Yemlere ilave edilen üzüm çekirdeği ekstraktının Gökkuşağı alabalığının bazı biyokimyasal parametrelerine etkisi

Yıl 2022, Cilt: 27 Sayı: 3, 623 - 628, 01.12.2022

Mikail Özcan , Ünal İspir , Ayşe Şahin

https://doi.org/10.37908/mkutbd.1057918

https://izlik.org/JA25BZ64JU

Öz

Amaç: Bu çalışmada, üzüm çekirdeği ekstraktının (GSE), ortalama ağırlığı 42.5±1.44 g olan gökkuşağı alabalığının (Oncorhynchus mykiss) bazı biyokimyasal parametreleri üzerine etkisi araştırılmıştır.

Yöntem ve Bulgular: Balıklar, 42 gün boyunca %0 (i), %0.5 (ii), %1(iii) ve %1.5 (iv) GSE içeren yemlerle beslenmiştir. Deney bitiminde balıklardan dalak, karaciğer, böbrek ve kas dokuları alınarak toplam protein, toplam lipit ve toplam karbonhidrat miktarları belirlenmiştir.

Genel Yorum: Tüm deney gruplarının toplam protein seviyelerinin, diyet GSE takviyesi ile önemli ölçüde arttığı belirlenmiştir (p<0.05). Gökkuşağı alabalığının toplam karbonhidrat ve toplam lipid düzeylerinin, tüm dokularda diyetsel GSE düzeyinden önemli ölçüde etkilenmediği tespit edilmiştir.

Çalışmanın Önemi ve Etkisi: Sonuç olarak, diyet GSE takviyesi ile gökkuşağı alabalığının bazı biyokimyasal parametrelerinde bir gelişme olduğu gözlemlenmiştir.

Anahtar Kelimeler

Üzüm çekirdeği ekstraktı , Gökkuşağı alabalığı , Oncorhynchus mykiss , total Protein , total Lipid , total Karbonhidrat.

Effects of dietary grape seed extract supplementation on some biochemical parameters of Rainbow trout

https://izlik.org/JA25BZ64JU

Öz

Aims: The goal of this research was to investigate the impact of grape seed extract on some biochemical parameters of rainbow trout (Oncorhynchus mykiss) with an average weight of 42.5±1.44g.

Methods and Results: Fishes were fed with diets containing 0%, 0.5%, 1% and 1.5% of grape seed extract (GSE) during 42 days. At the end of the experiment, kidney, spleen, muscle and liver tissues were taken from fish and total protein, total lipid and total carbohydrate levels were determined.

Conclusions: The total protein levels of 0%, 0.5%, 1% and 1.5% group were increased importantly by dietary GSE supplementation (p<0.05). On the other hand, the total carbohydrate and total lipid levels were not significantly affected by the dietary level of GSE in all tissues.

Significance and Impact of the Study: In conclusion, we demonstrated an improvement of total protein, total lipid and total carbohydrate parameters of Oncorhynchus mykiss by dietary GSE supplementation. 

Anahtar Kelimeler

Grape seed extract , Rainbow trout , Oncorhynchus mykiss , Total Protein , Total Lipid , Total Carbohydrate

Teşekkür

This study was presented as a poster presentation at the FABA, 03- 05/11/2016 in Antalya, Turkey.

