Changes in Cytokine Levels and Oxidative Status Against Coxiella burnetii Infection in Aborted Sheep

Öz

The aim of this study was to investigate the levels of nuclear factor kappa-B (NF-B), malondialdehyde (MDA), glutathione (GSH), interleukin 1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) against naturally acquired Coxiella burnetii (C.burnetii) infection in abortive sheep. The sera of aborted (n = 20) and healthy (n = 20) sheep were used in the study. The enzyme-linked immunosorbent assay (ELISA) was used to detect host antibodies against C. burnetii. The MDA was higher in C. burnetii-positive sheep than in healthy group (p < 0.001). GSH level were significantly lower in C. burnetii-positive sheep compared with the healthy sheep. The cytokine levels were statistically higher in C. burnetii-positive sheep than in healthy sheep (p < 0.001). A positive correlation was found between MDA, NF-B, TNF-α, and IL-1β levels and a negative correlation between GSH level (p < 0.01). In addition, a strong negative correlation was observed between the levels of cytokines and antioxidants (p < 0.01). In conclusion, oxidative stress and cytokine markers may serve as valuable diagnostic and prognostic tools for C. burnetii infection in sheep.

Anahtar Kelimeler

• Abortion
• C. burnetii
• cytokine
• oxidative stress
• sheep

Abort Yapan Koyunlarda Coxiella burnetii Enfeksiyonuna Karşı Sitokin Düzeyleri ve Oksidatif Durumdaki Değişiklikler

Öz

Bu çalışmanın amacı, abort yapan koyunlarda doğal olarak edinilmiş Coxiella burnetii (C.burnetii) enfeksiyonuna karşı nükleer faktör kappa-B (NF-B), malondialdehit (MDA), glutatyon (GSH) ve interlökin 1β (IL-1β) ve tümör nekroz faktörü-alfa (TNF-α) düzeylerini araştırmaktır. Çalışmada abortuslu (n=20) ve sağlıklı (n=20) koyunların serumları kullanıldı. C. burnetii’ye karşı konak antikorlarını tespit etmek için enzim bağlı immünosorbent testi (ELISA) kullanıldı. MDA, C. burnetii pozitif koyunlarda sağlıklı gruba göre daha yüksekti (p<0,001). GSH düzeyi, C. burnetii pozitif koyunlarda sağlıklı koyunlara göre anlamlı derecede düşüktü. Sitokin düzeyleri C. burnetii pozitif koyunlarda sağlıklı koyunlara göre istatistiksel olarak daha yüksekti (p < 0,001). MDA, NF-B, TNF-α ve IL-1β düzeyleri arasında pozitif bir korelasyon ve GSH düzeyi arasında negatif bir korelasyon bulundu (p < 0,01). Ek olarak, sitokin ve antioksidan düzeyleri arasında güçlü bir negatif korelasyon gözlendi (p < 0,01). Sonuç olarak, oksidatif stres ve sitokin belirteçleri koyunlarda C. burnetii enfeksiyonunun prognoz-tanısı için ek araçlar olarak kullanılabilme potansiyeli vardır.

Anahtar Kelimeler

• Abort
• C. burnetii
• sitokin
• oksidatif stres
• koyun

Kaynakça

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