Insecticidal efficiency of green-synthesized zinc oxide nanoparticles on bean weevil, Acanthoscelides obtectus Say. (Coleoptera: Chrysomelidae)

Öz

The use of zinc oxide nanoparticles (ZnO-NPs) as a bio-insecticide has gained increasing attention due to their eco-friendly properties and proven efficacy in controlling pest populations. This study comprehensively assessed the insecticidal activity of ZnO-NPs against the bean weevil, Acanthoscelides obtectus Say. (Coleoptera: Chrysomelidae) adults, by analyzing mortality rates in a dose-dependent manner under controlled laboratory conditions. ZnO-NPs were synthesized and characterized using Scanning Electron Microscopy (SEM), which confirmed their spherical shape and nanoscale dimensions (~100 nm). Adult mortality was monitored over a 10-day period at doses of 100, 250, 500, 750 and 1000 mg kg⁻¹. The two-way ANOVA results revealed that both dose and exposure duration had significant effects on insect mortality rates. Furthermore, one-way ANOVA showed a significant dose-dependent increase in mortality, with the highest dose (1000 mg kg⁻¹) achieving a corrected mortality rate of 93.3%. These findings underscore the potential of ZnO-NPs as a sustainable alternative to conventional chemical insecticides, particularly for integrated pest management strategies in stored products. Future studies should focus on optimizing control practices in storage facilities and evaluating the long-term effects on non-target organisms and explore various doses and application methods to ensure safe and effective use.

Anahtar Kelimeler

• Insecticidal effect
• ZnO nanoparticles
• Acanthoscelides obtectus
• Bio-insecticide
• Pest management

Yeşil sentez ile üretilen çinko oksit nanopartiküllerinin fasulye tohum böceği, Acanthoscelides obtectus Say. (Coleoptera: Chrysomelidae) üzerindeki insektisidal etkinliği

Öz

Çinko oksit nanopartiküllerinin (ZnO-NP) biyo-insektisit olarak kullanımı, çevre dostu özellikleri ve zararlı popülasyonlarının mücadelesinde giderek daha fazla ilgi görmektedir. Bu çalışmada, ZnO nanopartiküllerinin fasulye tohum böceği, Acanthoscelides obtectus Say. (Coleoptera: Chrysomelidae) erginleri üzerindeki insektisidal aktivitesi, kontrollü laboratuvar koşulları altında farklı dozlarda ölüm oranlarının analiz edilmesi yoluyla kapsamlı bir şekilde değerlendirilmiştir. ZnO nanopartikülleri, Taramalı Elektron Mikroskobu (SEM) kullanılarak sentezlenmiş ve karakterize edilmiş, küresel şekilleri ve nanoskaladaki boyutları (~100 nm) doğrulanmıştır. Ergin ölüm oranı, 100, 250, 500, 750 ve 1000 mg kg⁻¹ dozlarında 10 günlük bir süre boyunca izlenmiştir. İki yönlü ANOVA sonuçları, hem dozun hem de maruz kalma süresinin böceklerin ölüm oranı üzerinde anlamlı etkileri olduğunu göstermiştir. Ayrıca, Tek Yönlü ANOVA ile yapılan istatistiksel analiz, ölüm oranlarında doza bağlı olarak anlamlı bir artış olduğunu göstermiş ve en yüksek dozda (1000 mg kg⁻¹), ölüm oranları %93.3’e ulaşmıştır. Bu bulgular, ZnO nanopartiküllerinin özellikle depolanmış ürünlerde entegre zararlı yönetimi stratejileri için insektisitlere sürdürülebilir bir alternatif olarak önemli potansiyelini vurgulamaktadır. Gelecekteki çalışmalar, depolardaki mücadele uygulamalarını optimize etmeye ve hedef dışı organizmalar üzerindeki uzun vadeli etkileri değerlendirmeye ve farklı doz ve uygulama yöntemlerinin güvenli ve etkili kullanımına yönelik araştırmaların yapılmasına odaklanmalıdır.

Anahtar Kelimeler

• ZnO nanopartikülleri
• Acanthoscelides obtectus
• Biyo-insektisit
• Zararlı yönetimi
• İnsektisidial etki

Kaynakça

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