The effects of silicon pre – treatment on physiological and biochemical responses of maize under water deficit

Öz

The present study was conducted to investigate the effects of silicon (Si) pre – treatment under different irrigation regimes on growth, gas – exchange properties and physiological features of maize in the “Selcuk University, Agriculture Faculty, Field Crops Department, Research and Practicing Station of Prof. Dr. Abdulkadir AKÇIN, Konya, Türkiye” during 2019 and 2020 growing seasons. Irrigations (50%, 75%, 100% of total evaporation from Class A Evaporation Pan), genotypes (purple and white coloured corns) and treatments (control, 1.5mM Si) were the factors of the trial. This study found out that water restriction (I1 and I2) adversely affected maize growth attributes (plant height, leaf number, leaf area) and photosynthetic efficiency (A). However, Si priming significantly improved drought tolerance, largely mitigating these negative effects. Plant height and proline concentration of the silicon – primed purple corn was higher than control under I1 during both years of the experiment. Silicon priming increased photosynthesis rate (46.20%), stomatal conductance (35.47%) and total anthocyanin content (12.80%) of the purple corn during 2019 whilst it increased total anthocyanin content (10.10%) of the white corn during 2020. Besides the healing effects of Si pre – soaking under I1, it had positive impacts on many features under I2 and I3. According to the results of the experiment it may be concluded that Si pre – treatment may be an alternative strategy to increase performance and resilience of maize under water restricted conditions.

Anahtar Kelimeler

• anthocyanins
• maize
• physiology
• pre – treatment
• proline

Etik Beyan

This article does not require ethical approval as there are no experiments with human or animal subjects.

Silikon ön – uygulamasının mısırın kısıtlı su koşullarındaki fizyolojik ve biyokimyasal tepkilerine etkisi

Öz

Bu çalışma 2019 – 2020 yetiştirme sezonlarında mısırda silikon (Si) ön uygulamasının farklı sulama rejimlerinde büyüme, gaz değişim özellikleri ve fizyolojik özellikler üzerindeki etkilerini belirlemek amacıyla “Selçuk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Prof. Dr. Abdulkadir AKÇIN Araştırma ve Uygulama İstasyonu, Konya, Türkiye” de yürütülmüştür. Denemede faktör olarak sulamalar (Class A Evaporasyon kabından gerçekleşen buharlaşmanın %50, %75 ve %100’ ü), genotipler (mor ve beyaz renkli mısırlar) ve uygulamalar (kontrol ve 1.5 mM Si) yer almıştır. Bu çalışmada su kısıtlamasının (I1 ve I2) mısırın büyüme parametreleri (bitki boyu, yaprak sayısı ve yaprak alanı) ve fotosentetik verimini (A) önemli ölçüde etkilediği görülmüştür. Ancak Si priming kuraklığa toleransı bu olumsuz etkileri azaltarak önemli ölçüde arttırmıştır. Denemenin iki yılında da I1’ de Si priming yapılmış mor mısırın bitki boyu ve prolin konsantrasyonu kontrolden yüksek olmuştur. Silikon priming, 2019 da mor mısırın fotosentez oranı (%46.20), stoma iletkenliği (%35.47) ve total antosiyanin içeriğini (%12.80), 2020 de ise beyaz mısırın total antosiyanin içeriğini (%10.10) arttırmıştır. Silikon ön uygulamasının I1’ deki olumlu etkilerinin yanında, I2 ve I3 te de çok sayıda özellik üzerinde olumlu etkileri olmuştur. Denemeden elde edilen bulgulara göre Si ön uygulamasının mısırın kısıtlı sulama koşullarında performans ve dayanıklılığını arttıracak alternatif stratejilerden olabileceği sonucuna ulaşılmıştır.

Anahtar Kelimeler

• antosiyaninler
• mısır
• fizyoloji
• ön uygulama
• prolin

Etik Beyan

Bu makale insan veya hayvan denekleri üzerinde herhangi bir deney yapılmadığından etik onay gerektirmez.

