Araştırma Makalesi
BibTex RIS Kaynak Göster

KARAYEMİŞ MEYVESİNDEN (Laurocerasus officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ

Yıl 2014, Cilt: 2 Sayı: 2, 280 - 288, 01.12.2014

Fikret Türkan Neslihan Balcı Halis Şakiroğlu

Öz

Bu çalışmada karayemiş (Laurucerasus officinalis Roem.)
meyvesinden Glutatyon S- Transferaz (EC 2.5.1.18) enzimi
saflaştırıldı ve karakterize edildi. Saflaştırma işlemi tek basamakta afinite
kromatografisiyle yapıldı. GST enziminin saflığım kontrol etmek için SDS-PAGE
elektroforezi uygulandı. Enzim ile ilgili
kinetik çalışmalar olarak optimum pH, optimum sıcaklık, optimum iyonik şiddet
ile indirgenmiş glutatyon (GSH) ve 1- kloro 2,4- dinitrobenzen (CDNB) için KM
ve V^ çalışmaları yapıldı. Çalışma sonuçlarına göre GSH için KMve V^
değerleri 0.194 mM ve 0.038 EU/ml, CDNB için
KMve V^ değerleri 0.353 mM ve 0.099 EU/ml olarak bulundu. Enzim
aktivitesi üzerine bazı pestisitlerin etkisi araştırıldı. Bu
pestisitlerden; diklorvos, cypermethrin, imidacloprid, fenoxaprop-p-ethyl,
glyphosate isopropilamin tuzunun inhibitör özelliği gösterdiği; haloxyfop-p-methyl, lambda- cyhalothrin ve 2,4-
dichlorophenoxyasetic asit dimethylamine tuzunun ise aktivatör olarak
davrandığı tespit edildi.




