Sığır embriyolarında 2G/3G cep telefonu sinyallerinin in vitro fertilizasyon, embriyo gelişimi ve cinsiyet dağılımı üzerine etkileri

Öz

Bin yıllık insan evrimi boyunca eşit olan erkek/dişi oranı, son yıllarda bir şekilde bu dengeden uzaklaşmaktadır. Yapılan bu çalışmanın amacı, cep telefonlarından yayılan elektromanyetik radyasyonun sığır embriyolarında in vitro fertilizasyon, embriyo gelişimi ve cinsiyet farklılaşması üzerindeki etkilerinin araştırılması olmuştur. Kesimhaneden alınan ovaryumlardan elde edilen MII oositler deneme materyali olarak kullanılmıştır. İnkübatör içerisine yerleştirilen bir cep telefonunun belirli aralıklarla çaldırılması ile gametler elektromanyetik alana maruz bırakılmıştır. Embriyo gelişim aşamaları, bölünme ve 7-8’inci günlerdeki blastosist gelişim aşamaları bakımından takip edilmiştir. İn vitro koşullarda üretilen embriyolarda cinsiyet tayini polimeraz zincir reaksiyon (PCR) ile belirlenmiştir. Sonuç olarak radyofrekans elektromanyetik alanların embriyo gelişimi üzerine olumsuz bir etkisinin olmadığı, ancak radyofrekans elektromanyetik alanların blastomer sayısını, embriyo gelişim aşamasını ve embriyo kalitesini azaltabileceği belirlenmiştir. Ayrıca elektromanyetik alanlara maruz kalmanın erkek embriyoların yaşama şansını büyük ölçüde azalttığı tespit edilmiştir.

Anahtar Kelimeler

• Elektromanyetik alan
• Embriyo
• Cinsiyet
• IVF
• Sığır

Effects of exposure to 2G/3G cell phone radiation on in vitro fertilization, subsequent development and sex distribution of bovine embryos

Abstract

During the thousand years of human evolution, the male to female ratio was practically equal, but it has recently changed in some way. The aim of this study was to investigate the effects of electromagnetic radiation from mobile phones on in vitro fertilization, embryo growth, and sex differentiation in cattle embryos. MII oocytes obtained from ovaries taken from slaughterhouse were used as research material. Gametes were exposed to electromagnetic fields by having a mobile phone inside the incubator that would periodically ring. On days 7 and 8, blastocyst development stages and embryo cleavage rates were evaluated. Additionally, the rates of cleavage for different time intervals after in vitro fertilization were noted. The sex determination of the embryos produced in vitro was determined by using polymerase chain reaction (PCR). As a result, it was found that exposure to radiofrequency electromagnetic fields could mainly reduce blastomere count, embryo diameter, and embryo quality rather than a having major adverse effect on the development of cattle embryos. Additionally, it was shown that exposure to electromagnetic fields appears to drastically reduce the chances of male survival.

Keywords

• Electromagnetic field
• Embryo
• Sex ratio
• IVF
• Bovine

Kaynakça

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