Hatay sarısı ırkı ipekböceği (Bombyx mori L.) ile ilişkili bakteri çeşitliliği: İzolasyon, teşhis ve karakterizasyon

Öz

Nesli tükenme tehlikesiyle karşı karşıya olan Hatay sarı ırkı ipekböceği (Bombyx mori L.), Türkiye'nin en önemli yerel kültür miraslarından biridir. İpekböceğinin bakteriyel patojenleri son derece yıkıcı olup, çoğunlukla akut hastalıklara neden olurlar. Bu çalışmanın amacı, Hatay sarı ırkının enfekteli ve ölü larvalarında bakteri çeşitliliğini ve potansiyel patojenik bakteri türlerini belirlemektir. Hatay sarı ırkından izole edilen toplam 16 bakteri izolatı morfolojik, biyokimyasal ve moleküler özelliklerine göre tanılanmıştır. Hatay sarı ırkının enfekteli ve ölü larvalarından izole edilen bakteri izolatları Staphylococcus sp. (BM-1), Staphylococcus xylosus (BM-5), Staphylococcus succinus (BM-7), Bacillus thuringiensis (BM-8), Bacillus subtilis (BM-9), Bacillus sp. (BM-10), Staphylococcus saprophyticus (BM-16, BM-19), Klebsiella sp. (BM-17), Staphylococcus arlettae (BM-18), Pseudomonas aeruginosa (BM-20), Enterococcus mundtii (BM-21), Pantoea agglomerans (BM-22), Kluyvera intermedia (BM-23), Serratia sp. (BM-24), Mammaliicoccus sciuri (BM-25) olarak belirlenmiştir. Bakteri yoğunluğu ve tür sayısının fazla olması Hatay sarı ırkının bakteriyel hastalıklara karşı son derece duyarlı olduğunu göstermektedir. İnsektisidal etkinlik çalışmaları, Bacillus ve Staphylococcus cinslerine ait türlerin hibrit ipekböceği kültürü ve Hatay sarı ırkında önemli patojenleri olduğunu ortaya koymuştur.

Anahtar Kelimeler

• İpekböceği Hatay sarı suşu
• Bombyx mori L.
• Yerli ipekböceği cinsi
• Patojen bakteri

Bacterial diversity associated with the Hatay yellow strain silkworm (Bombyx mori L.): Isolation, identification and characterization

Abstract

The Hatay yellow strain silkworm (Bombyx mori L.), which is in danger of extinction, is one of the most important local cultural heritages of Türkiye. Bacterial pathogens of silkworm are highly destructive and cause mostly acute diseases. The aim of this study was to determine the bacterial diversity and potential pathogenic bacterial species in infected and dead larvae of Hatay yellow race. A total of 16 bacterial isolates from Hatay yellow race were identified according to their morphological, biochemical and molecular characteristics. The bacterial isolates isolated from infected and dead larvae of Hatay yellow race were Staphylococcus sp. (BM-1), Staphylococcus xylosus (BM-5), Staphylococcus succinus (BM-7), Bacillus thuringiensis (BM-8), Bacillus subtilis (BM-9), Bacillus sp. (BM-10), Staphylococcus saprophyticus (BM-16, BM-19), Klebsiella sp. (BM-17), Staphylococcus arlettae (BM-18), Pseudomonas aeruginosa (BM-20), Enterococcus mundtii (BM-21), Pantoea agglomerans (BM-22), Kluyvera intermedia (BM-23), Serratia sp. (BM-24), Mammaliicoccus sciuri (BM-25). The high bacterial density and number of species indicate that Hatay yellow race is highly susceptible to bacterial diseases. Insecticidal activity studies revealed that species belonging to Bacillus and Staphylococcus genera are important pathogens of hybrid silkworm culture and Hatay yellow race.

Keywords

• Silkworm Hatay yellow strain
• Bombyx mori L.
• Domestic silkworm breed
• Pathogen bacteria

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