Anahtar Kelimeler

Glutatyon S-Transferaz Glutatyon Inhibisyon Saflaştırma

Kaynakça

  • [1]. Var, M. Seed Fatty Acıd Composıtıon In Wıld Form And Cultıvars Of Laurocerasos Offıcınalıs Roem. Pakistan Journal of Botany, 27(2), 305-308, 1995. [2]. Baytop, T., Türkiye'de Zehirli Bitkiler, Bitki Zehirlenmeleri ve Tedavi Yöntemleri; Yayın 3 560; Istanbul Üniversitesi Yayinlan: Istanbul, Turkey, 1989. [3]. Milan, S.P.A. The Macdonald Encyclopedia of Medicinal Plants; Macdonald: London, U.K. p 252, 1984. [4]. Ayaz, F.A., Studies on Water Soluble Sugar and Sugar Alcohol in Cultivar and Wild Forms of Laurocerasus Offlcinalis Roem, Pakistan J. Bot., 29(2), 331-336, 1997. [5]. Robards, K., Prenzlere, P.D., Tucker, G., Swatsitang, P., Glover, W., Phenolic Compounds and Their Role in Oxidative Processes in Fruits, Food Chem., 66, 401-436, 1999. [6]. Vaya, J., Belinky, P.A., Aviram, M., AntioxidantCconstituents from Licorice Roots: Isolation, Structure Elucidation and Antioxidative Capacity Toward LDL Oxidation, Free Radical Biol. Med., 23 (2), 302-313, 1997. [7]. Vinson, J.A., Su, X., Zubik L., Bose, P., Phenol Antioxidant Ouality in Food: Fruits, J Agric. Food Chem., 49, 5315-5321, 2001. [8]. Smirnoff, N., The Function andMetabolism of Ascorbic Acid in Plants. Ann. Bot. London, 78, 661-669, 1996. [9]. Storz, G., Imlay, J., Oxidative Stress, Curr. Opin. Microbiol. 2, 188-194, 1999. [10]. Ulubelen, A., Topçu, G., Tan, N., Ölçal, S., Johanson, C, Üçer, M., Birman, H., Tamer, S., Biological Activities of a Turkish Medicinal Plant, Prangos Platychlaena, J. Ethnopharmacol., 45(3), 193-197, 1995. [11]. Yan, X., Murphy, B.T, Hammond, G.B., Vinson, J.A., Neto, C.C., Antioxidant Activities and Antitumor Screening of Extracts from Cranberry Fruit (Vaccinium Macrocarpon), J. Agric. Food Chem., 50, 5844-5846, 2002. [12]. Ranson, H., Rossiter, L., Ortelli, F, Jensen, B., Wang, X.L., Roth, C.W, Collins, F.H., Hemingway, J., Identification of a Novel Class of Insect Glutathione S-Transferases Involved in Resistance to ddt in the Malaria Vector Anopheles gambiae, Biochem. 359, 295-304, 2001. [13]. Lumjuan, N., Rajatıleka, S., Changsom, D., Wıcheer, J., Leelapat, P, Prapanthadara, L., Somboon, P, Lycett, G., and Ranson, H., The Role of the Aedes Ring Resistance to DDT and Pyrethroid Insectidies, Insect. Biochem. Mol. Biol, 41, 203-209, 2011. [14]. Furtado, A.F., Magalhaes, T, Macoris, M.L.G., and Righetti, M.T M., Ayres, C.F.J., Resistance to the Organophosphate Temephos: Mechanisms, Evolution and Reversion in an Aedes Aegypti Laboratory Strain from Brazil. Acta. Trop. 113, 180-189, 2010. [15]. Low, W.Y, S.C., Ng, H.L., Gorman, M.A., Morton, C J., Pyke, J., Mecconville, M.J., Bieri, M., Mok, Y.F., Robin, C, Gooley, P R., Parker, M.W., and Batterham, P, Recognotion and Detoxifıcation of the Insecticide DDT by Drosophila Melanogaster Glutathione S-Transferase, J. Mol. Biol, 399, 358-366, 2010. [16]. Clark, A.G., The Glutathione S-trasferases and Resistance to Insecticides, pp. 369-378 InJ. D. Hayes, CB.,PickettandT.J.,Mantle [eds.]. Glutathione S-transferases and Drug Resistance. Proc. 3rd Int. GST Conferance, Royal College of Physicians, Edinburgh, Scotland, UK, 20-30 Aug 1989. [17]. Fukami, J.I., Shishido, T. Nature of a Soluble Glutathione-Dependent Enzyme System Active in Cleavage of Methyl Parathion to Desmethyl Parathion, J. Econ. Entomol, 59, 1338-1346, 1996. [18]. Edwards R, Dixon D.P, Walbot, V, Plant Glutathione S-transferases: Enzymes with Multiple Functions in Sickness and in Health, Trends Plant Sci., 5, 193-198, 2000. [19]. Dixon, D. P., Davis, B. G., Edwards, R. Functional divergence in the glutathione transferase superfamily in plants identification of two classes with putative functions in redox homeostasis in Arabidopsis thaliana. Journal of Biological Chemistry, 277(34), 30859-30869, 2002. [20]. Banerjee, S., Goswami, R. GST profile expression study in some selected plants: in silico approach. Molecular and cellular biochemistry, 336(1-2), 109-126, 2010. [21]. Soranzo, N., Gorla, M. S., Mizzi, L., De Toma, G., Frova, C. Organisation and structural evolution of the rice glutathione S-transferase gene family. Molecular genetics and genomics, 271(5), 511-521, 2004.
  • [22]. Erat, M., Guvercin, S., Sakiroglu, H. Determination of some kinetic and characteristic properties of glutathione S-transferase from bovine erythrocytes. Protein and peptide letters, 15(1), 6-12, 2008.
  • [23]. Akkemik, E., Taser, R, Bayindir, A., Budak, H., Ciftci, M., Environmental Toxicology an Pharmacology, 34, 888-894, 2012.
  • [24]. Chun-hua, Z., Ze-ying, W., Ting, J., Ying, G. Rice Science, 20(3), 173-178, 2013.
  • [25]. Toribio, F., Martinez-Lara, E., Pascual, P., Lopez-Barea, J. Methods for purification of glutathione peroxidase and related enzymes. Journal of Chromatography B: Biomedical Sciences and Applications, 684(1), 77-97, 1996.
  • [26]. Simons, P.C., Vander Jagt, D.L. Methods Enzymol, 11 ,235-241, 1981. [27]. Habig, W.H., Pabst, M.J., Jakoby, W.B., Glutathione S-transferases. The first enzymmatic step in mercapturic acid formation. J Biol Chem, 246, 7130-7139, 1974.
  • [28]. Bradford M.A. Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal Biochem., 72, 248-254, 1976.
  • [29]. Awasthi, Y.C, Singh, S.V., Purification and Characterization of a New form of Glutathione S-Transferase from Human-Erythrocytes. Biochem. Biophys. Res. Commun, 125, 1053-1060, 1984.
  • [30]. Demirdag, R., Yerlikaya, E., Kufrevioglu, O.I., Gundogdu C, Purification of Glutathione S-transferase Isoenzymes from Tumour and Nontumour Human Stomach and Inhibitory Effects of Some Heavy Metals on Enzymes Activities, Journal of Enzyme Inhibition and Medicinal Chemistry, 28(5), 911-915, 2013.

