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Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem

Yıl 2015, Cilt: 5 Sayı: 1, 20 - 31, 01.06.2015

Öz

We compared a microbial diversity in uncontaminated and contaminated with petroleum soils in mountain ecosystems in Kyrgyzstan. Culture-dependent and culture - independent methods were used to analyze microbial diversity. PCR primers that target conserved iron binding motifs in alkane monooxygenase (alkB) and cytochrome P450 alkane hydroxylase were used to analyze genes of alkane degrading bacteria in chronically contaminated and uncontaminated sites, using an enrichment culture with dodecane. Analysis of 16S rRNA showed that in contaminated soils BetaProteobacteria phylum populations were abundant. In uncontaminated soil Gammaproteobacteria phylum populations were abundant. The Shannon index (H′) was used to estimate the microdiversity in soils. Diversity was higher in the uncontaminated (H′ = 4,234) than in the contaminated (H′ = 3.632) soil biotopes. These studies have shown that oil contamination affects the structure of local soil microbiota, undermines biodiversity and increases the activity of bacteria involved in hydrocarbon degradation, for example Proteobacteria phylum populations.

Kaynakça

  • [1] Allard A.S, Neilson A.H.( 1997). Bioremediation of organic waste sites: a critical review of microbiological aspects // Int. Biodeterioration. V. 39. P. 253-285.
  • [2] Atlas R.M, Bartha R. (1992). Hyrdocarbon biodegradation and oil spill bioremediation //Adv. Microb Eco.. V. 12. P. 287- 338.
  • [3] Master E.R, Mohn W.W.(2001). Induction of bphA, encoding biphenyl dioxygenase,in two polychlorinated biphenyl- degrading bacteria, psychrotolerant Pseudomonas strain Cam-1 and mesophilic Burkholderi.
  • [4] Atlas R.M.( 1981). Microbial degradation of petroleum hydrocarbons: an environmental perspective // Microbiol Rev. V. 45. P.180 -209.
  • [5] Van Elsas J. D, Trevors J.T.( 1997).Modern Soil Microbiology. New York: Marcel Dekker.
  • [6] Abed R.M.M., Safi N.M.D., Köster J., de Beer D., El-Nahhal Y., Rullkötter J., García-Pichel F. (2002). Microbial diversity of a heavily polluted microbial mat and its community changes following degradation of petroleum compounds// Appl. Environ Microbiol. V. 68.P.1674-1683.
  • [7] Chaillan F., Le Flèche A., Bury E., Phantavong Y., Grimont P., Saliot A., Oudot J. ( 2004). Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms // Res Microbiol. V. 155. P. 587-595.
  • [ 8] Chaineau C. H., Morel J., Dupont J., Bury E., Outdot J. ( 1999). Comparison of the fuel oil biodegradation potential ofhydrocarbon- assimilating microorganisms isolated from a temperate agricultural soil //Sci.Total Environ. V.227.P. 237-247.
  • [9] Dojka M.A, Hugenholtz P, Haack S.K, Pace N.R.(1998). Microbial diversity in a hydrocarbon and chlorinatedsolvent-contaminated aquifer undergoing intrinsic bioremediation // Appl. Environ Microbiol.V.64. P. 3869-3877.
  • [10] Macnaughton S.J., Stephen J.R., Venosa A.D., Davis G.A. , Chang Y., White D.C .(1999). Microbial population changes during bioremediation of an experimental oil spill // Appl. Environ Microbiol, 65: 3566-3574.
  • [11] Radwan S.S., Sorkhoh N.A., Fardoun F., Al-Hasan R.H .( 1995). Soil management enhancing hydrocarbon biodegradation in the polluted Kuwaiti desert //Appl.MicrobiolBiotechnol. 44: 265-270.
  • [12] Rahman K.S., Rahman T., Lakshmanaperumalsamy P., Banat I.M. (2002). Occurrence of crude oil degrading bacteria in gasoline and diesel station soils// J. Basic Microbiol. 42 : 284-291.
  • [13] Chaillan F., Le Flèche A., Bury E., Phantavong Y., Grimont P., Saliot A., Oudot J. ( 2004). Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms //. Res Microbiol. 155: 587- 595.
  • [14] Haddock J.D, Gibson D. T.(1995). Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400 // J. Bacteriol. 177: 5834-5843.
  • [15] Kim, D., Y.S. Kim., S.K. Kim., S. W. Kim., G. J. Zylstra. (2002). Monocyclic aromatic hydrocarbon degradation by Rhodococcus sp. strain DK17 // Appl. Environ. Microbiol. 68: 3270-3278.
  • [16] Tamura K., Dudley J., Nei M., Kumar S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 // Mol. Biol. Evol. 24(8): 1596–1599.
  • [17] Hurlbert S.H.(1971). The nonconcept of species diversity: a critique and alternative parameters// Ecology, 1971; 52: 577-586.
  • [18] Hill M.O. (1973). Diversity and evenness: a unifying notion and its consequences.
  • [19] Hill T.C, Walsh K.A, Harris J.A., Moffett B.F. (2003). Using ecological diversity measures with bacterial communities // FEMS Microbiology Ecology, 43: 1-10.
  • [20] Chao A.(1984). Nonparametric estimation of the number of classes in a population. Scandinavian J. Stat. 11: 265-270.
  • [21] Pearson A., Kraunz K. S., Sessions A.L, Dekas A.E, Leavitt W.D, Edwards K.J.( 2008). Quantifying microbial utilization of petroleum hydrocarbons in salt marsh sediments by using the 13C content of bacterial rRNA// Appl. Environ Microbiol. 74: 1157-1166.
  • [22] Viсas M, Sabat J, Espuny M.J, Solanas A.M. ( 2005). Bacterial сommunity dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote- contaminated soil// Appl. Environ Microbiol.71: 7008-7018.
  • [23] Wawrik B., Kerkhof L., Kukor J, Zylstra G. ( 2005). Effect of different carbon sources on community composition of bacterial enrichments from soil//Appl. Environ. Microbiol. 71: 6776- 6783.
  • [24] Margesin R, Schinner F. (1997). Laboratory bioremediation experiments with soil from a diesel –oil contaminated site –significant role of cold–adapted microorganisms and fertilizers// Chem. Technol. Biotechnol. 70: 92-98.
  • [25] Margesin R, Schinner F. ( 1997a). Bioremediation of diesel-oil –contaminated alpine soils at low temperatures //Appl. Microbiol. Boitechnol. 47: 462-468.
  • [26] Margesin R, Schinner F. (1998). Oil biodegradation potential in alpine habitats// Arctic Alpine Res. 30: 262-265
Yıl 2015, Cilt: 5 Sayı: 1, 20 - 31, 01.06.2015

