Investigation of the CAST Gene Polymorphism in Karayaka Sheep

Öz

 The study aimed to investigate the CAST gene's genetic polymorphism, known as a candidate gene for meat quality and quantities, using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method in Karayaka sheep. The blood samples were collected from 105 animals belong to four subpopulations (Samsun, Ordu, Giresun, and Tokat) in Turkey’s Black Sea region provinces. The genomic DNA was isolated by using an extraction kit. A fragment of 622 bp on CAST gene was amplified by PCR and then genotypes of the CAST gene for all individuals were determined with the restriction endonuclease MspI. At the end of the work, MM and MN’s genotypic frequencies were found to be 84 and 16% for the Karayaka breed. While the MN genotype was not observed only in the Ordu subpopulation, the NN genotype was not observed in any subpopulations studied. As a result, the obtained findings provided information about the CAST gene polymorphism for Karayaka breed at the population level. This result could be considered for genomic selection works to improve the meat quality traits of Karayaka in the future.

Anahtar Kelimeler

• Karayaka sheep
• calpastatin gene
• genetic variability
• PCR-RFLP
• MspI

Proje Numarası

yok

Kaynakça

1. Aksoy Y, Çiçek Ü, Şen U, Şirin E, Uğurlu M, Önenç A, Kuran M, Ulutaş, Z. 2019. Meat production characteristics of Turkish native breeds: II. meat quality, fatty acid, and cholesterol profile of lambs. Archives Animal Breeding. 62 (1): 41.
2. Ardicli S, Dincel D, Samli H, Balci F. 2017. Effects of polymorphisms at LEP, CAST,CAPN1,GHR,FABP4 and DGAT1 genes on fattening performance and carcass traits in Simmental bulls. Archiv fuer Tierzucht. 60 (2): 61.
3. Armstrong E, Ciappesoni G, Iriarte W, Da Silva C, Macedo F, Navajas EA, Brito G, Julian S, Gimeno D, Postiglioni, A. 2018. Novel genetic polymorphisms associated with carcass traits in grazing Texel sheep. Meat science 145: 202-208.
4. Avanus, K., Genetic Variability of CAST Gene in Native Sheep Breeds of Turkey. 2015. Kafkas Universitesi Veteriner Fakultesi Dergisi. 21 (6): 789-794.
5. Balcıoğlu MS, Karslı T, Șahin E, Ulutaș Z, Aksoy Y 2014. Determination of calpastatin (CAST) gene polimorphism in some native sheep breeds reared in Turkey by PCR-RFLP method. Tarim Bilimleri Dergisi. 20 (4): 427-433.
6. Bayram D, Akyüz B, Arslan K, Özdemir F, Aksel EG, Çinar MU 2019 . DGAT1, CAST and IGF-I Gene Polymorphisms in Akkaraman Lambs and Their Effects on Live Weights up to Weaning Age. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 25(1): 9-15.
7. Bozhilova-Sakova, M, Dimitrova I, Tzonev TI, Petrov N. 2020. Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method. Bulgarian Journal of Agricultural Science. 26 (2): 475-479.
8. Cam MA, Olfaz M, Garipoglu AV. 2007. Shearing male lambs in the cold season improves the carcass yield without affecting fattening performance. Animal science journal. 78 (3): 259-265.

