Investigation of the effects of lipoic acid and dihydrolipoate on experimental renal ischemia-reperfusion model

Öz

Objective: Ischemic/reperfusion (I/R) causes tissue injury and the leading cause of acute kidney injury. In this study, we aimed to investigate the effects of the long and short-term usage of ALA and short-term DHLA on oxidative stress markers in the experimental renal ischemia-reperfusion model. Methods: Forty male rats (250 to 300 gr) were divided into 5 groups: control; I/R group; long-term ALA+IR group; short-term ALA+IR group; and short-term DHLA+IR group. Ischemia was carried out for 45 minutes followed by reperfusion for 4 hours. Thiobarbituric acid reactive sunstances (TBARM), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities in tissue samples and serum total antioxidant status (TAS) and total oxidative stress (TOS) assayed by the spectrophotometrically. Tissue samples were investigated by histopathological analyzes. Results: TBARM (Control: 0.38±0.05. I/R: 1.37±0.17, long-term ALA-treated group:1.025±0.15, short-term ALA-treated group: 0.68±0.09, short-term DHLA-treated group: 0.38±0.04 (nmol/mg protein); p<0,001) CAT (Control: 0.12±0.02, I/R: 0.04±0.008, long-term ALA-treated group: 0.07±0.01, short-term ALA-treated group:0.06±0.008, short-term DHLA-treated group: 0.08±0.01 (k/mg protein); p<0.001), GSH-Px (Control: 0.45±0.04, I/R: 0.21±0.028, long-term ALA-treated group: 0.37±0.05, short-term ALA-treated group :0.34±0.05, short-term DHLA-treated group: 0.37±0.04 (U/mg protein); p<0.001), and serum OSI levels (Control: 1.32±0.15, I/R: 3.08±0.44, long-term ALA-treated group: 1.775±0.21, short-term ALA-treated group: 1.85±0.37, short-term DHLA-treated group: 1.53±0.21 (arbitrary unit) ; p<0.001) were improved in the long and short-term ALA-treated group and short-term DHLA-treated group compared to the I/R group. These findings were more prominent in histopathological tissue samples in the DHLA-treated group. Conclusion: We consider that both long-term and short-term ALA applications have the potential for the treatment of renal I/R damage. Besides, DHLA is more effective than ALA.

Anahtar Kelimeler

• Ischemic Damage
• Reperfusion
• Lipoic Acid
• Dihydrolipoat
• Antioxidant Enzyme

Proje Numarası

16341

Deneysel böbrek iskemi-reperfüzyon modelinde lipoik asit ve dihidrolipoat kullanımının etkilerinin incelenmesi

Öz

Amaç: İskemi/reperfüzyon (I/R) doku hasarına neden olarak akut böbrek hasarına yol açar. Bu çalışmada, deneysel böbrek iskemi-reperfüzyon modelinde uzun ve kısa süreli ALA ve kısa süreli DHLA kullanımının oksidatif stres belirteçleri üzerine etkilerinin araştırması amaçlanmıştır. . Yöntem: Kırk adet erkek rat (250-300 gr) 5 gruba ayrılmıştır: kontrol grubu; I/R grubu; uzun vadeli ALA+IR grubu; kısa vadeli ALA+IR grubu ve kısa vadeli DHLA+IR grubu. 45 dakika süreyle iskemi, ardından 4 saat süreyle reperfüzyon uygulanmıştır. Doku örneklerinde Tiyobarbitürik asid reaktif maddeler (TBARM), katalaz (CAT), süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) aktiviteleri ile total antioksidan durum (TAS) ve total oksidatif stres kapasitesi (TOS) spektrofotometrik olarak ölçülmüştür. Doku örnekleri ayrıca histopatolojik olarak analiz edilmiştir. Bulgular: Uzun ve kısa süreli ALA uygulanan grupta ve kısa süreli DHLA uygulanan grupta I/R grubuna kıyasla TBARM (Kontrol: 0.38±0.05, I/R: 1.37±0.17, uzun vadeli ALA+IR grubu: 1.025±0.15, kısa vadeli ALA+IR grubu: 0.68±0.09, kısa vadeli DHLA+IR grubu: 0,38±0,04 (nmol/mg protein); p<0.001), CAT (Kontrol: 0.12±0.02, I/R: 0.04±0.008, uzun vadeli ALA+IR grubu: 0,07±0,01, kısa vadeli ALA+IR grubu: 0,06±0,008, kısa vadeli DHLA+IR grubu: 0.08±0.01 (k/mg protein; p<0.001), GSH-Px (Kontrol: 0.45±0.04, I/R: 0.21±0.028, uzun vadeli ALA+IR grubu: 0.37±0.05, kısa vadeli ALA+IR grubu: 0.34±0.05, kısa vadeli DHLA+IR grubu: 0.37±0.04 (U/mg protein); p<0.001), ve serum OSI (Kontrol: 1.32±0.15, I/R: 3.08±0.44, uzun vadeli ALA+IR grubu: 1.775±0.21, kısa vadeli ALA+IR grubu: 1.85±0.37, kısa vadeli DHLA+IR grubu: 1.53±0.21 (arbitary unit); p<0.001) seviyelerinde iyileşme gözlemlenmiştir. Bu bulgular, histopatolojik doku örneklerinde DHLA uygulanan grupta daha belirgindir Sonuç: Hem uzun süreli hem de kısa süreli ALA uygulamalarının renal I/R hasarının tedavisi için potansiyele sahip olduğu düşünülmektedir. Ayrıca DHLA’nın, ALA’dan daha etkili olduğu düşünülmektedir.

Anahtar Kelimeler

• İskemik Hasar
• Reperfüzyon
• Lipoik Asit
• Dihidrolipoat
• Antioksidan Enzim

Destekleyen Kurum

Hatay Mustafa Kemal University Scientific Research Project Coordination Unit (Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Birimi)

Proje Numarası

16341

Teşekkür

Hatay Mustafa Kemal University Scientific Research Project Coordination Unit (Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Birimi)

Kaynakça

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