Kolon adenokarsinomlarında ve adenomatöz poliplerinde Beta-Catenin ve Sox2 ekspresyonu ve klinikopatolojik parametreler ile ilişkileri

Öz

Amaç: β-katenin ve Sox-2’nin kolonun adenomatöz polipleri ve adenokarsinomunda immünohistokimyasal ekspresyonunun araştırılması, adenom-karsinom sekansında bu belirteçlerin yeri ve etkisinin değerlendirilmesidir. Yöntem: 56 düşük dereceli displazi içeren tübüler adenom(DDDTA), 53 yüksek dereceli displazi içeren tübüler adenom (YDDTA), 44 tübülovillöz adenom (TVA), 29 villöz adenom (VA) ve 60 kolon adenokarsinomu çalışma kapsamına alındı. β-katenin için nükleer ve sitoplazmik, Sox-2 için nükleer boyanma değerlendirildi ve semikantitatif skorlama yapıldı. Bulguların gruplar arasında ve klinikopatolojik parametrelerle ilişkisi değerlendirildi. Bulgular: Nükleer β-katenin ekspresyonu adenokarsinomda poliplere nazaran anlamlı olarak fazla saptandı. Poliplerde ise VA ile YDDTA arasında VA lehine; TVA ile YDDTA ve TVA ile DDDTA grupları arasında TVA lehine farklıdır. Sitoplazmik β-katenin, adenokarsinom ile VA, TVA, YDDTA ve DDDTA grupları arasında adenokarsinom lehine; VA ile TV, YDDTA ve DDDTA grupları arasında VA lehine; TVA ile YDDTA grupları arasında TVA lehine ve YDDTA ile DDDTA arasında YDDTA lehine istatistiksel olarak anlamlı farklılık bulunmuştur. Sonuç: Gruplardaki β-katenin sonuçları literatür ile uyumludur. Sox2 ekspresyonu malign potansiyeli destekler niteliktedir. Adenom-karsinom sekansında Sox2’nin etkisinin ayrıntılı açıklanabilmesi için moleküler çalışmalar ile desteklenen geniş serili araştırmalara gereksinim vardır.

Anahtar Kelimeler

• kolon
• adenomatöz polip
• adenokarsinom
• beta katenin
• sox2

The expression of Beta-Catenin and Sox2 in adenocarcinoma and adenomatous polyps of the colon and their association with clinicopathological parameters

Öz

Objective: Our aim was to investigate the immunohistochemical expression of β-catenin and Sox-2 in adenomatous polyps and adenocarcinoma of colon and also to evaluate the effects of these markers in adenoma-carcinoma sequence and their association with clinicopathological parameters. Methods: Fifty-six tubular adenomas with low grade dysplasia (TALGD), 53 tubular adenomas with high grade dysplasia (TAHGD), 44 tubulovillous adenomas (TVA), 29 villous adenomas (VA) and 60 adenocarcinomas were included in the study. The nuclear staining of Sox2 was evaluated as well as both nuclear and cytoplasmic stainings of β-catenin. A semiquantitative scoring was performed. The results were compared between the groups and the relationship of the results with clinicopathological parameters was evaluated. Results: Nuclear and cytoplasmic β-catenin expressions of the adenocarcinomas were higher than polyps. The expressions in the VA and TVA polyp groups were higher than the expressions in TAHGD and TALGD, respectively. Membranous β-catenin expression in the adenocarcinoma was higher than the polyps except VA. The evaluation between polyp groups with respect to membranous β-catenin staining revealed a statistically significantly difference in favor of VA compared with TVA, TAHGD and TALGD; in favor of TAHGD compared with TVA, in favor of TVA compared with TALGD while it was found statistically significantly higher in TAHGD than TALGD. Conclusion: The results regarding β-catenin expression of the polyp groups were consistent with the literature. There was a positive correlation between β-catenin expression (nuclear and cytoplasmic) and malignancy. High Sox2 expressions were found correlated with malignancy potential. Large sampling size investigations to be supported by further molecular studies are needed to clarify the effect of Sox2 expression in the sequence of adenoma-carcinoma comprehensively.

Anahtar Kelimeler

• colon
• adenomatous polyp
• adenocarcinoma
• beta catenin

Kaynakça

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