IL8/CXCL8 Gen Polimorfizminin COVID-19 Şiddeti ile İlişkisi

Öz

Amaç: COVID-19, ilk olarak yüksek ateş ve nefes darlığı ile tanımlanan, damlacık ve temas yoluyla bulaştığı bilinen, viral bir solunum yolu hastalığıdır. Hastalığın klinik seyri kişiden kişiye farklılık göstermektedir. COVID-19’un klinik seyrindeki farklılıklar komorbidite ve yaş gibi etkenlere bağlı olabilmekle birlikte, inflamatuar yanıttaki kişisel genetik farklılıklara da dayanabilir. Bu çalışmada COVID-19 hastalığının klinik şiddeti ile IL8/CXCL8 rs4073 gen polimorfizmi arasındaki ilişkiyi araştırmayı amaçladık. Gereç ve Yöntemler: Klinik şiddetine göre hafif, orta ve şiddetli olmak üzere üç gruba ayrılmış olan 150 COVID-19 hastasında, IL8/CXCL8 rs4073 (-251A/T) gen polimorfizmi polimeraz zincir reaksiyonu tabanlı restriksiyon parçası uzunluk polimorfizmi (PCR-RFLP) yöntemi ile belirlendi. Bulgular: Sonuçlarımız IL8/CXCL8 rs4073 gen polimorfizmi ile hastalık şiddeti arasında genotip ve alel sıklığı dağılımı açısından anlamlı bir fark olmadığını göstermiştir. Ancak klinik bulgular açısından değerlendirildiğinde T allelinin, pnömoni bulgusu olan COVID-19 vakalarında daha sık görülmesi T allelinin COVID-19’da pnömoni riskini 1,7 kat artırdığı belirlenmiştir. Sonuç: Bu çalışmadan elde edilen sonuçlar IL8/CXCL8 rs4073 gen polimorfizminin erken tedavi ve takip sayesinde hastalarda pnömoninin ilerlemesini engellemede kullanılabilecek bir belirteç adayı olabileceğini göstermiştir. Bunun için daha fazla sayıda hasta içeren büyük popülasyonlarda yapılmış yeni çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• COVID-19
• SarsCOV-2
• IL8
• CXCL8
• gen polimorfizmi
• rs4073

The Relationship Between IL8/CXCL8 Gene Polymorphism and the Severity of COVID-19

Öz

Aim: COVID-19 is a viral respiratory disease primarily characterized by high fever and shortness of breath, known to be transmitted through droplets and contact. The clinical course of the disease varies from person to person. Differences in the clinical course of COVID-19 may be attributed to factors such as comorbidities, age, and could also be based on individual genetic variations in the inflammatory response. In this study, we aimed to investigate the relationship between the severity of clinical manifestations of COVID-19 and the IL8/CXCL8 rs4073 gene polymorphism. Material and Methods: The IL8/CXCL8 rs4073 (-251A/T) gene polymorphism was determined using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 150 COVID-19 patients who were classified into three groups based on the severity of their clinical manifestations: mild, moderate, and severe. Results: Our results demonstrated no significant difference in genotype and allele frequency distribution between the IL8/CXCL8 rs4073 gene polymorphism and disease severity. However, when evaluated in terms of clinical findings, the T allele was found to be more common in COVID-19 cases with pneumonia symptoms, and it was determined that the T allele increased the risk of pneumonia in COVID-19 by 1.7 times. Conclusion: The findings from this study suggest that the IL8/CXCL8 rs4073 gene polymorphism could serve as a potential marker that, through early treatment and monitoring, could help prevent the progression of pneumonia in patients. Further studies with larger populations, involving a greater number of patients, are needed to validate these findings.

Anahtar Kelimeler

• COVID-19
• SarsCOV-2
• IL8
• CXCL8
• gene polymorphism
• rs4073

Kaynakça

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