Öz

Kaynakça

  • [1] Allard A.S, Neilson A.H.( 1997). Bioremediation of organic waste sites: a critical review of microbiological aspects // Int. Biodeterioration. V. 39. P. 253-285.
  • [2] Atlas R.M, Bartha R. (1992). Hyrdocarbon biodegradation and oil spill bioremediation //Adv. Microb Eco.. V. 12. P. 287- 338.
  • [3] Master E.R, Mohn W.W.(2001). Induction of bphA, encoding biphenyl dioxygenase,in two polychlorinated biphenyl- degrading bacteria, psychrotolerant Pseudomonas strain Cam-1 and mesophilic Burkholderi.
  • [4] Atlas R.M.( 1981). Microbial degradation of petroleum hydrocarbons: an environmental perspective // Microbiol Rev. V. 45. P.180 -209.
  • [5] Van Elsas J. D, Trevors J.T.( 1997).Modern Soil Microbiology. New York: Marcel Dekker.
  • [6] Abed R.M.M., Safi N.M.D., Köster J., de Beer D., El-Nahhal Y., Rullkötter J., García-Pichel F. (2002). Microbial diversity of a heavily polluted microbial mat and its community changes following degradation of petroleum compounds// Appl. Environ Microbiol. V. 68.P.1674-1683.
  • [7] Chaillan F., Le Flèche A., Bury E., Phantavong Y., Grimont P., Saliot A., Oudot J. ( 2004). Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms // Res Microbiol. V. 155. P. 587-595.
  • [ 8] Chaineau C. H., Morel J., Dupont J., Bury E., Outdot J. ( 1999). Comparison of the fuel oil biodegradation potential ofhydrocarbon- assimilating microorganisms isolated from a temperate agricultural soil //Sci.Total Environ. V.227.P. 237-247.
  • [9] Dojka M.A, Hugenholtz P, Haack S.K, Pace N.R.(1998). Microbial diversity in a hydrocarbon and chlorinatedsolvent-contaminated aquifer undergoing intrinsic bioremediation // Appl. Environ Microbiol.V.64. P. 3869-3877.
  • [10] Macnaughton S.J., Stephen J.R., Venosa A.D., Davis G.A. , Chang Y., White D.C .(1999). Microbial population changes during bioremediation of an experimental oil spill // Appl. Environ Microbiol, 65: 3566-3574.
  • [11] Radwan S.S., Sorkhoh N.A., Fardoun F., Al-Hasan R.H .( 1995). Soil management enhancing hydrocarbon biodegradation in the polluted Kuwaiti desert //Appl.MicrobiolBiotechnol. 44: 265-270.
  • [12] Rahman K.S., Rahman T., Lakshmanaperumalsamy P., Banat I.M. (2002). Occurrence of crude oil degrading bacteria in gasoline and diesel station soils// J. Basic Microbiol. 42 : 284-291.
  • [13] Chaillan F., Le Flèche A., Bury E., Phantavong Y., Grimont P., Saliot A., Oudot J. ( 2004). Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms //. Res Microbiol. 155: 587- 595.
  • [14] Haddock J.D, Gibson D. T.(1995). Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400 // J. Bacteriol. 177: 5834-5843.
  • [15] Kim, D., Y.S. Kim., S.K. Kim., S. W. Kim., G. J. Zylstra. (2002). Monocyclic aromatic hydrocarbon degradation by Rhodococcus sp. strain DK17 // Appl. Environ. Microbiol. 68: 3270-3278.
  • [16] Tamura K., Dudley J., Nei M., Kumar S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 // Mol. Biol. Evol. 24(8): 1596–1599.
  • [17] Hurlbert S.H.(1971). The nonconcept of species diversity: a critique and alternative parameters// Ecology, 1971; 52: 577-586.
  • [18] Hill M.O. (1973). Diversity and evenness: a unifying notion and its consequences.
  • [19] Hill T.C, Walsh K.A, Harris J.A., Moffett B.F. (2003). Using ecological diversity measures with bacterial communities // FEMS Microbiology Ecology, 43: 1-10.
  • [20] Chao A.(1984). Nonparametric estimation of the number of classes in a population. Scandinavian J. Stat. 11: 265-270.
  • [21] Pearson A., Kraunz K. S., Sessions A.L, Dekas A.E, Leavitt W.D, Edwards K.J.( 2008). Quantifying microbial utilization of petroleum hydrocarbons in salt marsh sediments by using the 13C content of bacterial rRNA// Appl. Environ Microbiol. 74: 1157-1166.
  • [22] Viсas M, Sabat J, Espuny M.J, Solanas A.M. ( 2005). Bacterial сommunity dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote- contaminated soil// Appl. Environ Microbiol.71: 7008-7018.
  • [23] Wawrik B., Kerkhof L., Kukor J, Zylstra G. ( 2005). Effect of different carbon sources on community composition of bacterial enrichments from soil//Appl. Environ. Microbiol. 71: 6776- 6783.
  • [24] Margesin R, Schinner F. (1997). Laboratory bioremediation experiments with soil from a diesel –oil contaminated site –significant role of cold–adapted microorganisms and fertilizers// Chem. Technol. Biotechnol. 70: 92-98.
  • [25] Margesin R, Schinner F. ( 1997a). Bioremediation of diesel-oil –contaminated alpine soils at low temperatures //Appl. Microbiol. Boitechnol. 47: 462-468.
  • [26] Margesin R, Schinner F. (1998). Oil biodegradation potential in alpine habitats// Arctic Alpine Res. 30: 262-265
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA32PV84ZP
Bölüm Araştırma Makalesi
Yazarlar

