DNA Protective Assay and Some Biochemical Properties of Galium Species

Yıl 2024, , 24 - 30, 01.07.2024

Yener Tekeli , Ömer Kılıç , Elif Esra Altuner

https://doi.org/10.18586/msufbd.1451599

Öz

Herbal-derived drugs prepared using various extracts obtained from different organs of plants that have been scientifically proven to be “medicinal” or directly from these plants are often used today as a method to prevent or treat diseases in humans and animals. This method, called phytotherapy, is a rational, evidence-based, and allopathic treatment method and deals with which active substance group is responsible for the biological effects. In this context, the phytotherapeutic effectiveness of Galium species, which are also used for medicinal purposes among the public, has been examined and proven in many studies. In this study, the biochemical efficacies of five different Galium species were measured and their antioxidant, antimicrobial, and DNA protective effects were tested. It was found that the tested Galium species showed remarkable biochemical efficacies. The results were also compared with the results of some other studies in the literature.

Anahtar Kelimeler

Antimicrobial, Antioxidant, DNA, Phytotherapy, Protective assay

DNA Koruyucu Testi ve Galium Türlerinin Bazı Biyokimyasal Özellikleri

Öz

Bilimsel olarak “tıbbi” olduğu kanıtlanmış bitkilerin farklı organlarından elde edilen çeşitli ekstraktlar veya doğrudan bu bitkilerden elde edilen çeşitli ekstraktlar kullanılarak hazırlanan bitkisel kökenli ilaçlar, günümüzde insanlarda ve hayvanlarda hastalıkların önlenmesi veya tedavisinde bir yöntem olarak sıklıkla kullanılmaktadır. Fitoterapi adı verilen bu yöntem akılcı, kanıta dayalı, allopatik bir tedavi yöntemi olup, biyolojik etkilerden hangi etken madde grubunun sorumlu olduğunu ele almaktadır. Bu bağlamda halk arasında tıbbi amaçlı da kullanılan Galium türlerinin fitoterapötik etkinliği birçok çalışmada incelenmiş ve kanıtlanmıştır. Bu çalışmada beş farklı Galium türünün biyokimyasal etkinlikleri ölçülmüş, antioksidan, antimikrobiyal ve DNA koruyucu etkileri test edilmiştir. Test edilen Galium türlerinin dikkate değer biyokimyasal etkinlik gösterdiği tespit edildi. Sonuçlar ayrıca literatürdeki diğer bazı çalışmaların sonuçlarıyla da karşılaştırıldı.

