The Effect of Brotizolam Induced Appetite on Serum Ghrelin Levels in Mice

Öz

Objective: Appetite is affected by the metabolic profile, infections and nutritional shape in domestic animals. Regulation of appetite involves many complex physiological processes. Ghrelin is well known appetite-enhancing hormone. The appetite is also managed by the lateral hypothalamus. On the other hand, the ventromedial nucleus is most commonly associated with satiety. Brotizolam, member of the 1,4-benzodiazepines derivative, is blocking the activity of the ventromedial hypothalamus and used to increase appetite in domestic animals. The aim of this study was to investigate the impact of brotizolam on serum ghrelin levels, enhancer appetite hormone.Methods: A total of 16 mice were equally assigned to two groups as control (0.9% NaCl, IP) and brotizolam (2 μg/kg, IP). Blood samples was collected 30 min after the administration in both groups. Serum ghrelin levels were determined by enzyme linked immunoassay.Results: There was a significance differences between control and brotizolam groups (P<0.001). The results of the study showed that the administration of brotizolam increased serum ghrelin levels.Conclusion: Brotizolam, which suppresses ventromedial hypothalamus and satiety, can activate appetite by activation of the lateral hypothalamus and increase in ghrelin levels.In addition, ghrelin may be used as an endocrine marker of appetite.

Anahtar Kelimeler

• Brotizolam
• Ghrelin
• Appetite

Brotizolamla İndüklenen İştahın Farelerde Ghrelin Seviyeleri Üzerine Etkisi

Öz

Amaç: Evcil hayvanlarda iştah, metabolik profil, enfeksiyon ve beslenme şeklinden etkilenmektedir. İştahın düzenlenmesi pek çok karmaşık fizyolojik süreci içerir. Ghrelin en iyi bilinen iştah arttırıcı hormondur. İştah da lateral hipotalamus tarafından yönetilir. Öte yandan, ventromedial çekirdek çoğunlukla tokluk ile ilişkilidir. 1,4-benzodiazepin türevinin bir üyesi olan brotizolam, ventromedial hipotalamusun aktivitesini bloke eder ve evcil hayvanlarda iştahı artırmak için kullanılır. Bu çalışmanın amacı, brotizolamın iştah artırıcı hormon olan serum ghrelin düzeylerine etkisini araştırmaktır.Yöntem: Toplam 16 fare, kontrol (%0,9 NaCl, IP) ve brotizolam (2 μg/kg, IP) olarak 2 eşit gruba ayrıldı. Her iki grupta da uygulamadan 30 dakika sonra kan örnekleri toplandı. Serum ghrelin seviyeleri, Enzyme-Linked Immunoassay ile belirlendi.Bulgular: Kontrol ve brotizolam grupları arasında anlamlı bir fark vardı (P<0.001). Çalışmanın sonuçları, brotizolam uygulamasının serum ghrelin düzeylerini artırdığını gösterdi.Sonuç: Ventromedial hipotalamus ve tokluğu baskılayan brotizolam, lateral hipotalamusun aktivasyonu ve ghrelin düzeylerinin artması ile iştahı aktive edebili. Ayrıca ghrelin iştahın endokrin belirteci olarak kullanılabilir.

Anahtar Kelimeler

• Brotizolam
• Ghrelin
• İştah
• İştah

Kaynakça

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