Deksametazon Uygulanan Ratlarda Alendronat ve Vitamin D’nin Hematolojik Parametreler Üzerıne Etkisi

Öz

Deksametazon, bağışıklık yanıtını baskılaması ve sitokin üretimini düşürmesiyle tedavi amacıyla sıklıkla tercih edilen, güçlü immünsüpresif ve anti-inflamatuvar etkiye sahip sentetik bir glukokortikoid türevidir. Bu çalışma ile ratlarda deksametazon kaynaklı osteoporotik etkilerin tedavisinde sıkça kullanılan alendronat ve vitamin D’nin hematolojik parametrelere etkileri araştırıldı. Bu amaçla 6 hafta sürdürülen çalışma için toplam 35 adet rat 5 gruba ayrıldı. Kontrol grubuna herhangi bir uygulama yapılmazken, diğer tüm gruplara deksametazon (7.5 mg/kg haftada bir, deri altı) verildi. Üç tedavi grubuna Alendronat (haftada 5 gün 0.9 mg/kg), Vitamin D (haftada 1 kez 10.000UI/500µL) ve Alendronat+Vitamin D uygulandı. Sonuçta tüm gruplarda WBC ve LYM düzeylerinin kontrol grubuna göre anlamlı derecede düşük olduğu tespit edildi. Alendronat grubu dışında tüm gruplarda anlamlı olmak üzere RBC sayılarının daha düşük, RDWCV ve RDWSD’nin ise daha yüksek olduğu gözlendi. Deksametazon grubunda kontrollere göre HGB ve MCHC düzeyleri ise anlamlı derecede düşük, MCV ise yüksek bulundu. Tüm tedavi gruplarında kontrol ve deksametazon grubuna göre anlamlı derecede daha düşük bulunan PLT ve PCT’deki azalmanın alendronat ve vitamin D’nin birlikte kullanımıyla belirginleştiği görüldü. Sonuç olarak, bu çalışma ile belirlenen dozlarda deksametazonun özellikle lökosit ve eritrosit indeksleri olmak üzere çeşitli hematolojik etkilerinin düzenlenmesinde alendronat ve vitamin D’nin kullanılan doz ve sürelerde sınırlı etkiye sahip oldukları gözlendi.

Anahtar Kelimeler

• Alendronat
• D vitamini
• Deksametazon
• Hematolojik parametreler
• Rat

Etik Beyan

Selçuk Üniversitesi Deneysel Tıp Araştırma ve Uygulama Merkezi'nde (SÜDAM), 30.12.2025 tarih ve 2025-120 sayılı etik onayı alınarak gerçekleştirilmiştir.

The Effect of Alendronate and Vitamin D on Hematological Parameters in Rats Administered Dexamethasone

Öz

Dexamethasone is a synthetic glucocorticoid derivative with potent immunosuppressive and anti-inflammatory effects that is frequently used for therapeutic purposes due to its ability to suppress the immune response and reduce cytokine production. This study investigated the effects of alendronate and vitamin D commonly used to treat dexamethasone-induced osteoporotic effects in rats on hematological parameters. For this 6-week study, a total of 35 rats were divided into 5 groups. While no treatment was administered to the control group, all other groups received dexamethasone (7.5 mg/kg once weekly, subcutaneously). Three treatment groups received alendronate (0.9 mg/kg for 5 days per week), vitamin D (10,000 IU/500 µL once weekly), and alendronate + vitamin D. As a result, it was found that WBC and LYM levels were significantly lower in all groups compared to the control group. Except for the alendronate group, significantly lower RBC counts and higher RDWCV and RDWSD values were observed in all groups. In the dexamethasone group, HGB and MCHC levels were significantly lower than in the controls, while MCV was higher. The decrease in PLT and PCT, which was significantly lower in all treatment groups compared to the control and dexamethasone groups, was found to be more pronounced with the combined use of alendronate and vitamin D. In conclusion, this study found that alendronate and vitamin D had only a limited effect on regulating the various hematological effects of dexamethasone particularly on leukocyte and erythrocyte indices at the doses and durations used.

Anahtar Kelimeler

• Alendronate
• Vitamin D
• Dexamethasone
• Hematological parameters
• Rat

Etik Beyan

This study was conducted at the Selçuk University Center for Experimental Medicine Research and Application (SÜDAM) following the receipt of ethical approval dated December 30, 2025, and numbered 2025-120.

Kaynakça

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