Effect of Vermicompost and Liquid Seaweed Extract Applications on Microbial Respiration and β-glucosidase Activity in Barley (Hordeum vulgaris L.) Rhizosphere

Öz

Since the use of chemical fertilizers disrupts the structure of the soil and negatively affects sustainable plant growth, the use of organic fertilizers has gained importance as an alternative to the use of chemical fertilizers. In our study, the effects of liquid seaweed fertilizer and vermicompost fertilizer on barley growth and root zone microbiological properties were investigated. The experiment was carried out in the greenhouse according to the randomized plot design with 3 replications. 4 doses of vermicompost (control, 10%, 20% and 40%), 4 doses of liquid seaweed fertilizer (control, 1%, 2% and 3%) and the combination of vermicompost and liquid seaweed fertilizer were applied to the soil. The plants were harvested 60 days after sowing. The applications increased the green parts and root weight, plant height and root length. The chlorophyll contents of the leaves increased with the applications of vermicompost and liquid seaweed fertilizer compared to the control. Increasing doses of vermicompost and liquid seaweed fertilizer significantly increased the microbial respiration and β-glucosidase activity in the rhizosphere compared to the control, the highest increase was obtained in the interaction of 40% vermicompost and 3% seaweed fertilizer.

Anahtar Kelimeler

• Vermicompost
• Liquid seaweed fertilizer
• Barley
• β-glucosidase
• Microbial soil respiration

Destekleyen Kurum

Harran Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

Bu araştırmanın yapılmasında maddi destek sağlayan Harran Üniversitesi Bilimsel Araştırma Projeleri Birimine (HÜBAP-21256) teşekkürlerimizi sunarız.

Etik Beyan

“Vermikompost ve Sıvı Deniz Yosunu Ekstraktı Uygulamalarının Arpa (Hordeum vulgaris L.) Rizosferinde Mikrobiyal Solunum ve β-glukosidaz Aktiviteye Etkisi” başlıklı çalışmanın yazım sürecinde bilimsel kurallara, etik ve alıntı kurallarına uyulmuş; toplanan veriler üzerinde herhangi bir tahrifat yapılmamış ve bu çalışma herhangi başka bir akademik yayın ortamına değerlendirme için gönderilmemiştir. Çalışmamızda herhangi bir canlıya ait veri bulunmadığından etik kurul izni gerekmemektedir.

Teşekkür

Bu araştırmanın yapılmasında maddi destek sağlayan Harran Üniversitesi Bilimsel Araştırma Projeleri Birimine (HÜBAP-21256) teşekkürlerimizi sunarız.

Vermikompost ve Sıvı Deniz Yosunu Ekstraktı Uygulamalarının Arpa (Hordeum vulgaris L.) Rizosferinde Mikrobiyal Solunum ve β-glukosidaz Aktiviteye Etkisi

Öz

Kimyasal gübre kullanımı toprağın yapısını bozduğu ve sürdürülebilir bitki gelişimini olumsuz etkilediği için günümüzde kimyasal gübre kullanımına alternatif olarak organik gübre kullanımı önem kazanmıştır. Çalışmamızda sıvı yosun gübresi ve vermikompost gübresi kullanılarak arpa gelişimi ve kök bölgesi mikrobiyolojik özelliklerine etkisi incelenmiştir. Deneme serada tesadüf parselleri deneme desenine göre 3 tekrarlı olarak yürütülmüştür. Vermikompostun 4 dozu (kontrol, % 10, % 20 ve % 40), sıvı yosun gübresinin 4 dozu (kontrol, % 1, % 2 ve % 3) ve vermikompost ile sıvı yosun gübresinin kombinasyonu ayrı ayrı topraklara uygulanmıştır. Tohum ekiminden 60 gün sonra bitkiler hasat edilmiştir. Uygulamalar yeşil aksam ve kök ağırlığını, bitki boyu ve kök uzunluğunu artırmıştır. Yaprakların klorofil içerikleri vermikompost ve sıvı yosun gübresi uygulaması ile kontrole göre artmıştır. Vermikompostun ve sıvı yosun gübresinin artan dozları rizosferdeki mikrobiyal solunum ve β-glukosidaz aktiviteyi kontrole göre önemli ölçüde artırmış, en yüksek artış vermikompost %40 ile yosun gübresinin %3’lük interaksiyonunda elde edilmiştir.