Anahtar Kelimeler

Antimikrobiyal, Antioksidan, DNA, Galium türleri, Fitoterapi, Koruyucu tahlil

Kaynakça

• [1] Sevindik, M., Akgul, H., Pehlivan, M., & Selamoglu, Z. (2017). Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull, 26(7), 4757-4763.
• [2] Turan A., Celik I. Antioxidant and hepatoprotective properties of dried fig against oxidative stress and hepatotoxicity in rats. Int J Biol Macromol. 2016, 91:554–9.
• [3] Sharma A., Bajaj P., Bhandari A., Kaur G. From ayurvedic folk medicine to preclinical neurotherapeutic role of a miraculous herb, Tinospora cordifolia. Neurochem Int 2020, 141:104891.
• [4] Akgül, H., Mohammed, F. S., Kına, E., Uysal, İ., Sevindik, M., & Doğan, M. (2022). Total Antioxidant and Oxidant Status and DPPH Free radical activity of Euphorbia eriophora. Turkish Journal of Agriculture-Food Science and Technology, 10(2), 272-275.
• [5] Pehlivan, M., Mohammed, F. S., Sevindik, M., & Akgul, H. (2018). Antioxidant and oxidant potential of Rosa canina. Eurasian Journal of Forest Science, 6(4), 22-25.
• [6] Poprac, P., Jomova, K., Simunkova M., Kollar V., Rhodes, C.J., Valko, M. Targeting Free Radicals in Oxidative Stress-Related Human Diseases, Trends Pharmacol Sci 2017, 38:7, 592–607.
• [7] Gonzales M., Villena G.K., Kitazono A.A. Evaluation of the antioxidant activities of aqueous extracts from seven wild plants from the Andes using an in vivo yeast assay. Results Chem 2020, 3:100098.
• [8] Pehlivan, M., Mohammed, F. S., Şabik, A. E., Kına, E., Dogan, M., Yumrutaş, Ö., & Sevindik, M. (2021). Some Biological activities of ethanol extract of Marrubium globosum. Turkish Journal of Agriculture-Food Science and Technology, 9(6), 1129-1132
• [9] Uysal, İ., Mohammed, F. S., Şabik, A. E., Kına, E., & Sevindik, M. (2021). Antioxidant and Oxidant status of medicinal plant Echium italicum collected from different regions. Turkish Journal of Agriculture-Food Science and Technology, 9(10), 1902-1904.
• [10] Senio S., Pereira C., Vaz J., Sokovic M., Barros L., Ferreira I.C.F.R Dehydration process influences the phenolic profile, antioxidant and antimicrobial properties of Galium aparine L. Ind Crops Prod 2021, 120, 97–103.
• [11] Albassam A.A., Alanazi A., Alhaqbani N., Alhoti F., Almalki Z.S., Alshehri A.M., Alzahrani, J. The potential of drug-herbal interaction among patients with chronic diseases in Saudi Arabia. Complement Ther Clin Pract 2021, 43, 2–5.
• [12] Elateek S.Y., Salem L.M., Ahmed E.S., Khalil W.K.B S.aureus isolates from hospital clinics induce ROS-mediated DNA damage, apoptosis and gene expression alterations in male mice. Gene Rep. 2021, 23, 1–8.
• [13] Tang H., Hosein A., Mattioli-Belmonte M) Traditional Chinese Medicine and orthopedic biomaterials: Host of opportunities from herbal extracts. Mater Sci Eng C. 2021, 120, 111760.
• [14] Truong Hoang Q., Lee D.Y., Choi D.G., Kim, Y.C., Shim M.S. Efficient and selective cancer therapy using pro-oxidant drug-loaded reactive oxygen species (ROS)-responsive polypeptide micelles. J Ind Eng Chem. 2021, 95, 101–8.
• [15] Akdeniz S., Pollen Morphology Of The Some Taxa Belongıng To Genus Asperula L., Galıum L. (Rubıaceae) Dıstrıbuted In Turkey, Bitlis Eren University Graduate School of Natural and Applied Sciences Department of Biology 2019, 1-38.
• [16] Karou S.D., Tchacondo T., Djikpo Tchibozo, M.A., Abdoul-Rahaman S., Anani K., Koudouvo K, De Souza ,C. Ethnobotanical study of medicinal plants used in the management of diabetes mellitus and hypertension in the Central Region of Togo, Pharm Biol 2011, 49(12), 1286–97.
• [17] Calis I., Ergun F., Tanker M. Iroidler Iridoid Hetero zitleri. J Pharm Sci.2021, 10, 31–47.
• [18] Ergun F., Deliorman D., Velioglu A., Sener B., Bozok Johansson C., Antimicrobial activities of Galium species. J Pharm Gazi 1999, 16(1), 7–11.
• [19] Korkmaz, N., Dayangaç, A., & Sevindik, M. (2021). Antioxidant, antimicrobial and antiproliferative activities of Galium aparine. Journal of Faculty of Pharmacy of Ankara University, 45(3), 554-564.
• [20] Abousheenah K.M.F.B.A Investigation of Biological Activities of Galium incanum Plant Extracts, Kastamonu University Graduate School of Natural and Applied Sciences 2019.
