Evaluation of Micronucleer and Binucleer Cells Frequencies in Buccal Epithelial Cells of Children Infected with Helicobacter pylori

Öz

Aim: Helicobacter pylori (H. pylori) is a bacterial pathogen that causes gastroduodenal inflammation, ulcer and atrophic gastritis. Micronuclei (MNs) are formations that are not included in the main nucleus that emerge during the mitosis of the cell. In this study, it was aimed to evaluate the relationship between H. pylori and micronuclear and binuclear cells using exfoliative cytology method. Material and Methods: In this study, patients who applied to the Pediatric Gastroenterology outpatient clinic of Zonguldak Bülent Ecevit University Practice and Research Hospital with severe dyspeptic complaints that would affect their quality of life and underwent endoscopic biopsy were included. Biopsy samples were fixed in formalin and sections prepared from paraffin blocks were stained with Hematoxylin & Eosin (H&E). It was evaluated light microscopically for the presence of H. pylori. Only H. pylori positive specimens as a result of biopsy were taken as the study group (n=30), and those with no infectious agents were taken as the control group (n=30). Buccal epithelial cells from all patients were spread on slides, fixed in 95% ethanol and stained according to the Papanicolaou technique. Micronuclear and binuclear cells were counted in 1000 well-stained epithelial cells. Statistical evaluation SPSS 18.0 (SPSS Inc., Chicago, IL, USA) program was used and p<0.05 was considered significant. Results: The children in our study were between the ages of 7 and 15, and the mean age of the study group was 11.87±2.92, and the control group was 11.63±2.73. Micronuclear and binuclear cells were found to be significantly higher in H. pylori positive children compared to the control group (p<0.001). Conclusion: Micronuclear and binuclear cells emphasized that genotoxic damage and irregular cytoplasm division are important in the pathogenesis of H.pylori. Although it is thought that the method used may be a candidate to be an easy-to-apply, low-cost non-invasive diagnostic method in the preliminary diagnosis of H. pylori, this result needs to be confirmed with a larger sample.

Anahtar Kelimeler

• mikronucleus
• binuclear cell
• DNA damage
• PAP technique
• Helicobacter pylori

Helicobacter pylori ile Enfekte Çocukların Yanak Epiteli Döküntü Hücrelerinde Mikronükleer ve Binükleer Hücre Sıklığının Değerlendirilmesi

Öz

Amaç: Helicobacter pylori (H. pylori) gastroduodenal inflamasyon, ülser ve atrofik gastrite yol açan bakteriyel bir patojendir. Mikronüklus (MN)’ lar hücrenin mitoz bölünmesi sırasında ortaya çıkan esas çekirdeğe dahil olmayan oluşumlardır. Bu çalışmada, H. pylori ile mikronükleer ve binükleer hücre arasındaki ilişkinin eksfolyatif sitoloji yöntemi ile değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmada Zonguldak Bülent Ecevit Üniversitesi Uygulama ve Araştırma Hastanesi Çocuk Gastroenteroloji polikliniğine yaşam kalitesini etkileyecek tarzda ciddi dispeptik yakınmalarla başvuran ve endoskopik biyopsi yapılan hastalar yer aldı. Biyopsi örnekleri formalinde fikse edildi ve parafin bloklardan hazırlanan kesitler Hematoksilen & Eozin (H&E) ile boyandı. H. pylori varlığı açısından ışık mikroskobik olarak değerlendirildi. Biyopsi sonucu sadece H. pylori pozitif örnekler çalışma grubu (n=30) hiçbir enfeksiyon etkeni saptanmayanlar ise kontrol grubu (n=30) olarak alındı. Tüm hastalardan alınan yanak epitel hücreleri lamlara yayıldı, % 95’lik etil alkolde fikse edildi ve Papanicolaou boyama yöntemine göre boyandı. Mikronükleer ve binükleer hücreler iyi boyanmış 1000 epitel hücrede sayıldı. İstatistiksel değerlendirme SPSS 18.0 (SPSS Inc., Chicago, IL, USA) programı kullanıldı ve p<0.05 anlamlı olarak kabul edildi. Bulgular: Çalışmamızda yer alan çocuklar 7 ile 15 yaşları arasında olup çalışma grubunun yaş ortalaması 11,87± 2,92, kontrol grubunun ise 11,63± 2.73’tür. H. pylori pozitif çocuklarda mikronükleer ve binükleer hücreler kontrol grubuna göre anlamlı derecede yüksek bulundu (p<0.001). Sonuç: Mikronükleer ve binükleer hücreler, H.pylori patogenezinde genotoksik hasarın ve düzensiz sitoplazma bölünmesinin önemli olduğunu vurgulamıştır. Kullanılan yöntemin H. pylori ön tanısında kolay uygulanabilen, az maliyetli invaziv olmayan tanı yöntemi olma yönünde aday olabileceği düşünülmekle birlikte bu sonucun daha geniş örneklemle teyit edilmesine ihtiyaç bulunmaktadır

Anahtar Kelimeler

• Helicobacter pylori
• mikronükleus
• binükleer hücre
• DNA hasarı
• PAP boyama

Teşekkür

Füruzan Köktürk

Kaynakça

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