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Effects of microwave radiation to the bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae)

Yıl 2025, Cilt: 30 Sayı: 2, 503 - 513, 21.08.2025
https://doi.org/10.37908/mkutbd.1627805

Öz

Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae) is a pest that causes economic losses particularly on dry beans. Various control methods, especially those involving chemical treatments, have been successfully used against this pest. However, excessive use of chemicals poses resistance problems in insects and also threatens environmental and human health. For this reason, producers and researchers have been turning toward alternative control methods. One such method is the use of microwave radiation. This technique results in minimal product loss while significantly reducing pest populations. In this study, the effects of different microwave levels on A. obtectus, a storage pest of beans, were determined. In the trials, 7 different exposure times (5, 10, 20, 40, 60, 80, and 100 seconds) and 5 different power levels (120, 350, 460, 600, and 700 watts) were applied to determine the mortality rate of the pest. The number of pest deaths was recorded. The laboratory production of this pest and the experiments were conducted in a room at 25±1 °C and 60±5% relative humidity. The results showed that mortality rates increased both with longer exposure to low radiation levels (120 watt, 100 sec., 73%; 350 watt, 60 sec., 100%) and with shorter exposure to high radiation levels (700 watt, 5 sec., 91%; 10 sec, 95%; 20 sec., 100%). The findings suggest that this method can be recommended as an alternative approach for controlling A. obtectus.

Kaynakça

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  • Abd El-Raheem, A.M., & Said, S.M. (2016). Does microwave radiation have an effect on stored product insects and their host food quality?. Academic Journal of Entomology, 9 (4), 51-61. https://doi.org/10.5829/idosi.aje.2016.9.4.112151
  • Adler, C. (2004). Integrated stored product protection methods to replace the use of methyl bromide for pest control in grains, dried fruits and nuts. In Proceedings of International Conference on Alternatives to Methyl Bromide (p. 227-236).
  • Agha, W.N.A., Amin, A.H., Khidr, S.K., & Ismail, A.Y. (2017). Entomocidal activity of microwave energy & some aqueous plant extracts against Tribolium castaneum Herbst & Trogoderma granarium Everts. In AIP Conference Proceedings (Vol. 1888, No. 1, 1-13). AIP Publishing. https://doi.org/10.1063/1.5004282
  • Ahmed, M. (2001). Disinfestation of stored grains, pulses, dried fruits and nuts, and other dried foods. Food Irradiation Principles and Applications, Wiley, New York, 77-112.
  • Alvarez, N., McKey, D., Hossaert‐Mckey, M., Born, C., Mercier, L., & Benrey, B. (2005). Ancient and recent evolutionary history of the bruchid beetle, Acanthoscelides obtectus Say, a cosmopolitan pest of beans. Molecular Ecology, 14 (4), 1015-1024. https://doi.org/10.1111/j.1365-294X.2005.02470.x
  • Arthur, F.H. (1996). Grain protectants: Current status and prospects for the future. Journal of Stored Products Research, 32 (4), 293-302. https://doi.org/10.1016/S0022-474X(96)00033-1
  • Athanassiou, C.G., & Arthur, F.H. (Eds.). (2018). Recent advances in stored product protection (p. 273). Berlin, Germany: Springer.
  • Ayvaz, A., & Karabörklü, S. (2008). Effect of cold storage and different diets on Ephestia kuehniella Zeller (Lep: Pyralidae). Journal of Pest Science, 81, 57-62. https://doi.org/10.1007/s10340-008-0192-2
  • Ayvaz, A., Albayrak, S., & Karabörklü, S. (2008). Gamma radiation sensitivity of the eggs, larvae and pupae of Indian meal moth Plodia interpunctella (Hübner)(Lepidoptera: Pyralidae). Pest Management Science: formerly Pesticide Science, 64 (5), 505-512. https://doi.org/10.1002/ps.1526
  • Ayvaz, A., Albayrak, S., & Tunçbilek, A.Ş. (2007). Inherited sterility in Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae): Effect of gamma radiation on insect fecundity, fertility and developmental period. Journal of Stored Products Research, 43 (3), 234-239. https://doi.org/10.1016/j.jspr.2006.06.003
  • Azizoglu, U., Yilmaz, S., Karabörklü, S., & Ayvaz, A. (2010). Ovicidal activity of microwave and UV radiations on Mediterranean flour moth Ephestia kuehniella Zeller, 1879 (Lepidoptera: Pyralidae). Turkish Journal of Entomology, 35 (3), 437-446. https://dergipark.org.tr/en/pub/entoted/issue/5692/969296
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Mikrodalga radyasyonunun fasulye tohum böceği Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae) üzerindeki etkileri