Kaynakça

1. Ahmad, M., Shah, A., Sun, J., & Song, Y. (2020). Comparative study on leaf gas exchange, growth, grain yield, and water use efficiency under irrigation regimes for two maize hybrids. Agriculture, 10(169), 1-16. https://doi.org/10.3390/agriculture10090369.
2. Ahmed, M., Qadeer, U., Ahmed, Z. I., & Hassan, F. (2016). Improvement of wheat (Triticum aestivum) drought tolerance by seed priming with silicon. Arch. Agron. Soil Sci., 62(3), 299-315. https://doi.org/210.1080/03650340.03652015.01048235. https://www.tandfonline.com/doi/abs/10.1080/03650340.2015.1048235
3. Ali, Z., Hussein, M., & El-Taher, A. (2015). Effect of antioxidants on some morphological and anatomical features of maize grown under salinity conditions. International Journal of ChemTech Research, 8(6), 389-400. https://sphinxsai.com/2015/ch_vol2018_no2016/2011/(2389-2400)V2018N2016CT.pdf.
4. Amanullah, Y., Khalil, S., Jan, M., & Khan, A. (2009). Phenology, growth, and grain yield of maize as influenced by foliar applied urea at different growth stages. Journal of Plant Nutrition, 33(1), 71-79. https://doi.org/10.1080/01904160903391099.
5. Amin, M., Ahmad, R., Ali, A., Hussain, I., Mahmood, R., Aslam, M., & Lee, D. J. (2018). Influence of silicon fertilization on maize performance under limited water supply. Silicon, 10(2), 177-183. https://doi.org/110.1007/s12633-12015-19372-x.
6. Amin, M., Ahmad, R., Basra, S., & Murtaza, G. (2014). Silicon induced improvement in morpho-physiological traits of maize (Zea mays L.) under water deficit. Pak. J. Agric. Sci., 51(1), 187-196. http://www.pakjas.com.pk/papers/2260.pdf.
7. Biju, S., Fuentes, S., & Gupta, D. (2017). Silicon improves seed germination and alleviates drought stress in lentil crops by regulating osmolytes, hydrolytic enzymes and antioxidant defense system. Plant Physiol Biochem, 119, 250-264. https://doi.org/210.1016/j.plaphy.2017.1009.1001.
8. Cervilla, L., Blasco, B., Rios, J., Rosales, M., Sánchez-Rodríguez, E., Rubio-Wilhelmi, M., Romero, L., & Ruiz Juan, M. (2012). Parameters symptomatic for boron toxicity in leaves of tomato plants. Journal of Botany, 2012, 1-17 https://www.hindawi.com/journals/jb/2012/726206/.

