Postbiotic metabolites of probiotic Lactiplantibacillus plantarum in natural yoghurts from Muş region and their effects on colon cancer cell lines

Öz

Probiotic Lactic Acid Bacteria (LAB) can exhibit different characteristics depending on their source of isolation, and they may also possess varying potentials in terms of postbiotic metabolite amounts and anticancer activity. Therefore, 22 of 125 LAB isolates isolated from yogurt were determined to be like Lactiplantibacillus plantarum using MALDI-TOF MS. When the probiotic resistance of L. plantarum isolates to different pH, pepsin, pancreatin, and bile salts was investigated, all isolates were found to show good activity. The amount of postbiotic metabolites of six L. plantarum strains (3, 7, 13, 25, 26, and 29) and L. plantarum STD, which showed good probiotic properties, was determined by HPLC. Tartaric acid, pyruvic acid, and malic acid synthesis were not detected in these isolates. It was determined that these isolates synthesized the most acetic acid and the least succinic acid. Furthermore, it was observed that these isolates exhibited improved anticancer activity on colon cancer cell lines (HT-29 and Caco-2), depending on increasing concentrations. It is believed that L. plantarum isolates will contribute to the food and health industries with further support from new in vivo studies.

Anahtar Kelimeler

• Anticancer
• LAB
• Lactiplantibacillus plantarum
• Postbiotic metabolite
• Probiotic

Proje Numarası

BAP-21-FEF-4901-03

Muş yöresinin doğal yoğurtlarındaki probiyotik Lactiplantibacillus plantarum’ların postbiyotik metabolitleri ve kolon kanser hücre hattı üzerindeki etkileri

Öz

Probiyotik Laktik Asit Bakterileri (LAB) izole edildiği kaynağa bağlı olarak farklı karakterde olabildikleri gibi, postbiyotik metabolit miktarı ve antikanser aktivite bakımından farklı potansiyellere sahip olabilirler. Bu nedenle yoğurttan izole edilmiş 125 LAB izolatının MALDI-TOF MS ile 22 izolatın Lactiplantibacillus plantarum’a benzerlik gösterdikleri belirlendi. L. plantarum izolatları farklı pH, pepsin, pankreatin ve safra tuzuna karşı probiyotik dirençleri araştırıldığında, bütün izolatların iyi aktivite gösterdiği belirlendi. İyi probiyotik özellik gösteren altı L. plantarum (3, 7, 13, 25, 26 ve 29) suşu ile L. plantarum STD’in postbiyotik metabolit miktarı HPLC ile belirlendi. Bu izolatların tartarik asit, pürivik asit ve malik asit sentezi tespit edilmedi. Bu izolatların en fazla asetik acid, en az süksinik acid sentezledikleri belirlendi. Ayrıca bu izolatların kolon kanseri hücre hatları (HT-29 ve Caco-2) üzerinde konsantrasyon artışına bağlı olarak daha iyi antikanser aktivite sergiledikleri gözlemlendi. L. plantarum izolatlarının yeni in vivo çalışmalar ile desteklenerek gıda ve sağlık endüstrisine katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

• Antikanser
• LAB
• Lactiplantibacillus plantarum
• Postbiyotik metabolit
• Probiyotik

Proje Numarası

BAP-21-FEF-4901-03

Kaynakça

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