Anahtar Kelimeler

• Vermikompost
• Sıvı Yosun Gübresi
• Arpa
• β-glukosidaz
• Mikrobiyal toprak solunumu

Proje Numarası

Bu araştırmanın yapılmasında maddi destek sağlayan Harran Üniversitesi Bilimsel Araştırma Projeleri Birimine (HÜBAP-21256) teşekkürlerimizi sunarız.

Etik Beyan

“Vermikompost ve Sıvı Deniz Yosunu Ekstraktı Uygulamalarının Arpa (Hordeum vulgaris L.) Rizosferinde Mikrobiyal Solunum ve β-glukosidaz Aktiviteye Etkisi” başlıklı çalışmanın yazım sürecinde bilimsel kurallara, etik ve alıntı kurallarına uyulmuş; toplanan veriler üzerinde herhangi bir tahrifat yapılmamış ve bu çalışma herhangi başka bir akademik yayın ortamına değerlendirme için gönderilmemiştir. Bu araştırma doküman analizi ve betimsel incelemeye dayalı olarak yapıldığından etik kurul kararı zorunluluğu bulunmamaktadır.

Kaynakça

1. Adegbeye, M.J., Reddy, P.R.K., Obaisi, A.I., Elghandour, M.M.M.Y., Oyebamiji, K.J., & Salem, A.Z.M., (2019). Sustainable agriculture options for production, nutritional mitigation of greenhouse gasses and pollution, and nutrient recycling in emerging and transitional nations-an overview. Journal of Cleaner Production, 118, 319–314. https://doi.org/10.1016/j.jclepro.2019.118319
2. Anderson, J.P.E. (1982). Soil respiration. In: methods of soil analysis, part 2, chemical and microbiological properties (Ed. A.L. Page). ASA-SSSA, Madison, Winsconsin. pp. 831-871.
3. Arioli, T., Mattner, S.W., & Winberg, P.C. (2015). Applications of seaweed extracts in Australian agriculture: Past, present and future. Journal of Applied Phycology, 275, 2007-2015
4. Banerjee, S., Walder, F., Buchi, L., Meyer, M., Held, A. Y., & Gattinger, A. (2019). Agricultural intensification reduces microbial network complexity and the abundance of keystone taxa in roots. Multidisciplinary Journal of Microbial Ecology, 13, 1722–1736. https://doi.org/10.1038/s41396-019-0383-2
5. Blouin, M., Barrere, J., Meyer, N., Lartigue, S., Barot, S., & Mathieu, J. (2019). Vermicompost significantly affects plant growth. a meta-analysis. Agronomy for Sustainable Development, 39(4), 1–15. https://doi.org/10.1007/s13593-019-0579-x
6. Choudhary, V.K., & Kumar, P.S. (2019). Weed suppression, nutrient leaching, water use and yield of turmeric (Curcuma longa l.) under different land configurations and mulches. Journal of Cleaner Production, 210, 795–803. https://doi.org/10.1016/j.jclepro.2018.11.071
7. Craigie J.S. (2011). Seaweed extracts stimuli in plant science and agriculture Journal of Applied Phycology, 233, 371-393, https://doi.org/10.1007/s10811-010-9560-4
8. Demirsoy, M., & Aydın, M. (2020). The Quantitative Effects of Liquid Vermicompost and Seaweed Practices on the Seedling Quality of Organic Tomato (Solanum lycopersicum L.). International Journal of Environmental Trends (IJENT) 4, 17-27.