Yıl 2025, Cilt: 30 Sayı: 2, 503 - 513, 21.08.2025
https://doi.org/10.37908/mkutbd.1627805

Öz

Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae) özellikle kuru fasulye üzerinde ekonomik kayba neden olan bir zararlıdır. Bu zararlı ile mücadelede özellikle kimyasalların tercih edildiği farklı yöntemler kullanılarak başarılı sonuçlar elde edilmektedir. Ancak yoğun kimyasal kullanımı böceklerde direnç probleminin yanında çevre ve insan sağlığı için de tehdit oluşturmaktadır. Bu sebeple üreticiler ve araştırıcılar alternatif mücadele yöntemlerine yönelmektedir. Bu yöntemlerden birisi de mikrodalga radyasyonu kullanılarak yapılan mücadeledir. Bu yöntem kullanıldığında üründe minimum kayıp meydana gelirken, zararlıların popülasyonunu oldukça düşük seviyelere inmektedir. Bu çalışmada bir depo zararlısı olan fasulye tohum böceği A. obtectus üzerinde farklı mikrodalga değerlerinin etkisi belirlenmiştir. Denemelerde zararlının ölüm oranını belirlemek amacıyla 7 farklı sürede (5, 10, 20, 40, 60, 80, 100 saniye), 5 farklı güç seviyesi (120, 350, 460, 600, 700 watt) uygulanmıştır. Zararlının ölüm miktarı ortaya konmuştur. Bu zararlının laboratuvar koşullarındaki üretimi ve denemeler 25±1 °C sıcaklık ve %60±5 orantılı neme sahip odada gerçekleştirilmiştir. Sonuçlara göre hem düşük radyasyon değerine uzun süre maruz kalma (120 watt, 100 sn, %73; 350 watt, 60 sn, %100) durumunda hem de yüksek radyasyon değerine kısa süre maruz kalma durumunda (700 watt, 5 sn, %91; 10 sn, %95; 20 sn, %100) ölüm oranının arttığı gözlenmiştir. Sonuçlar bu yöntemin A. obtectus mücadelesinde alternatif bir yöntem olarak önerilebileceğini göstermiştir.

