Determination of polymorphisms in the HSP90AA1 gene region in some Turkish sheep populations by AS-PCR

Öz

Heat shock proteins (HSPs) are molecular chaperones protecting living cells from the negative effects of extreme ambient temperatures. In this study, genetic polymorphisms of the HSP90AA1 gene were monitored via Allele-Specific Polymerase Chain Reaction (AS-PCR) in three native Turkish sheep populations namely İvesi (IVS), Güney Karaman (GKR), and Karakaş (KRK). The HSP90AA1 was polymorphic in all populations yielding two alleles (C and G) and three genotypes (CC, CG, and GG). The G allele frequency was higher than the C allele frequency in all populations. The lowest (0.100) and highest (0.246) CC frequency was observed in KRK and IVS, respectively, while the GG genotype frequency varied between 0.250 (GKR) and 0.600 (KRK). Significant deviation (p<0.001) from Hardy-Weinberg Equilibrium (HWE) was detected in the IVS breed in terms of the HSP90AA1 gene. The genetic distance-based phylogenetic tree indicated that GKR was genetically different from IVS and KRK populations in terms of the HSP90AA1 polymorphism. These variations regarding the HSP90AA1 gene should be conserved, since the negative effects of global warming and climate change are expected to be more hazardous in the future. Besides, these genetic variations may be utilized by the farmers to design comprehensive selection strategies against heat stress in native Turkish sheep populations.

Anahtar Kelimeler

• AS-PCR
• HSP
• heat stress
• polymorphism

Bazı Türkiye yerli koyun populasyonlarında HSP90AA1 gen bölgesindeki polimorfizlerin AS-PZR ile belirlenmesi

Öz

Isı şok proteinleri (HSPs) canlı hücreleri aşırı ortam sıcaklıklarının olumsuz etkilerinden koruyan moleküler şaperonlardır. Bu çalışmada, İvesi (IVS), Güney Karaman (GKR) ve Karakaş (KRK) olarak bilinen üç farklı Türkiye yerli koyun populasyonunda HSP90AA1 genindeki genetik polimorfizmler Allel-Spesifik Polimeraz Zincir Reaksiyonu (AS-PZR) yöntemiyle incelenmiştir. İki allel (C ve G) ve üç genotipin (CC, CG ve GG) belirlendiği çalışmada, HSP90AA1 geni bütün populasyonlarda polimorfizm göstermiştir. Bütün populasyonlarda G allel frekansı C allel frekansından yüksek bulunmuştur. En düşük (0.100) ve en yüksek (0.246) CC frekansı sırasıyla KRK ve IVS populasyonlarında belirlenirken, GG genotip frekansı 0.250 (GKR) ile 0.600 (KRK) aralığında değişmiştir. IVS ırkında HSP90AA1 geni bakımından Hardy-Weinberg dengesinden (HWE) olan sapma önemli (p<0.001) bulunmuştur. Genetik mesafe temelli filogenetik ağaç, HSP90AA1 polimorfizmi bakımından GKR populasyonunun IVS ve KRK populasyonlarından genetik olarak farklı olduğunu ortaya koymuştur. Gelecekte küresel ısınma ve iklim değişikliğinin olumsuz etkileri daha da tehlikeli olacağından HSP90AA1 genindeki bu varyasyonların korunması gerekmektedir. Ayrıca, bu genetik varyasyonlar çiftçilerin Türkiye yerli koyun populasyonlarında ısı stresine karşı yapılacakları kapsamlı seleksiyon çalışmalarında kullanılabilecektir.

Anahtar Kelimeler

• AS-PCR
• Isı stresi
• HSP
• Polimorfizm

Kaynakça

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