Ankaferd® Kanama Durdurucunun Beyin Parankiminde Hemostatik Etkisi: Deneysel Çalışma

Yıl 2022, , 31 - 37, 28.04.2022

Emrah Egemen , Ümit Akın Dere , Başak Ünver Koluman , Yücel Doğruel Ahmet Koluman , Batuhan Bakırarar , Nazlı Çil , Esin Avcı , Emine Tural , Fatih Yakar

https://doi.org/10.29058/mjwbs.1058381

Öz

Amaç: Hemostaz, ameliyatın başarısı için hayati bir aşamadır. Düşük maliyetli ve güvenilir bir ajan olan Ankaferd kanama durdurucu (AKD)
pek çok ameliyatta kullanılmasına rağmen henüz kafa içi bölgede kullanıma sunulmamıştır. Bu çalışma, AKD’nun sitotoksik etkilerini ve
memeli beyin parankimasında güvenlik profilini ortaya çıkarmayı amaçlamaktadır.
Gereç ve Yöntemler: 30 Wistar Albino sıçan, 10 sıçandan oluşan üç gruba ayrıldı. Beyin parankim hasarına bağlı kanamalarda grup 1’de
salin, grup 2’de %50 seyreltilmiş AKD ve grup 3’te %100 AKD ile hemostaz sağlandı. Ameliyat öncesi ve sakrifiye edilirken alınan kan
örneklerinde ürotensin, Antitrombin III (AT3) ve fibrinojen çalışıldı. Ayrıca sıçanların sakrifiye edilmesinden sonra histolojik inceleme yapıldı
ve yaralanma skorları değerlendirildi.
Bulgular: Ameliyat öncesi alınan kan örneklerinde grup 2 ve 3’teki fibrinojen düzeyleri grup 1’e göre anlamlı derecede yüksekti. Her üç
grupta da ameliyat öncesi döneme kıyasla sakrifikasyon sırasında ürotensin’de önemli bir artış vardı. (p=0,005) Grup 2’de hafif yaralanma,
grup 3’te hafif yaralanma ve grup 1’de ciddi yaralanma istatistiksel olarak anlamlı derecede yüksekti. (p=0.005) Bu sonuçlar %50 seyreltilmiş
AKD kullanımının güvenli olduğunu göstermektedir.
Sonuç: Memeli beyin parankiminde ilk kez kullanılan AKD, sıçanlarda güvenli olarak değerlendirildi. Hemostatik matriks ajanlarla
karşılaştırıldığında, güvenlik ve etkinliğe ek olarak, düşük maliyeti gelecekte klinik kullanımını artırabilir.

Anahtar Kelimeler

Ankaferd kanama durdurucu, Kraniyal Cerrahi, Hemostaz

Destekleyen Kurum

Pamukkale Üniverstesi Bilimsel Araştırma Projesi Koordinatörlüğü Hızlı Destek Projesi

Proje Numarası

2020HZDP016

Teşekkür

Yok

The Haemostatic Effects of Ankaferd Blood Stopper® on Mammalian Brain Parenchyma: An Experimental Study

Öz

Aim: Haemostasis is a vital stage for the success of the surgery. Although Ankaferd Blood Stopper
(ABS), a low-cost and reliable agent, is used in many surgeries, it is not yet available for use in the
intracranial area. This study aims to reveal ABS’s cytotoxic effects and safety profile in mammalian
brain parenchyma.
Material and Methods: 30 Wistar Albino rats were divided into three groups consisting of 10 rats.
Haemostasis was achieved with saline in group 1, 50% diluted ABS in group 2, and 100% ABS in
group 3 in bleeding caused by damage to the brain parenchyma. Urotensin, Antithrombin III (AT3) and
fibrinogen were studied in blood samples taken before surgery and during sacrification. In addition, the
histologic examination was performed after the sacrification of rats and injury scores were assessed.
Results: Fibrinogen levels in groups 2 and 3 were significantly higher than group 1 in blood samples
taken before surgery. There was a significant increase in urotensin during sacrification compared to
the pre-surgical period in all three groups. (p=0.005) Slight injury in group 2, mild injury in group 3, and
severe injury in group 1 were statistically significantly higher. (p=0.005) These results indicate that the
use of 50% diluted ABS is safe.
Conclusion: ABS, used for the first time in the mammalian brain parenchyma, was evaluated as safe
in rats. Compared to haemostatic matrix agents, in addition to safety and efficacy, its low cost might
increase its clinical use in the future.

Anahtar Kelimeler

Ankaferd Blood Stopper, Cranial surgery, Haemostasis

Proje Numarası

2020HZDP016

Kaynakça

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