Kaynakça

  • Abate, T., & Ampofo, J.K.O. (1996). Insect pests of beans in Africa: Their ecology and management. Annual Review of Entomology, 41 (1), 45-73. https://doi.org/10.1146/annurev.en.41.010196.000401
  • Abd El-Raheem, A.M., & Said, S.M. (2016). Does microwave radiation have an effect on stored product insects and their host food quality?. Academic Journal of Entomology, 9 (4), 51-61. https://doi.org/10.5829/idosi.aje.2016.9.4.112151
  • Adler, C. (2004). Integrated stored product protection methods to replace the use of methyl bromide for pest control in grains, dried fruits and nuts. In Proceedings of International Conference on Alternatives to Methyl Bromide (p. 227-236).
  • Agha, W.N.A., Amin, A.H., Khidr, S.K., & Ismail, A.Y. (2017). Entomocidal activity of microwave energy & some aqueous plant extracts against Tribolium castaneum Herbst & Trogoderma granarium Everts. In AIP Conference Proceedings (Vol. 1888, No. 1, 1-13). AIP Publishing. https://doi.org/10.1063/1.5004282
  • Ahmed, M. (2001). Disinfestation of stored grains, pulses, dried fruits and nuts, and other dried foods. Food Irradiation Principles and Applications, Wiley, New York, 77-112.
  • Alvarez, N., McKey, D., Hossaert‐Mckey, M., Born, C., Mercier, L., & Benrey, B. (2005). Ancient and recent evolutionary history of the bruchid beetle, Acanthoscelides obtectus Say, a cosmopolitan pest of beans. Molecular Ecology, 14 (4), 1015-1024. https://doi.org/10.1111/j.1365-294X.2005.02470.x
  • Arthur, F.H. (1996). Grain protectants: Current status and prospects for the future. Journal of Stored Products Research, 32 (4), 293-302. https://doi.org/10.1016/S0022-474X(96)00033-1
  • Athanassiou, C.G., & Arthur, F.H. (Eds.). (2018). Recent advances in stored product protection (p. 273). Berlin, Germany: Springer.
  • Ayvaz, A., & Karabörklü, S. (2008). Effect of cold storage and different diets on Ephestia kuehniella Zeller (Lep: Pyralidae). Journal of Pest Science, 81, 57-62. https://doi.org/10.1007/s10340-008-0192-2
  • Ayvaz, A., Albayrak, S., & Karabörklü, S. (2008). Gamma radiation sensitivity of the eggs, larvae and pupae of Indian meal moth Plodia interpunctella (Hübner)(Lepidoptera: Pyralidae). Pest Management Science: formerly Pesticide Science, 64 (5), 505-512. https://doi.org/10.1002/ps.1526
  • Ayvaz, A., Albayrak, S., & Tunçbilek, A.Ş. (2007). Inherited sterility in Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae): Effect of gamma radiation on insect fecundity, fertility and developmental period. Journal of Stored Products Research, 43 (3), 234-239. https://doi.org/10.1016/j.jspr.2006.06.003
  • Azizoglu, U., Yilmaz, S., Karabörklü, S., & Ayvaz, A. (2010). Ovicidal activity of microwave and UV radiations on Mediterranean flour moth Ephestia kuehniella Zeller, 1879 (Lepidoptera: Pyralidae). Turkish Journal of Entomology, 35 (3), 437-446. https://dergipark.org.tr/en/pub/entoted/issue/5692/969296
  • Bağcı, F., Yılmaz, A., & Ertürk, S. (2014). Ankara ili hububat depolarında bulunan zararlı böcek türleri. Plant Protection Bulletin, 54 (1), 69-78. https://dergipark.org.tr/tr/pub/bitkorb/issue/28081/383002
  • Baier, A.H., & Webster, B.D. (1992). Control of Acanthoscelides obtectus Say (Coleoptera: Bruchidae) in Phaseolus vulgaris L. seed stored on small farms—I. Evaluation of damage. Journal of Stored Products Research, 28 (4), 289-293. https://doi.org/10.1016/0022-474X(92)90011-E
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  • Daglish, G.J., Nayak, M.K., Arthur, F.H., & Athanassiou, C.G. (2018). Insect pest management in stored grain. Recent Advances in Stored Product Protection, 45-63. https://doi.org/10.1007/978-3-662-56125-6_3