9. Charlesworth B. Effective population size and patterns of molecular evolution and variation. Nature Reviews Genetics. 10(3): 195.
10. Chung H, Davis M. 2012. PCR-RFLP of the ovine calpastatin gene and itsassociation with growth. Asian J Anim Vet Adv. 7(8): 641-652.
11. Dincel D, Ardicli S, Soyudal B, Er M, Alpay F, ŞAMLI H, Balci F. 2015. Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep. Kafkas Universitesi Veteriner Fakultesi Dergisi. 21 (4): 483-488.
12. Donicer Montes V, Claudia Lenis V, Darwin Hernández H. 2019. Polymorphisms of the calpain and calpastatin genes in two populations of colombian creole sheep. Revista MVZ Córdoba. 24(1): 7113-7118.
13. Eftekhari Shahroudi F, Nassiry MR, Valizadh R, Heravi Moussavi A, Tahmoores Pour M, Ghiasi H. 2006. Genetic polymorphism at MTNR1A, CAST and CAPN loci in Iranian Karakul sheep. Iranian Journal of Biotechnology. 4 (2): 117-122.22.
14. Gabor M, Trakovická A, Miluchová M. 2009. Analysis of polymorphism of CAST gene and CLPG gene in sheep by PCR-RFLP method. Scientific Papers Animal Science and Biotechnologies. 42 (2): 470-476.
15. Gabor, M., TRAKOVICKÁ, A., & MILUCHOVÁ, M. (2009). Analysis of polymorphism of CAST gene and CLPG gene in sheep by PCR-RFLP method. Scientific Papers Animal Science and Biotechnologies, 42(2), 470-476.
16. Gao Y U, Zhang R, Hu X, Li N. 2007. Application of genomic technologies to the improvement of meat quality of farm animals. Meat science. 77 (1): 36-45.
17. Gharahveysi S, Abbasi HA, Irani M, Abdullahpour R, Mirhabibi S. 2012. Polymorphism investigation of calpastatin gene in Zel sheep population of Iran by PCR-RFLP method. African Journal of Biotechnology. 11(13): 3211-3214.
18. Greguła-Kania M, Gruszecki TM, Junkuszew A, Juszczuk-Kubiak E, Florek M. 2019. Association of CAST gene polymorphism with carcass value and meat quality in two synthetic lines of sheep. Meat science. 154: 69-74.
19. Grochowska E, Borys B, Lisiak D, Mroczkowski S. 2019. Genotypic and allelic effects of the myostatin gene (MSTN) on carcass, meat quality, and biometric traits in Colored Polish Merino sheep. Meat science. 151; 4-17.
20. Khan S, Riaz MN, Ghaffar A, Ullah MF. 2012. Calpastatin (CAST) gene polymorphism and its association with average daily weight gain in Balkhi and Kajli sheep and Beetal goat breeds. Pakistan J Zool. 44 (2): 377-382, 2012.
21. Khederzadeh S. 2011. Polymorphism of calpastatin gene in crossbreedDalagh sheep using PCR-RFLP. Afr J Biotechnol. 10 (53): 10839-10841.
22. Kulikova K, Yuldashbaev Y, Hatataev S. 2018. The polymorphism of Cast and GDF9 genes in the Tuvan short-fat-tailed sheep population. Scientific Papers-Series D-Animal Science. 61 (1):14-17.
23. Kumar SN, Jayashankar MR, Chandrashekhar M, Vageesh P, Nagaraja R, Maniu GU. 2016. Association of genetic polymorphisms of Calpain and Calpastatin genes with growth traits in Bandur sheep. Indian Journal of Animal Sciences. 86 (8): 907-912.
24. Nanekarani S, Asadi N, Khederzadeh S. 2011. Genotypic frequency of Calpastatin gene in Lori sheep by PCR-RFLP method. Anim. Sci. 72: 606-620. Nikmard, M, Molaee V, Eskandarinasab MP, Dinparast Djadid N, Vajhi AR. 2012. Calpastatin polymorphism in Afshari sheep and its possible correlation with growth and carcass traits. Journal of Applied Animal Research. 40 (4): 346-350.
25. Oğan, M. 2000. Effects of shearing on fattening performance of Karayaka male lambs. Veteriner Fakültesi Dergisi, Uludağ Üniversitesi. 19 (1/2): 25-27.
26. Olfaz M, Ocak N, Erener G, Cam MA, Garipoglu A V. 2005. Growth, carcass and meat characteristics of Karayaka growing rams fed sugar beet pulp, partially substituting for grass hay as forage. Meat Science. 70 (1): 7-14.
27. Palmer BR, Roberts N, Hickford JG, Bickerstaffe R. 1998. Rapid communication: PCR-RFLP for MspI and NcoI in the ovine calpastatin gene. J. Anim. Sci. 76 (5): 1499-1500.
28. Pomitun I, Rossokha V, Boyko Y, Guzevatyi O, Shpilka M, Kulibaba R. 2019. Analysis of calpastatin and сallipyge genes polymorphism in Prydniprovska meat sheep. Agricultural Science and Practice. 6 (2): 58-65.
29. Slatkin M. 1987. Gene flow and the geographic structure of natural populations. Science. 236(4803):787-792. Suleman, M., et al., Calpastatin (CAST) gene polymorphism in Kajli, Lohi and Thalli sheep breeds. African Journal of Biotechnology, 2012. 11(47): p. 10655-10660. Sun Y, Luo W, Xie H, Zhang Y, Cai H. 2015. Analysis of association between polymorphism of TFB2M gene and meat quality, growth and slaughter traits in Guizhou White Goat, a well-known Chinese indigenous goat breed. Pakistan Journal of Zoology. 47 (6): 1605-1610.
30. Szkudlarek-Kowalczyk, ME. 2011. Wiśniewska, and S. Mroczkowski, Polymorphisms of calpastatin gene in sheep. Journal of Central European Agriculture. 12(3): 425-432.
31. Troell K, Engström A, Morrison DA, Mattsson JG, Höglund J. 2006. Global patterns reveal strong population structure in Haemonchus contortus, a nematode parasite of domesticated ruminants. International journal for parasitology. 36 (12): 1305-1316.
32. TUIK (TurkishStatistical Institute), available at: http://www.tuik.gov.tr/UstMenu.do?metod=temelist(last access: 13 July 2020), 2019. 
33. Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM. PopGene32, Microsoft Windows-based freeware for population genetic analysis, version 1.32. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Alberta, Canada, 2000.
34. Yilmaz O, Cemal I, Karaca O, Ata N 2014b. Association of Calpastatin (CAST) gene polymorphism with weaning weight and ultrasonic measurements of loin eye muscle in Kıvırcık lambs. Journal of the Faculty of VeterinaryMedicine, Kafkas University. 20 (5): 675-680.
35. Yilmaz O, Sezenler T, Ata N, Yaman Y, Cemal I, Karaca O. 2014a. Polymorphism of the ovine calpastatin gene in some Turkish sheep breeds. Turkish Journal of Veterinary and Animal Sciences, 38(4), 354-357.
36. Zhang CY, Wang Z, Bruce HL, Janz J, Goddard E, Moore S, Plastow GS. 2014. Associations between single nucleotide polymorphisms in 33 candidate genes and meat quality traits in commercial pigs. Animal genetics. 45 (4): 508-516.