9. Chen, D., Wang, S., Yin, L., & Deng, X. (2018). Howdoes silicon mediate plant water uptake and loss under water deficiency? Front. Plant Science, 9(281), 1-8. https://doi.org/10.3389/fpls.2018.00281.
10. Chen, W., Yao, X., Cai, K., & Chen, J. (2011). Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption. Biol. Trace Elem. Res., 142(1), 67-76. https://doi.org/10.1007/s12011-12010-18742-x.
11. Cuong, T. X., Ullah, H., Datta, A., & Hanh, T. C. (2017). Effects of silicon-based fertilizer on growth, yield and nutrient uptake of rice in tropical zone of Vietnam. Rice Sci, 24(5), 283-290. https://doi.org/210.1016/j.rsci.2017.1006.1002.
12. Dann, E., & Le, D. (2017). Effects of silicon amendment on soilborne and fruit diseases of avocado. Plants, 6(4), 2-15. https://doi.org/10.3390/plants6040051.
13. Demirok, A., & Tuylu, G. (2019). Effect of Drip and Subsurface Drip Irrigation on Maize Yield. Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 5(1), 16-31. https://doi.org/10.28979/comufbed.552158.
14. Dimitrios, S., & Georgia, N. (2015). Biostimulant activity of silicon in horticulture. Scientia Horticulturae, 2015(196), 66-81. https://doi.org/10.1016/j.scienta.2015.1009.1010.
15. Epstein, E. (1999). Annual review of plant physiology and molecular biology. Silicon, 50, 641–664 https://www.scirp.org/(S(czeh642tfqyw642orz553k641w640r645))/reference/ReferencesPapers.aspx?ReferenceID=1818347.
16. Foyer, C. (2018). Reactive oxygen species, oxidative signaling and the regulation of photosynthesis. Environmental and experimental botany, 154(2018), 134-142. https://doi.org/110.1016/j.envexpbot.2018.1005.1003.
17. Gao, X., Zou, C., Wang, L., & Zhang, F. (2006). Silicon decreases transpiration rate and conductance from stomata of maize plants. J. Plant Nutr., 29 1637-1647 https://www.tandfonline.com/doi/full/1610.1080/01904160600851494.
18. George, S., Manoharan, D., Li, J., Britton, M., & Parida, A. (2018). Drought and salt stress in Macrotyloma uniflorum leads to common and specific transcriptomic responses and reveals importance of raffinose family oligosaccharides in stress tolerance. Gene Reports, 10(2018), 7-16. https://doi.org/10.1016/j.genrep.2017.1010.1006.
19. Gong, H., Zhu, X., Chen, K., Wang, S., & Zhang, C. (2005). Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant science, 169(2), 313-321. https://doi.org/310.1016/j.plantsci.2005.1002.1023.
20. Guendouz, A., Semcheddine, N., Moumeni, L., & Hafsi, M. (2016). The effect of supplementary irrigation on leaf area, specific leaf weight, grain yield and water use efficiency in durum wheat (Triticum durum Desf.) cultivars. Ekin Journal of Crop Breeding and Genetics, 2(1), 82-89. https://dergipark.org.tr/en/pub/ekinjournal/issue/22787/243191.
21. Guerriero, G., Hausman, J. F., & Legay, S. (2016). Silicon and the plant extracellular matrix. Front. Plant Sci., 7(463), 1-8. https://doi.org/10.3389/fpls.2016.00463.
22. Hasan, S. A., Rabei, S. H., Nada, R. M., & Abogadallah, G. M. (2017). Water use efficiency in the drought-stressed sorghum and maize in relation to expression of aquaporin genes. Biologia Plantarum, 61(1), 127-137. https://doi.org/110.1007/s10535-10016-10656-10539.
23. He, C., Wang, L., Liu, J., Liu, X., Li, X., & Ma, J. (2013). Evidence for’silicon’ with in the cell walls of suspension – culture drice cells. New Phytol., 200, 700–709. https://doi.org/710.1111/nph.12401.
24. Horuz, A. (2016). The effects of silicon on plant development. Toprak Bilimi ve Bitki Besleme Dergisi, 6(2), 151-163. https://dergipark.org.tr/en/pub/tbbbd/issue/41828/508703.
25. Keenan, T., Sabate, S., & Gracia, C. (2010). The importance of mesophyll conductance in regulating forest ecosystem productivity during drought periods. Global Change Biol 16, 1019–1034. https://doi.org/1010.1111/j.1365-2486.2009.02017.x.
26. Khalil, F., Naiyan, X., Tayyab, M., & Pinghua, C. (2018). Screening of EMS-induced drought-tolerant sugarcane mutants employing physiological, molecular and enzymatic approaches. Agronomy, 8(10), 2-13. https://doi.org/10.3390/agronomy8100226.
27. Khampas, S., Lertrat, K., Lomthaisong, K., & Suriharn, B. (2013). Variability in phytochemicals and antioxidant activity in corn at immaturity and physiological maturity stages. International Food Research Journal, 20(6), 3149-3157. http://www.ifrj.upm.edu.my/3120%3120(3106)%202013/202025%202020IFRJ%202020%202020(202006)%202013%202020Bhalang%220221.pdf.
28. Kidd, P. S., Llugany, M., Poschenrieder, C., Gunse, B., & Barcelo, J. (2001). The role of root exudates in aluminium resistance and silicon-induced amelioration of aluminium toxicity in three varieties of maize (Zea mays L.). J. Exp. Bot 52, 1339-1352. https://doi.org/1310.1093/jexbot/1352.1359.1339.
29. Lee, S. K., Sohn, E. Y., Hamayun, M., Yoon, J. Y., & Lee, I. J. (2010). Effect of silicon on growth and salinity stress of soybean plant grown under hydroponic system. Agrofor. Syst., 80 333–340. https://doi.org/310.1007/s10457-10010-19299-10456.
30. Lesk, C., Rowhani, P., & Ramankutty, N. (2016). Influence of extreme weather disasters on global crop production. Nature, 529(7584), 84-87. https://doi.org/10.1038/nature16467.
31. Liang, Y., Chen, Q., Liu, Q., Zhang, WH , & Ding, R. (2003). Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J. Plant Physiol. , 160, 1157–1164. https://doi.org/1110.1078/0176-1617-01065.
