökçe Şeker Karatoprak

https://doi.org/10.26559/mersinsbd.718890

Öz

Amaç: Pelargonium endlicherianum uçucu yağının enfeksiyon tedavilerinde sıklıkla kullanılan benzilpenisilin ile kombine edilerek in vitro deney modeli içerisinde Streptoccus pneumoniae’ye karşı antimikrobiyal etkisinin ortaya çıkarılması ve insan lökosit hücreleri üzerindeki fonksiyonun tespit edilmesi amaçlandı. Yöntem: P. endlicherianum uçucu yağının benzilpenisilin ile kombine kullanımının Streptococcus pneumoniae’ye karşı antimikrobiyal etkisi agar difüzyon tekniği kullanılarak ve lökosit hücreleri üzerindeki fagositik fonksiyonları in vitro hücre deney modeli içinde insan lökosit hücresi WBC 264-9C ATCC HB-8902 kullanılarak ortaya çıkarılmıştır. P. endlicherianum uçucu yağ ve benzilpenisilinin bakterisidal etkisi zamana bağlı öldürme yöntemi ile zamana ve antibiyotik yoğunluğuna bağlı olarak dinamik olarak ortaya konuldu. Uçucu yağın tek başına ve antibiyotik ile kombine kullanıldığında bakteriyel dış membrandaki geçirgenliği U.V. spektrofotometre ve morfolojik görüntüsü taramalı elektron mikroskobu ile analiz edildi. Bulgular: Tedavi sonrası 24. saatte, ilaç kombinasyonu sadece uçucu yağ tedavisi ile karşılaştırıldığında, yaşayabilir hücre sayısında azalma olduğu gözlendi. Uçucu yağ+antibiyotik kombinasyonlarında uçucu yağ ve antibiyotik arasında sinerjizm gözlenmiş olup, lökosit hücrelerinin fagositik aktivitesi artarak canlı bakteri sayısında belirgin bir düşüş belirlendi. Sonuç: Çalışmanın sonuçlarına göre S. pneumoniae’ye karşı kullanılan benzilpenisilinin P. endlicherianum uçucu yağı ile kombine kullanımı sonucu antibiyotik etkinliğinin arttığı ve böylece bakterilerin antibiyotiklere karşı direnç geliştirmesi engellenerek etkin bir tedavi sağlanabileceği gösterilmiştir.

Anahtar Kelimeler

Pelargonium endlicherianum Fenzl., uçucu yağ, benzilpenisilin, antimikrobiyal aktivite

Destekleyen Kurum

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TUBİTAK)

Proje Numarası

216S666

Teşekkür

Bu çalışmada, 216S666 projesine mali destek sağladığı için Türkiye Bilimsel ve Teknolojik Araştırma Kurumu'na (TÜBİTAK) teşekkür ederiz. Çalışmamıza istatiksel olarak destek sağlayan Eda Merve Kurtuluş’a teşekkür ederiz.

Synergic potential of pelargonium endlicherianum fenzl. essential oil and penicillin combination against streptoccus pneumoniae

Öz

Aim: The main aim of the study is to combine Pelargonium endlicherianum essential oil with benzylpenicillin used in the treatment of infection, revealing the antimicrobial effect against S. pneumoniae in human in vitro experimental model and its functions on human leukocyte cells. Method: The antimicrobial effect of the use of P. endlicherianum essential oil combined with benzylpenicillin against Streptococcus pneumoniae was demonstrated using agar diffusion technique and its phagocytic functions on leukocyte cells in an in vitro cell assay model using the human leukocyte cell WBC 264-9C ATCC HB-8902. Bactericidal effect of essential oil and antibiotic combination was dynamically detected by the method of Time Kill assay, was analyzed by U.V spectrophotometer and morphologic images was analysed by scanning electron microscopy. Results: A reduction in the number of viable cells was observed at 24 hours after treatment, when the drug combination was compared only with essential oil treatment. In essential oil + antibiotic combination, synergism was observed between essential oil and antibiotic, and the phagocytic activity of leukocyte cells increased, and a significant decrease in the number of live bacteria was determined. Conclusion: According to the results, the use of benzylpenicillin, which are used in infection treatments, together with a phytochemical P. endlicherianum essential oil, increases the effectiveness of antibiotic and thus, it has been shown that an effective treatment can be achieved by preventing bacteria from developing resistance to antibiotics.

Anahtar Kelimeler

Pelargonium endlicherianum Fenzl, essential oil, benzylpenicillin, antimicrobial activity

Proje Numarası

216S666

Kaynakça

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