Gizemi Çözmek: Yüksek Dansiteli Lipoprotein 'İyi' Kolesterol Nasıl 'Kötü' Oluyor?

Öz

Arka Plan: Son yıllarda, araştırmalar aterosklerozun ilerlemesinde, özellikle yüksek yoğunluklu lipoprotein (HDL) ile etkileşimi açısından Lektin-benzeri oksitlenmiş düşük yoğunluklu lipoprotein reseptör-1'in (LOX-1) önemini vurgulamıştır. Çalışmamız, Koroner Arter Hastalığı (CAD) olan hastalarda disfonksiyonel Yüksek Yoğunluklu Lipoprotein'in (HDL) Lektin-benzeri oksitlenmiş düşük yoğunluklu lipoprotein reseptör-1'e (LOX-1) bağlanma rolünü ve ateroskleroz ilerlemesi üzerindeki etkilerini araştırmayı amaçladı. Yöntemler: Kasım 2019 ile Nisan 2020 tarihleri arasında iç meme arteri (IMA), radial arter (RA) ve safen ven (SV) greftleri kullanılarak çok damarlı CABG geçiren yirmi bir hasta dahil edildi. Endotel hücrelerini tanımlamak için CD31 kullanılarak, tüm kültürlü hücrelerde LOX-1 ifadesi değerlendirildi. Bulgular: Çalışmamız, 17'si (%80.9) erkek ve 4'ü (%19.04) kadın olmak üzere 21 hastadan oluşmaktadır. Tüm greft türlerinden alınan örneklerde HDL ve LOX-1+ ifadesi arasında anlamlı bir pozitif korelasyon gözlemledik (SV, r=0.60; RA, r=0.48; IMA, r=0.53). IMA için LOX-1+ ifadesindeki varyasyonun yaklaşık %28'i (F=7.03; p<0.05), SV için %36'sı (F=10.10; p<0.05) ve RA için %24'ü (F=5.64; p<0.05) HDL'ye atfedildi. Sonuçlar: Bulgularımız, yüksek LOX-1 reseptör ifadesi olan durumlarda HDL'in LOX-1'e bağlanarak işlevselliğini kaybettiğini öne sürmektedir. HDL ve LOX-1 seviyelerinin izlenmesi, hastaların tedaviye yanıt verme tahminini artırabilir ve uygun müdahaleleri belirlemeye yardımcı olabilir.

Anahtar Kelimeler

• Ateroskleroz
• koroner arter bypas greftleri
• kardiyovasküler hastalık
• lipoprotein

Proje Numarası

TTU-2019-18259

Unraveling the Mystery: How High Density Lipoprotein ‘Good’ Cholesterol Goes ‘Bad’?

Öz

Background: In recent years, research has highlighted the importance of Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in the progression of atherosclerosis, particularly its interaction with high-density lipoprotein (HDL). Our study aimed to explore the role of dysfunctional High-Density Lipoprotein (HDL) in binding to Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) among patients with Coronary Artery Disease (CAD) and its implications for the advancement of atherosclerosis. Methods: We enrolled twenty-one patients who underwent multivessel CABG using internal mammary artery (IMA), radial artery (RA), and saphenous vein (SV) grafts between November 2019 and April 2020. Using CD31 to identify endothelial cells, we evaluated LOX-1 expression in all cultured cells. Results: Our study comprised 21 patients, including 17 (80.9%) males and 4 (19.04%) females. We observed a significant positive correlation between HDL and LOX-1+ expression in specimens extracted from all types of grafts (SV, r=0.60; RA, r=0.48; IMA, r=0.53). Approximately 28% of the variation in LOX-1+ expression for IMA (F=7.03; p<0.05), 36% for SV (F=10.10; p<0.05), and 24% for RA (F=5.64; p<0.05) was attributed to HDL. Conclusions: Our findings suggest that in cases with heightened LOX-1 receptor expression, HDL binds to LOX-1, leading to a loss of functionality. Monitoring HDL and LOX-1 levels could enhance the prediction of patient responsiveness to treatment and inform appropriate interventions

Anahtar Kelimeler

• Atherosclerosis
• coronary artery bypass grafts
• cardiovascular disease
• lipoprotei

Destekleyen Kurum

Hacettepe University Scientific Research Projects Coordination Unit

Proje Numarası

TTU-2019-18259

Kaynakça

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