• [21] Acemi R.K, Acemi A., Cakir M., Polat E.G., Ozen F., Preliminary screening the antioxidant potential of in vitro-propagated Amsonia orientalis: An example to sustainable use of rare medicinal plants in pharmaceutical studies, Sustain Chem Pharm. 2020, 1, 100302.
• [22] Mwamatope, B., Tembo, D., Chikowe, I., Kampira, E., Nyirenda, C. Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants. Sci Afr 2020, 9:e00481.
• [23] Paiva L., Lima E., Motta M, Marcone M., Baptista J. , Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis. Curr Res Food Sci. 2020, 3, 227–34.
• [24] Ahmed W, Azmat R, Mehmood A, Qayyum A, Ahmed R, Khan, S.U., Liaquat, M., Naz, S., Ahmad, S. The analysis of new higher operative bioactive compounds and chemical functional group from herbal plants through UF-HPLC-DAD and Fourier transform infrared spectroscopy methods and their biological activity with antioxidant potential process as future, Arab J Chem 2021, 14(2), 1–12.
• [25] Okan O.T., Varlıbaş H., Öz M., Deniz İ. Antioksidan Analiz Yöntemleri ve Doğu Karadeniz Bölgesinde Antioksidan Kaynağı Olarak Kullanılabilecek Odun Dışı Bazı Bitkisel Ürünler. Kastamonu Üni., Orman Fakültesi Dergisi, 2013, 13 (1): 48-59.
• [26] Buyuktuncel E., Toplam fenolik içerik ve antioksidan kapasite tayininde kullanilan baslica spektrofotometrik yöntemler, Marmara Pharm J. 2013, 2(17), 93–103.
• [27] Chen LY, Cheng C.W, Liang J.Y, Effect of esterification condensation on the Folin–Ciocalteu method for the quantitative measurement of total phenols, Food Chem 2015, 170,10–5.
• [28] Brand-Williams W., Cuvelier M.E., Berset C, Use of a free radical method to evaluate antioxidant activity, LWT - Food Science and Technology, 28, 25–30.
• [29] Hamlaoui I., Bencheraiet R., Bensegueni R., Bencharif M. Experimental and theoretical study on DPPH radical scavenging mechanism of some chalcone quinoline derivatives. J Mol Struct. 1995, 15:1156, 385–9.
• [30] Bokhari J., Khan M.R., Shabbir M., Rashid U., Jan S., Zai J.A. Evaluation of diverse antioxidant activities of Galium aparine, Spectrochim Acta - Part Mol Biomol Spectrosc. 2013, 102:24–9.
• [31] Akar Z., Kucuk M., Dogan H. A. A new colorimetric DPPH• scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs, J Enzyme Inhib Med Chem, 2020, 32:1, 640–7.
• [32] Faleh Al-blewi, F., Almehmadi M.A., Reda Aouad M., Bardaweel S.K., Sahu P.K., Messali M., Nezki R.,El-Ashry, E.S.H. Design, synthesis, ADME prediction and pharmacological evaluation of novel benzimidazole-1,2,3-triazole-sulfonamide hybrids as antimicrobial and antiproliferative agents, Chem Cent J. 2018,, 12:110.
• [33] Verma K, Shrivastava D, Kumar G. Antioxidant activity and DNA damage inhibition in vitro by a methanolic extract of Carissa carandas (Apocynaceae) leaves. J Taibah Univ Sci. 2015 Jan;9(1):34–40.
• [34] Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Vol. 28, LWT - Food Science and Technology. Academic Press; 1995. p. 25–30.
• [35] Trkan K, Kasim T, Bircan eken, Murat K. DNA damage protecting activity and in vitro antioxidant potential of the methanol extract of Cherry (Prunus avium L). J Med Plants Res (Internet). 20148(19):715–26. 0
• [36] Kaur, P., Purewal, S.S., Sandhu, K.S., Kaur, M. DNA damage protection: an excellent application of bioactive compounds. Bioresour Bioprocess 2019, 6:1, 2. doi:10.1186/s40643-019-0237-9
• [37] Anand, T., Mahadeva, N., Phani, K.G., Farhath, K., Antioxidant and DNA Damage Preventive Properties of Centella asiatica (L) 2010, 2:17, 53–8.
• [38] Yang, J.I., Yeh, C.C., Lee, J.C., Yi, S.C., Huang, H.W., Tseng, C.N., Chang, H.W., Aqueous Extracts of the Edible Gracilaria tenuistipitata are Protective Against H2O2-Induced DNA Damage, Growth Inhibition, and Cell Cycle Arrest. Molecules 2012, 17:6, 7241–54.
• [39] Adinortey M., Ansah C., Weremfo A., Adinortey, C., Adukpo G., Ameyaw E., Nyarko A.W., DNA damage protecting activity and antioxidant potential of Launaea taraxacifolia leaves extract, J Nat Sci Biol Med 2018, 9:1, 6.
• [40] Berk S., Tepe B., Arslan S., Sarikurkcu C, , Screening of the antioxidant, antimicrobial and DNA damage protection potentials of the aqueous extract of Asplenium ceterach DC, Ajol 2011, 10:44, 8902–8.