9. Dominguez, J. (2004). State-of-the Art and New Perspectives On Vermicomposting Research. (2nd ed), CRC Press, Boca Raton, pp. 401-424, https://doi.org/10.1201/9781420039719.ch20
10. Du Jardin, P. (2015). Plant biostimulants: definition, concept, main categories and regulation. Scientia Horticulture, 196, 3-14, https://doi.org/10.1016/j.scienta.2015.09.021
11. Gupta, R., Singh, A., Srivastava, M., Shanker, K., & Pandey, R. (2019). Plant-microbe interactions endorse growth by uplifting microbial community structure of Bacopa monnieri rhizosphere under nematode stress. Microbiological Research, 218, 87–96. https://doi.org/10.1016/j.micres.2018.10.006
12. Penuelas, J., Coello, F., & Sardans, J. (2023). A better use of fertilizers is needed for global food security and environmental sustainability. Agriculture Food Security, 12, 1-9. https://doi.org/10.1186/s40066-023-00409- 5
13. Frank, S., Havlík, P., Stehfest, E., van Meijl, H., Witzke, P., & Perez-Domínguez, I. (2019). Agricultural non-CO2 emission reduction potential in the context of the 1.5°C target. Nature Climate Change, 9, 66–72. https://doi.org/ 10.1038/s41558-018-0358-8
14. Hao, P. F., Qiu, C. W., Ding, G., Vincze, E., Zhang, G., & Zhang, Y. (2020). Agriculture organic wastes fermentation CO2 enrichment in greenhouse and the fermentation residues improve growth, yield and fruit quality in tomato. Journal of Cleaner Production, 275, 123885. https://doi.org/ 10.1016/j.jclepro.2020.123885
15. Hu, R., Liu, Y., Chen, T., Zheng, Z., Peng, G., & Zou, Y. (2021). Responses of soil aggregates, organic carbon, and crop yield to short-term intermittent deep tillage in southern China. Journal of Cleaner Production, 298, 126767. https://doi.org/10.1016/j.jclepro.2021.126767
16. Ji, R., Dong, G., Shi, W., & Min, J. (2017). Effects of liquid organic fertilizers on plant growth and rhizosphere soil characteristics of chrysanthemum. Sustainability, (9), 841-857 https://doi.org/10.3390/su9050841
17. Julia, I., Oscar, M., Analia, L., & Guilherme, J. (2020). Biofertilization with Macrocystis pyrifera algae extracts combined with PGPR enhanced growth in Lactuca sativa seedlings. Journal of Applied Phycology, (32), 4361-4371. https://doi.org/10.1007/s10811-020-02202-4
18. Khan, A.A., Jilani, G., Akhtar, M.S., Naqvi, S.M.S., & Rasheed, M. (2009). Phosphorus Solubilizing Bacteria: occurrence, Mechanisms and their Role in Crop Production. Journal of Agricultural and Biological Science, 11, 48-58
19. Kumari, R., Kaur, I., & Bhatnagar, A.K. (2011). Effect of aqueous extract of Sargassum johnstonii Setchell Gardner on growth, yield and quality of Lycopersicon esculentum Mill. Journal of Applied Phycology, 233, 623-633, https://doi.org/10.1007/s10811-011-9651-x
20. Meng, C., Gu, X., Liang, H., Wu, M., Wu, Q., Yang, L. (2022). Optimized preparation and high-efficient application of seaweed fertilizer on peanut. Journal Agriculture Research, 7, 100275. doi: 10.1016/j.jafr.2022.100275
21. Mukherjee, A., Gaurav, A.K., Patel, A.K., Singh, S., Chouhan, G.K., & Lepcha, A. (2021). Unlocking the potential plant growth-promoting properties of chickpea (Cicer arietinum l.) seed endophytes bio-inoculants for improving soil health and crop production. Land Degradation & Development, 32 (15), 4362–4374. doi: 10.1002/ldr.4042
22. Ngoroyemoto, N., Gupta, S., Kulkarni, M. G., Finnie, J. F., Van Staden, J. (2019). Effect of organic biostimulants on the growth and biochemical composition of Amaranthus hybridus. South African Journal of Botany, 124, 87–93. https://doi.org/10.1016/j.sajb.2019.03.040
