Effect of Thyroid Hormone Pre-conditioning on Intestinal and Lung Damage in The Intestinal Ischemia/Reperfusion Model

Yıl 2017, Cilt: 1 Sayı: 3, 104 - 111, 29.12.2017

İnci Turan Hale Sayan Özaçmak , Figen Barut Veysel Haktan Özaçmak Bülent Uçan

Öz

Pre-conditioning of 1-3,3 ', 5-triiodothronine (T3) or 1-tyroxine (T4)
in various experimental studies has been shown to be protective
against hepatic, brain, cardiac and renal ischemia reperfusion (I/R)
damage. Intestinal I/R injury, a critical condition usually caused by
many clinical scenarios such as shock, acute mesenteric ischemia,
sepsis, mesenteric thrombosis, or bowel transplantation. The purpose
of this study is to examine the possible protective effect of
preconditioning with T3 against damage to the small intestine and
lung tissue caused by intestinal I / R.
Male Wistar Albino rats were subjected to mesenteric ischemia (30
min) followed by reperfusion (3h). Four groups were designed, each
of contain 8 animals: sham control; I/R control; I/R +50µg/kg T3
administration; and I/R+100µg/kg T3 administration. Intestinal and
lung tissue damage was examined histopathologically.
Our results indicate that 100 µg/kg T3 preconditioning attenuates the
I/R-induced histopathological changes in intestinal and lung tissues.
Histopathological damage assessment in both lung and intestinal
tissue was found to be high in the I/R group. In the 100µg T3 treated
group, intestinal mucosa and lung injury were found to be lower than
I / R and 50µg T3 treated groups.
In conclusion, it was observed that T3-induced preconditioning
before intestinal I/R injury was histopathologically protective effect
on small intestine and lung tissue caused by intesinal ischemia.

Anahtar Kelimeler

Thyroid Hormone, Intesinal Ischemia, Lung Injury, Reperfusion

İntestinal İskemi/Reperfüzyon Modelinde Tiroid Hormon Ön Koşullanmasının İnce Bağırsak ve Akciğer Hasarı Üzerine Etkisi

Öz

Çeşitli deneysel çalışmalarda l-3,3′,5- triiodotronin (T3) veya ltiroksin
(T4) önkoşullanmasının karaciğer, beyin, kalp ve böbrek
iskemi reperfüzyon (İ/R) hasarına karşı koruyucu etkili olduğu
gösterilmiştir. İntestinal I/R hasarı, genellikle şok, akut mezenterik
iskemi, sepsis, mezenterik tromboz veya bağırsak nakli gibi birçok
klinik durumun neden olduğu kritik bir tablodur. Bu çalışmanın
amacı T3 ile oluşturulan önkoşullanmanın intestinal İ/R ile oluşan
ince bağırsak ve akciğer dokusundaki hasara karşı olası koruyucu
etkisini incelemektir.
Erkek Wistar Albino cinsi sıçanlara 30 dakika mezenterik iskemi ve
sonrasında 3 saat reperfüzyon uygulandı. Her grupta 8 hayvan olmak
üzere 4 gruba ayrıldılar: sham kontrol, İ/R kontrol, İ/R +50µg/kg T3
uygulanan ve İ/R+100µg/kg T3 uygulanan grup. İntestinal ve
akciğer dokusundaki hasar histopatolojik olarak incelenmiştir.
Sonuçlarımız ince bağırsak ve akciğer dokusunda 100 µg/kg
dozunda T3 önkoşullanmasının İ/R ile oluşan patolojik değişikleri
azalttığını göstermektedir. İ/R grubunda hem akciğer hem de
intestinal dokuda histopatolojik hasar değerlendirmesinin yüksek
seviyesinde olduğu bulunmuştur. 100 µg İ/R +T3 uygulanan grupta
intestinal mukoza ve akciğer hasarının İ/R ve İ/R +50µg T3
uygulanan gruba göre düşük olduğu saptanmıştır.
Sonuç olarak, intestinal I/R hasarından önce T3 ile oluşturulan
önkoşullanmanın bağırsak iskemisinin neden olduğu ince bağırsak
ve akciğer dokusu üzerinde histopatolojik olarak koruyucu etkili
olduğu gözlemlendi.

Anahtar Kelimeler

Tiroid Hormon, İntestinal İskemi, Akciğer Hasarı, Reperfüzyon

Kaynakça

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