Efficacy of Electrolysed Saline Water Against Multidrug-Resistant Staphylococcus pseudintermedius in a Canine Postoperative Pin Tract Infection: An in-situ Study With In Vitro and Clinical Validation

Yıl 2025, Cilt: 15 Sayı: 2, 217 - 223, 25.12.2025

Ali Kumandaş , Mitat Şahin , Ayperi Aytmirzakızı , Aiturgan Duyshobaeva , Ege Toprak

https://doi.org/10.53518/mjavl.1721237

https://izlik.org/JA89NX87ZD

Öz

This study evaluated the antimicrobial efficacy of electrolysed saline water (pH 8.5) against Staphylococcus pseudintermedius isolated purely from a pin tract infection in a dog following orthopaedic surgery. The main material of the study was a 4-month-old female stray dog. An old antebrachium fracture was identified in the right foreleg and pinhole infection and fistula were detected 17 days after osteosynthesis. After the swab sample was taken from the wound, the pin was removed and the wound was irrigated with electrolyzed saline.The isolate obtained from the wound exhibited multidrug resistance to penicillin, trimethoprim-sulfamethoxazole, and tetracycline but remained susceptible to oxacillin, enrofloxacin, and vancomycin. Macro dilution testing revealed a log₁₀ reduction of 4.4 in S. pseudintermedius viability upon exposure to electrolysed water. Clinical application involving daily systemic ceftriaxone and topical irrigation with electrolysed water demonstrated synergistic effects, resolving the infection within one week. Follow-up examination confirmed complete wound closure, cessation of discharge, and no bacterial growth in swab cultures. These findings suggest that electrolysed water is an effective adjunct therapy for biofilm-associated surgical site infections, offering advantages in antimicrobial stewardship and zoonotic risk mitigation.

Anahtar Kelimeler

Antimicrobial resistance , biofilm , electrolysed water , S. pseudintermedius , veterinary surgery

Etik Beyan

Under de Article (8) k of Turkey’s transposed legislation (Reg. 28914) implementing Directive 2010/63/EU, clinical veterinary practices performed exclusively for patient care are exempt from ethical evaluation. An informed consent form was obtained from the animal owner.

Destekleyen Kurum

This study was not supported by any institution.

Bir Köpekte Postoperatif Pin Yolu Enfeksiyonunda Çoklu İlaç Dirençli Staphylococcus pseudintermedius'a Karşı Elektrolize Tuzlu Suyun Etkinliği: In Vitro ve Klinik Validasyon ile Desteklenen İn-Situ Araştırma

https://izlik.org/JA89NX87ZD

Öz

Bu çalışmada, ortopedik cerrahi sonrası bir köpekte pin deliği enfeksiyonundan saf olarak izole edilen Staphylococcus pseudintermedius'a karşı elektrolize tuzlu suyun (pH 8.5) antimikrobiyal etkinliği değerlendirilmiştir. Çalışmanın ana materyalini 4 aylık dişi bir sokak köpeği oluşturdu. Sağ ön bacakta eski bir antebrachium kırığı belirlendi ve osteosentezden sonraki 17. günde pin deliği enfeksiyonu ve fistül tespit edildi. Yaradan alınan swap örneği sonrası pin çıkartılarak elektrolize tuzlu su ile yaranın irrigasyonu yapıldı. Yaradan elde edilen izolat penisilin, trimetoprim-sülfametoksazol ve tetrasikline çoklu ilaç direnci gösterirken, oksasilin, enrofloksasin ve vankomisine duyarlılık göstermiştir. Elektrolize suyun makrodilüsyon testi ile antmikrobiyal aktivitesi ölçüldüğünde S.pseudintermedius için minimum inhibitör konsantrasyon değerinin log10 redüksiyonunun 4,4 olduğunu ortaya koymuştur. Günlük topikal elektrolize su irrigasyonu ve sistemik seftriakson ile klinik uygulamada, enfeksiyonu bir hafta içinde engelleyerek sinerjik etkiler gösterdiği ortaya konulmuştur. Bir hafta sonraki kontrolde yaranın tamamen kapandığı akıntının kesildiği ve swap ile alınan örnekte Staphylococcus pseudintermedius’un üremediği görülmüştür. Bu bulgular, elektrolize suyun biyofilm ilişkili cerrahi alan enfeksiyonları için etkili bir yardımcı tedavi olduğunu, antimikrobiyal yönetim ve zoonotik risk azaltmada avantajlar sunduğunu göstermektedir. çeşit olmuştur.

Anahtar Kelimeler

Antimikrobiyal direnç , biyofilm , elektrolize su , S. pseudintermedius , veteriner cerrahi

Etik Beyan

Türkiye'nin 2010/63/AB Direktifini uygulayan aktarılmış mevzuatının (Yönetmelik 28914) (8) k maddesi uyarınca, yalnızca hasta bakımı için gerçekleştirilen klinik veterinerlik uygulamaları etik değerlendirmeden muaftır. Hayvan sahibinden bilgilendirilmiş onam formu alındı.

Destekleyen Kurum

Bu çalışma herhangi bir kurum tarafından desteklenmemiştir.

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