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  • Donahaye, E.J. (2000). Current status of non-residual control methods against stored product pests. Crop Protection, 19 (8-10), 571-576. https://doi.org/10.1016/S0261-2194(00)00074-0
  • Donahaye, E.J., & Messer, E. (1992). Reduction in grain storage losses of small-scale farmers in tropical countries. In Research Report RR-91-7, the Allan Shawn Feinstein World Hunger Program. Brown University USA.
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  • Fields, P.G. (1992). The control of stored-product insects and mites with extreme temperatures. Journal of Stored Products Research, 28 (2), 89-118. https://doi.org/10.1016/0022-474X(92)90018
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  • Hagstrum, D.W., & Flinn, P.W. (2014). Modern stored-product insect pest management. Journal of Plant Protection Research, 54 (3), 205-210. http://hdl.handle.net/2097/20502
  • Hagstrum, D.W., & Subramanyam, Bh. (2006). Fundamentals of stored-product entomology. AACC International, St. Paul, MN.
  • Halverson, S.L., Phillips, T.W., Bigelow, T.S., Mbata, G.N., & Payton, M.E. (1999). The control of various species of stored-product insects with EHF energy. Proceeding of the Annual International Research Conference on Methyl Bromide Alternatives and Emissions Reductions, 541-544.
  • Hansen, L.S., & Jensen, K.M. (2002). Effect of temperature on parasitism and host-feeding of Trichogramma turkestanica (Hymenoptera: Trichogrammatidae) on Ephestia kuehniella (Lepidoptera: Pyralidae). Journal of Economic Entomology, 95 (1), 50-56. https://doi.org/10.1603/0022-0493-95.1.50
  • Harein, P.K., & Soderstrom, E.L. (1966). Coleoptera infesting stored products. In Insect colonization and mass production (pp. 241-257). Academic Press.
  • Jackson, M.A., & Jaronski, S.T. (2009). Production of microsclerotia of the fungal entomopathogen Metarhizium anisopliae and their potential for use as a biocontrol agent for soil-inhabiting insects. Mycological Research, 113 (8), 842-850. https://doi.org/10.1016/j.mycres.2009.03.004
  • Karadağ, E., & Kayahan, A. (2021). Effect of microwave radiation on flour beetle, Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae). Bilecik Seyh Edebali University Journal of Science, 8 (1), 186-192. https://doi.org/10.35193/bseufbd.870009
  • Kaya, K., Sertkaya, E., Üremiş, İ., & Soylu, S. (2018). Determination of chemical composition and fumigant insecticidal activities of essential oils of some medicinal plants against the adults of cowpea weevil, Callosobruchus maculatus. KSÜ Tarım ve Doğa Dergisi, 21 (5), 708-714. https://doi.org/10.18016/ksudobil.386176
  • Koca, A.S., & Yılmaz, A. (2025). Insecticidal efficiency of green-synthesized zinc oxide nanoparticles on bean weevil, Acanthoscelides obtectus Say. (Coleoptera: Chrysomelidae). Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 30 (1), 237250. https://doi.org/10.37908/mkutbd.1589636
  • Kumar, C., Ram, C.L., Jha, S.N., & Vishwakarma, R.K. (2021). Warehouse storage management of wheat and their role in food security. Frontiers in Sustainable Food Systems, 5, 675626. https://doi.org/10.3389/fsufs.2021.675626
  • Lapidot, M., Saveanu, S., Padova, R., & Ross, I. (1991). Insect disinfestation by irradiation. Insect Disinfestation of Food and Agricultural Products by Irradiation, Vienna, 93-103. https://citeseerx.ist.psu.edu/document
  • Manickavasagan, A., Alahakoon, P.M.K., Al-Busaidi, T.K., Al-Adawi, S., Al-Wahaibi, A.K., Al-Raeesi, A.A., Al-Yahyai, R., & Jayas, D.S. (2013). Disinfestation of stored dates using microwave energy. Journal of Stored Products Research, 55, 1-5. https://doi.org/10.1016/j.jspr.2013.05.005
  • Massa, R., Panariello, G., Pinchera, D., Schettino, F., Caprio, E., Griffo, R., & Migliore, M.D. (2017). Experimental and numerical evaluations on palm microwave heating for Red Palm Weevil pest control. Scientific Reports, 7 (1), 45299. https://doi.org/10.1038/srep45299