23. Parastesh, F., Alikhani, H. A., & Etesami, H. (2019). Vermicompost enriched with phosphate–solubilizing bacteria provide the plant with enough phosphorus in sequential cropping under calcareous soil conditions. Journal of Cleaner Production, 221, 27–37. https://doi.org/10.1016/j.jclepro.2019.02.234
24. Raj, Y., Ali, N., Pati, A.M., & Kumar, R. (2022). Cleaner production technologies for the amelioration of soil health, biomass and secondary metabolites in Ocimum basilicum L. under Indian Western Himalaya. Frontier Plant Science, 13, 1-21. https://doi.org/10.3389/fpls.2022.976295
25. Rathore, S., & Kumar, R. (2021). Agronomic interventions affect the growth, yield, and essential oil composition of German chamomile (Matricaria chamomilla l.) in the western himalaya. Industrial Crops and Products, 171, 113873. https://doi.org/10.1016/j.indcrop.2021.113873
26. Raza, Q., Rehim, A., Bashir, M.A., Razai H.M.A., Aon, M., Geng, Y., Moustafa, M., Alshaharni, M.O., Ali, H., & Lucas, R.S. (2024). Identifying the abilities of biostimulants to improve vegetable production compared with conventional fertilizer. Frontiers in Sustainable Food Systems, 8, 1-9. https://doi.org/10.3389/fsufs.2024.1345259
27. Ruiz, J.L., & Salas, M.C. (2019). Evaluation of Organic Substrates and Microorganisms as Bio-Fertilisation Tool in Container Crop Production. Agronomy, 9(11),705, https://doi.org/10.3390/agronomy9110705
28. Sahab, S., Ibha, S.,Srivastava, V., Puneet, C., Singh, R., & Prasad, V. (2020). Potential risk assessment of soil salinity to agroecosystem sustainability: current status and management strategies. Science of the Total Environment, 764 (2020), pp. 144-164, https://doi.org/10.1016/j.scitotenv.2020.144164
29. Sarma, B., Farooq, M., Gogoi, N., Borkotoki, B., Kataki, R., & Garg, A. (2018). Soil organic carbon dynamics in wheat-green gram crop rotation amended with vermicompost and biochar in combination with inorganic fertilizers: A comparative study. Journal of Cleaner Production, 201, 471–480. https://doi.org/10.1016/j.jclepro.2018.08.004
30. Trivedi, K., Vijay Anand, K. G., Kubavat, D., Patidar, R., & Ghosh, A. (2018). Drought alleviatory potential of Kappaphycus seaweed extract and the role of the quaternary ammonium compounds as its constituents towards imparting drought tolerance in Zea mays. Journal of Applied Phycology, 30 (3), 2001–2015. https://doi.org/ 10.1007/s10811-017-1375-0
31. Trivedi, K., Kumar, R., Vijay Anand, K. G., Bhojani, G., Kubavat, D., & Ghosh, A. (2022). Structural and functional changes in soil bacterial communities by drifting spray application of a commercial red seaweed extract as revealed by metagenomics. Archives of Microbiology, 204 (1), 1–21. https://doi.org/10.1007/s00203-021- 02644-5
32. Yasmin, S., & D'Souza, D. (2007). Effect of pesticides on the reproductive output of Eisenia fetida. The Bulletin of EnvironmentalContamination and Toxicology, 795, 529-532, https://doi.org/110.1007/s00128-007-9269-5
33. Voko, M.P., Kulkarni, M., Ngoroyemoto, N., Gupta, S., Finnie, J., & van Staden, J. (2022). Vermicompost leachate, seaweed extract and smoke-water alleviate drought stress in cowpea by influencing phytochemicals, compatible solutes and photosynthetic pigments. Plant Growth Regulation. 97, 327-342 https://doi.org/10.1007/s10725-022-00815-y
34. Zhang, Q., Song, Y., Wu, Z., Yan, X., Gunina, A., & Kuzyakov, Y. (2020). Effects of six-year biochar amendment on soil aggregation, crop growth, and nitrogen and phosphorus use efficiencies in a rice-wheat rotation. Journal of Cleaner Production. 242, 118435. https://doi.org/10.1016/j.jclepro.2019.118435