Antikolinerjik ilaçlardan Biperiden civciv embriyolarında erken dönemde nöral tüp gelişimini etkiler mi?

Öz

Amaç: Biperiden, hem merkezi hem de periferik olarak etki gösteren antikolinerjik bir ajandır. Klinik uygulamada hem nöroleptik tedavinin ekstrapiramidal yan etkilerine hem de Parkinson hastalığının semptomlarına karşı kullanılır. Mevcut çalışmanın amacı farklı dozlarda Biperiden uygulamasının 48 saatlik civciv embriyolarında nöral tüp kapanması üzerindeki potansiyel toksik etkisini belirlemektir. Yöntem: Çalışmada 60 adet döllenmiş yumurta kullanıldı. Tüm yumurtalar kuluçka makinesine yerleştirildi ve her grupta 15 yumurta olacak şekilde dört gruba ayrıldı: Kontrol, BPD1, BPD2 ve BPD3. Yirmi sekiz saatlik inkübasyonda, Biperiden gruplarına subblastodermik olarak üç farklı Biperiden dozu uygulandı. 48 saatlik inkübasyonun sonunda tüm yumurtalar açılarak embriyolar diseke edildi. Embriyolar morfolojik ve histopatolojik olarak değerlendirildi. Bulgular: Mevcut çalışmanın sonuçlarına göre ortalama baş-popo uzunluğu ve somit sayısı dozla orantılı olarak azalma eğilimindeydi. Doz arttıkça deney gruplarında açık nöral tüp ve gelişmemiş embriyo sayısı istatistiksel olarak anlamlı şekilde artmıştır. Somit sayısına göre değerlendirilen embriyoların Hamburger-Hamilton evreleri açısından da gruplar arasında anlamlı fark vardı. Kontrol, BPD1 ve BPD2 gruplarındaki embriyolar evre 13’te, BPD3 grubundakiler ise evre 12’de gözlendi. Sonuç: Çalışmamızda erken civciv embriyolarında Biperiden’in düşük dozda bile nöral tüp kapanmasında teratojeniteye neden olduğunu gösterilmiştir. Doza bağlı olarak somit sayısı ve baş-popo uzunluğu azalmış, yüksek dozda ise gelişim geriliğine neden olmuştur.

Anahtar Kelimeler

• Biperiden
• Civciv Embriyosu
• Nöral tüp

Does the anticholinergic drug Biperiden affect early neural tube development in chick embryos?

Öz

Objective: Biperiden (BPD) is an anticholinergic agent that acts both centrally and peripherally. It is used to counteract both extrapyramidal side effects of neuroleptic treatment and symptoms of Parkinson’s disease in clinical practice. Current study was layout to determine the potential toxic effect of different doses of Biperiden on neural tube closure in 48hr chick embryos. Method: Sixty fertilized eggs were used in the study. All eggs were placed in the incubator and divided into four groups (15 eggs in each); Control, BPD1, BPD2 and BPD3. At 28hr of incubation, three different doses of Biperiden were administered subblastodermically in all BPD groups. At the end of 48hr of incubation, all eggs were opened and embryos were dissected and evaluated morphologically and histopathologically. Results: According to these results, the mean crown-rump length and somite number tended to decrease proportionally with the dose. As the dose increases, the number of open neural tube and undeveloped embryos in the experimental groups also increases. There was also a significant difference between the groups in terms of Hamburger-Hamilton stages of embryos evaluated according to the number of somite. Embryos in the Control, BPD1 and BPD2 groups were observed at stage 13, and those in the BPD3 group were observed at stage 12. Conclusion: These results showed that Biperiden even in the low dose has teratogenicity on neural tube closure in early chick embryos. The somite numbers and crown-rump length were decreased depending on the dose and Biperiden caused developmental retardation in high doses.

Anahtar Kelimeler

• Biperiden
• Chick Embryo
• Neural Tube

Kaynakça

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