Tinatin Döölotkeldieva Bu kişi benim

Saykal Bobuşeva Bu kişi benim

Maxabat Konurbaeva Bu kişi benim

Yayımlanma Tarihi 1 Haziran 2015
Gönderilme Tarihi 1 Haziran 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 5 Sayı: 1

Kaynak Göster

APA Döölotkeldieva, T., Bobuşeva, S., & Konurbaeva, M. (2015). Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem. Manas Journal of Agriculture Veterinary and Life Sciences, 5(1), 20-31.
AMA Döölotkeldieva T, Bobuşeva S, Konurbaeva M. Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem. MJAVL. Haziran 2015;5(1):20-31.
Chicago Döölotkeldieva, Tinatin, Saykal Bobuşeva, ve Maxabat Konurbaeva. “Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem”. Manas Journal of Agriculture Veterinary and Life Sciences 5, sy. 1 (Haziran 2015): 20-31.
EndNote Döölotkeldieva T, Bobuşeva S, Konurbaeva M (01 Haziran 2015) Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem. Manas Journal of Agriculture Veterinary and Life Sciences 5 1 20–31.
IEEE T. Döölotkeldieva, S. Bobuşeva, ve M. Konurbaeva, “Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem”, MJAVL, c. 5, sy. 1, ss. 20–31, 2015.
ISNAD Döölotkeldieva, Tinatin vd. “Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem”. Manas Journal of Agriculture Veterinary and Life Sciences 5/1 (Haziran 2015), 20-31.
JAMA Döölotkeldieva T, Bobuşeva S, Konurbaeva M. Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem. MJAVL. 2015;5:20–31.
MLA Döölotkeldieva, Tinatin vd. “Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem”. Manas Journal of Agriculture Veterinary and Life Sciences, c. 5, sy. 1, 2015, ss. 20-31.
Vancouver Döölotkeldieva T, Bobuşeva S, Konurbaeva M. Bacterial Community Structure in Petroleum Contaminated and Uncontaminated Soils of Mountain Ecosystem. MJAVL. 2015;5(1):20-31.