  • Minitab (2010). Minitab Inc. MINITAB: Minitab for Windows Version Release 17. State College, PA, USA. Moore, D., & Prior C. (1993). The potential of mycoinsecticides. Biocontrol News and Information, 14, 31-40. https://www.cabidigitallibrary.org/doi/full/10.5555/19931170567
  • Mutungi, C., Affognon, H.D., Njoroge, A.W., Manono, J., Baributsa, D., & Murdock, L.L. (2015). Triple-layer plastic bags protect dry common beans (Phaseolus vulgaris) against damage by Acanthoscelides obtectus (Coleoptera: Chrysomelidae) during storage. Journal of Economic Entomology, 108 (5), 2479-2488. https://doi.org/10.1093/jee/tov197
  • Njoroge, A.W., Affognon, H., Mutungi, C., Richter, U., Hensel, O., Rohde, B., & Mankin, R.W. (2017). Bioacoustics of Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) on Phaseolus vulgaris (Fabaceae). Florida Entomologist, 100 (1), 109-115. https://doi.org/10.1653/024.100.0116
  • Patil, H., Shejale, K. P., Jabaraj, R., Shah, N., & Kumar, G. (2020). Disinfestation of red flour beetle (Tribolium castaneum) present in almonds (Prunus dulcis) using microwave heating and evaluation of quality and shelf life of almonds. Journal of Stored Products Research, 87, 101616. https://doi.org/10.1016/j.jspr.2020.101616
  • Pei, Y., Tao, T., Yang, G., Wang, Y., Yan, W., & Ding, C. (2018). Lethal effects and mechanism of infrared radiation on Sitophilus zeamais and Tribolium castaneum in rough rice. Food Control, 88, 149-158. https://doi.org/10.1016/j.foodcont.2018.01.012
  • Rees, D. (2004). Insects of stored products. CSIRO Publishing, Collingwood, Australia. Regnault-Roger, C. (1997). The potential of botanical essential oils for insect pest control. Integrated Pest Management Reviews, 2, 25-34. https://doi.org/10.1023/A:1018472227889
  • Sadeghi, R., Mirabi Moghaddam, R., & Seyedabadi, E. (2018). Microwave use in the control of Ephestia kuehniella (Lepidoptera: Pyralidae) in dried fig and raisin and effects on fruit sensory characteristics. Journal of Economic Entomology, 111 (3), 1177-1179. https://doi.org/10.1093/jee/toy070
  • Schmale, I., Wäckers, F.L., Cardona, C., & Dorn, S. (2002). Field infestation of Phaseolus vulgaris by Acanthoscelides obtectus (Coleoptera: Bruchidae), parasitoid abundance, and consequences for storage pest control. Environmental Entomology, 31 (5), 859-863. https://doi.org/10.1603/0046-225X-31.5.859
  • Schöller, M., Prozell, S., Al-Kirshi, A.G., & Reichmuth, C. (1997). Towards biological control as a major component of integrated pest management in stored product protection. Journal of Stored Products Research, 33 (1), 81-97. https://doi.org/10.1016/S0022-474X(96)00048-3
  • Sertkaya, E. (2013). Fumigant toxicity of the essential oils from medicinal plants against bean weevil, Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Asian Journal of Chemistry, 25, 553-555. Sinha, R.N., & Watters, F.L. (1985). Insect pests of flour mills, grain elevators, and feed mills and their control (No. 1776, 290 pp).
  • Soares, M.A., Quintela, E.D., Mascarin, G.M., & Arthurs, S.P. (2015). Effect of temperature on the development and feeding behavior of Acanthoscelides obtectus (Chrysomelidae: Bruchinae) on dry bean (Phaseolus vulgaris L.). Journal of Stored Products Research, 61, 90-96. https://doi.org/10.1016/j.jspr.2014.12.005
  • SPSS. (2001). SPSS Version 10.0. SPSS Inc, 233 S. Wacker Drive, Chicago, Illinois.
  • Srivastava, S., & Mishra, H.N. (2022). Disinfestation of Rhyzopertha dominica Coleoptera: Bostrichidae by combinational approach of microwave ultraviolet and vacuum assisted process in stored rice grains. International Journal of Tropical Insect Science, 42, 1535-1542. https://doi.org/10.1007/s42690-021-00672-8