32. Liang, Y., Hua, H., Zhu, Y., Zhang, J., Cheng, C., & Romheld, V. (2006). Importance of plant species and external silicon concentration to active silicon uptake and transport. New Phytologist, 172(1), 63-72. https://doi.org/10.1111/j.1469-8137.2006.01797.x.
33. Ma, J. F., & Yamaji, N. (2015). A cooperative system of silicon transport in plants. Trends Plant Sci. , 20, 435–442. https://doi.org/410.1016/j.tplants.2015.1004.1007.
34. Madhava, R. K., & Sresty, T. V. S. (2000). Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant science, 157(1), 113-128. https://doi.org/110.1016/S0168-9452(1000)00273-00279.
35. Öktem, A., Şimsek, M., & Öktem, A. (2003). Deficit irrigation effects on sweet corn (Zea mays saccharata Sturt) with drip irrigation system in a semi-arid region: I. Water-yield relationship. Agricultural Water Management, 61(1), 63-74. https://doi.org/10.1016/S0378-3774(1002)00161-00160.
36. Özdemir, E. (2021). Silicon stimulated bioactive and physiological metabolisms of purple corn (Zea mays indentata L.) under deficit and well-watered conditions. 3 Biotech, 11(319), 1-13. https://doi.org/10.1007/s13205-13021-02873-x.
37. Pang, Z., Tayyab, M., Islam, W., Tarin, M., Sarfaraz, R., Naveed, H., Zaman, S., Zhang, B., Yuan, Z., & Zhang, H. (2019). Silicon mediated improvement in tolerance of economically important crops under drought stress. Appl Ecol Environ Res, 17(3), 6151-6170. http://dx.doi.org/6110.15666/aeer/11703_61516170.
38. Parveen, A., Liu, W., Hussain, S., Asghar, J., Perveen, S., & Xiong, Y. (2019). Silicon priming regulates morpho-physiological growth and oxidative metabolism in maize under drought stress. Plants, 8(431), 1-14. https://doi.org/10.3390/plants8100431.
39. Pei, Z. F., Ming, D. F., Liu, D., Wan, G. L., Geng, X. X., Gong, H. J., & Zhou, W. J. (2010). Silicon improves the tolerance to water-deficit stress induced by polyethylene glycol in wheat (Triticum aestivum L.) seedlings. J. Plant Growth Regul. , 29, 106-115. https://doi.org/110.1007/s00344-00009-09120-00349
40. Rizwan, M., Ali, S., Abbas, T., Adrees, M., Zia-ur-Rehman, M., Ibrahim, M., Abbas, F., Qayyum, M., & Nawaz, R. (2018). Residual effects of biochar on growth, photosynthesis and cadmium uptake in rice (Oryza sativa L.) under Cd stress with different water conditions. Journal of Environmental Management, 206(2018), 676-683. https://doi.org/610.1016/j.jenvman.2017.1010.1035.
41. Sade, B., Soylu, S., & Yetim, E. (2011). Drought and oxidative stress. African Journal of Biotechnology, 10(54), 11102-11109. DOI: 11110.15897/AJB11111.11564.
42. Seymen, M. (2021). Comparative analysis of the relationship between morphological, physiological, and biochemical properties in spinach (Spinacea oleracea L.) under deficit irrigation conditions. Turkish Journal of Agriculture and Forestry, 45(1), 55-67. https://doi.org/10.3906/tar-2004-3979.
43. Smirnoff, N., & Cumbes, Q. J. (1989). Hydroxyl radicals scavenging activity of compatible solutes. Phytochem 828, 1057–1060. http://dx.doi.org/1010.1016/0031-9422(1089)80182-80187.
44. Şahin, M., & Al-Bayati, Y. (2018). Konya ili açık tarla koşullarında marul bitkisinin su-verim parametrelerinin belirlenmesi. Toprak Su Dergisi, 7(2), 38-45. https://doi.org/10.21657/topraksu.460725.
45. Tayyab, M., Islam, W., Khalil, F., Ziqin, P., Caifang, Z., Arafat, Y., Hui, L., Rizwan, M., Ahmad, K., & Waheed, S. (2018). Biochar: An efficient way to manage low water availability in plants. Appl. Ecol. Environ. Res, 16(3), 2565-2583. http://dx.doi.org/2510.15666/aeer/11603_25652583
46. Ullah, H., Luc, P., Gautam, A., & Datta, A. (2018). Growth, yield and silicon uptake of rice (Oryza sativa) as influenced by dose and timing of silicon application under water-deficit stress. Arch Agron Soil Sci. , 64, 318–330. https://doi.org/310.1080/03650340.03652017.01350782.
47. Verma, K., Song, X., Lin, B., Guo, D., Singh, M., Rajput, V., Singh, R., Singh, P., Sharma, A., Malviya, M., Chen, G., & Li, Y. (2021). Silicon induced drought tolerance in crop plants: physiological adaptation strategies. Silicon, 14(6), 2473-2487. https://doi.org/2410.1007/s12633-12021-01071-x.
48. Warner, K., & Jones, G. (2017). A population-induced renewable energy timeline in nine world regions. Energy Policy, 101(2017), 65-76. https://doi.org/10.1016/j.enpol.2016.1011.1031.
49. Wu, S., Hu, C., Tan, Q., Li, L., Shi, K., Zheng, Y., & Sun, X. (2015). Drought stress tolerance mediated by zinc-induced antioxidative defense and osmotic adjustment in cotton (Gossypium hirsutum). Acta Physiol. Plant. , 37(167), 2-9. https://doi.org/10.1007/s11738-11015-11919-11733.
50. Yavuz, D., Seymen, M., Yavuz, N., Çoklar, H., & Ercan, M. (2021). Effects of water stress applied at various phenological stages on yield, quality, and water use efficiency of melon. Agricultural Water Management, 246(2021), 1-11. https://doi.org/10.1016/j.agwat.2020.106673
51. Yin, L., Wang, S., Li, J., Tanaka, K., & Oka, M. (2013). Application of silicon improves salt tolerance through ameliorating osmotic and ionic stresses in the seedling of Sorghum bicolor. Acta Physiol. Plant., 35, 3099–3107. https://doi.org/3010.1007/s11738-11013-11343-11735.