Kaynakça

• AOAC (1990) Official methods of analysis of the AOAC, 15th ed. Methods 932.06, 925.09, 985.29, 923.03. Association of official analytical chemists. Arlington, VA, USA.
• Boghdady NA (2013) Antioxidant and antiapoptotic effects of proanthocyanidin and ginkgo biloba extract against doxorubicininduced cardiac injury in rats. Cell Biochem. Funct. 31: 344-351.
• Deis RC (2006) The complexity of shelf life stability, 1 st. USA: Virgo pub.
• Dulundu E, Ozel Y, Topaloglu U, Toklu H, Ercan F, Gedik N, Sener G (2007) Grape seed extract reduces oxidative stress and fibrosis in experimental biliary obstruction. J. Gastroen. Hepatol. 22: 885-892.
• El-Ashmawy IM, Gad SB, Salama OM (2010) Grape seed extract prevents azathioprine toxicity in rats. Phytother Res. 24:1710-1715.
• Espe M, Sveier H, Hogoy I, Lied E (1999) “Nutrient absorption and growth of Atlantic salmon (Salmo salar L.) fed fish protein concentrate,” Aquaculture 174, 1-2: 119–137.
• Girgin Basusta A (2005) Balık biyolojisi araştırma yöntemleri, Nobel Yayın, Ankara, 772 498.
• Hassan HA, Al-Rawi MM (2013) Grape seeds proanthocyanidin extract as a hepaticreno- protective agent against gibberellic acid induced oxidative stress and cellular alterations. Cytotechnology 65: 567-576.
• Hoşsu B, Korkut AY, Fırat A (2001) Balık Besleme ve Yem Teknolojisi I. Ege Üniversitesi Su Ürünleri Fakültesi Yayınları No:50. Basımevi, Bornova, İzmir, 276s.
• Jauncey K (1982) The effects of varying dietary protein level on the growth, food conversion, protein utilization and body composition of juvenile tilapias (Sarotherodon mossambicus). Aquaculture 27 (1), 43–54.
• Jayaprakasha GK, Selvi T, Sakariah KK (2003) Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Res. Int. 36 (2), 117–122.
• Kao TT, Tu HC, Chang WN, Chen BH, Shi YY, Chang TC, Fu TF (2010) Grape seed extract inhibits the growth and pathogenicity of Staphylococcus aureus by interfering with dihydrofolate reductase activity and folate-mediated one-carbon metabolism. Int. J. Food Microbiol. 141:17-27.
• Kesbiç OS, Yigit M (2019) Structural and chemical changes of grape seed extract after thermal processing and its use in rainbow trout (Oncorhynchus mykiss) diets as an organic feed supplement. Aquaculture 503: 275-281.
• Mehrabi Z, Firouzbakhsh F, Jafarpour A (2012) Effects of dietary supplementation of synbiotic on growth performance, serum biochemical parameters and carcass composition in rainbow trout (Oncorhynchus mykiss) fingerlings. J. Anim. Physiol. Anim. Nutr. 96 (3): 474–481.
• Min S, Rumsey TR, Krochta J (2006) Lysozyme diffusion in smoked salmon coated with whey protein films in corporation lysozyme, CDRF.
• Raa J, Rorstad G, Engstad RE, Robertson B (1992) “The use of immunostimulants to increase resistance of aquatic organism to microbial infections,” in Disease in Asian Aquaculture. Proceedings of the First Symposium on Disease in Asian Aquaculture, M. Shariff, R. P. Subasingh, and J. R.Arthur, Eds., vol. 1, pp. 39–50, Asian Fisheries Society, Manila, Philippines,
• Tacon AG, Metian M (2015) Feed matters: satisfying the feed demand of aquaculture. Reviews in Fisheries Science & Aquaculture, 23(1), 1-10.
• Tacon, A. G. (1993). Feed ingredients for warmwater fish, fish meal and other processed feedstuffs. FAO Fisheries Circular (FAO). no. 856.
• Wilska-Jeszka J (1996) Proanthocyanidins: content in fruits and influence on health. Food Chem. 57(1):57-59.
• Yousef MI, Saad A.A, El-Shennawy LK (2009) Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food Chem. Toxicol. 47:1176-1183.
• Zhai SW, Lu JJ, Chen XH (2014) Effects of dietary grape seed proanthocyanidins on growth performance, some serum biochemical parameters and body composition of tilapia (Oreochromis niloticus) fingerlings. Italian Journal of Animal Science, 13(3), 3357.