  • Strong, R.G., Partida, G.J., & Warner, D.N. (1968). Rearing stored-product insects for laboratory studies: bean and cowpea weevils. Journal of Economic Entomology, 61 (3), 747-751. https://doi.org/10.1093/jee/61.3.747
  • Taheri, S., McFarlane, D.J., Mattner, S.W., & Brodie, G.I. (2022). Potential of microwave heating and plasma for biosecurity applications. Thermo, 2 (3), 312-333. https://doi.org/10.3390/thermo2030022
  • Toews, M.D., & Subramanyam, B. (2003). Contribution of contact toxicity and wheat condition to mortality of stored‐product insects exposed to spinosad. Pest Management Science, 59 (5), 538-544. https://doi.org/10.1002/ps.660
  • Tuda, M. (2007). Applied evolutionary ecology of insects of the subfamily Bruchinae (Coleoptera: Chrysomelidae). Applied Entomology and Zoology, 42 (3), 337-346. https://doi.org/10.1303/aez.2007.337
  • Vadivambal, R., Jayas, D.S., & White, N.D.G. (2007). Wheat disinfestation using microwave energy. Journal of Stored Products Research, 43 (4), 508-514. https://doi.org/10.1016/j.jspr.2007.01.007
  • Vadivambal, R., Jayas, D.S., & White, N.D.G. (2008). Determination of mortality of different life stages of Tribolium castaneum (Coleoptera: Tenebrionidae) in stored barley using microwaves. Journal of Economic Entomology, 101 (3), 1011-1021. https://doi.org/10.1093/jee/101.3.1011
  • Velten, G., Rott, A.S., Petit, B.J.C., Cardona, C., & Dorn, S. (2008). Improved bruchid management through favorable host plant traits and natural enemies. Biological Control, 47 (2), 133-140. https://doi.org/10.1016/j.biocontrol.2008.07.009
  • Vincent, C., Hallman, G., Panneton, B., & Fleurat-Lessard, F. (2003). Management of agricultural insects with physical control methods. Annual Review of Entomology, 48 (1), 261-281. https://doi.org/10.1146/annurev.ento.48.091801.112639
  • Wang, S., & Tang, J. (2001). Radio frequency and microwave alternative treatments for insect control in nuts: A review. Agricultural Engineering Journal, 10 (3&4), 105-120. https://public.wsu.edu/~sjwang/review-rf-insect.pdf
  • Yadav, D.N., Anand, T., Sharma, M., & Gupta, R.K. (2014). Microwave technology for disinfestation of cereals and pulses: An overview. Journal of Food Science and Technology, 51, 3568-3576. https://doi.org/10.1007/s13197-012-0912-8
  • Yanagawa, A., Kajiwara, A., Nakajima, H., Desmond-Le Quéméner, E., Steyer, J.P., Lewis, V., & Mitani, T. (2020). Physical assessments of termites (Termitidae) under 2.45 GHz microwave irradiation. Scientific Reports, 10 (1), 5197. https://doi.org/10.1038/s41598-020-61902-6
  • Yankova, V., & Sofkova, S. (2013). Possibilities for control of bean weevil (Acanthoscelides obtectus Say) in common bean (Phaseolus vulgaris L.) in open field conditions. Annual Report of the Bean Improvement Cooperative, 56, 97-98. https://www.researchgate.net_A. obtectus
  • Zettler, J.L., & Arthur, F.H. (2000). Chemical control of stored product insects with fumigants and residual treatments. Crop Protection, 19 (8-10), 577-582. https://doi.org/10.1016/S0261-2194(00)00075-2
Toplam 67 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Koruma (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Betül Kayahan 0000-0001-6000-0780

Ali Kayahan 0000-0002-3671-254X

Erken Görünüm Tarihi 9 Ağustos 2025
Yayımlanma Tarihi 21 Ağustos 2025
Gönderilme Tarihi 27 Ocak 2025
Kabul Tarihi 2 Mayıs 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 30 Sayı: 2

Kaynak Göster

APA Kayahan, B., & Kayahan, A. (2025). Effects of microwave radiation to the bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae). Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 30(2), 503-513. https://doi.org/10.37908/mkutbd.1627805

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