Yıl 2014, Cilt: 2 Sayı: 2, 280 - 288, 01.12.2014

Fikret Türkan Neslihan Balcı Halis Şakiroğlu

Öz

Kaynakça

  • [1]. Var, M. Seed Fatty Acıd Composıtıon In Wıld Form And Cultıvars Of Laurocerasos Offıcınalıs Roem. Pakistan Journal of Botany, 27(2), 305-308, 1995. [2]. Baytop, T., Türkiye'de Zehirli Bitkiler, Bitki Zehirlenmeleri ve Tedavi Yöntemleri; Yayın 3 560; Istanbul Üniversitesi Yayinlan: Istanbul, Turkey, 1989. [3]. Milan, S.P.A. The Macdonald Encyclopedia of Medicinal Plants; Macdonald: London, U.K. p 252, 1984. [4]. Ayaz, F.A., Studies on Water Soluble Sugar and Sugar Alcohol in Cultivar and Wild Forms of Laurocerasus Offlcinalis Roem, Pakistan J. Bot., 29(2), 331-336, 1997. [5]. Robards, K., Prenzlere, P.D., Tucker, G., Swatsitang, P., Glover, W., Phenolic Compounds and Their Role in Oxidative Processes in Fruits, Food Chem., 66, 401-436, 1999. [6]. Vaya, J., Belinky, P.A., Aviram, M., AntioxidantCconstituents from Licorice Roots: Isolation, Structure Elucidation and Antioxidative Capacity Toward LDL Oxidation, Free Radical Biol. Med., 23 (2), 302-313, 1997. [7]. Vinson, J.A., Su, X., Zubik L., Bose, P., Phenol Antioxidant Ouality in Food: Fruits, J Agric. Food Chem., 49, 5315-5321, 2001. [8]. Smirnoff, N., The Function andMetabolism of Ascorbic Acid in Plants. Ann. Bot. London, 78, 661-669, 1996. [9]. Storz, G., Imlay, J., Oxidative Stress, Curr. Opin. Microbiol. 2, 188-194, 1999. [10]. Ulubelen, A., Topçu, G., Tan, N., Ölçal, S., Johanson, C, Üçer, M., Birman, H., Tamer, S., Biological Activities of a Turkish Medicinal Plant, Prangos Platychlaena, J. Ethnopharmacol., 45(3), 193-197, 1995. [11]. Yan, X., Murphy, B.T, Hammond, G.B., Vinson, J.A., Neto, C.C., Antioxidant Activities and Antitumor Screening of Extracts from Cranberry Fruit (Vaccinium Macrocarpon), J. Agric. Food Chem., 50, 5844-5846, 2002. [12]. Ranson, H., Rossiter, L., Ortelli, F, Jensen, B., Wang, X.L., Roth, C.W, Collins, F.H., Hemingway, J., Identification of a Novel Class of Insect Glutathione S-Transferases Involved in Resistance to ddt in the Malaria Vector Anopheles gambiae, Biochem. 359, 295-304, 2001. [13]. Lumjuan, N., Rajatıleka, S., Changsom, D., Wıcheer, J., Leelapat, P, Prapanthadara, L., Somboon, P, Lycett, G., and Ranson, H., The Role of the Aedes Ring Resistance to DDT and Pyrethroid Insectidies, Insect. Biochem. Mol. Biol, 41, 203-209, 2011. [14]. Furtado, A.F., Magalhaes, T, Macoris, M.L.G., and Righetti, M.T M., Ayres, C.F.J., Resistance to the Organophosphate Temephos: Mechanisms, Evolution and Reversion in an Aedes Aegypti Laboratory Strain from Brazil. Acta. Trop. 113, 180-189, 2010. [15]. Low, W.Y, S.C., Ng, H.L., Gorman, M.A., Morton, C J., Pyke, J., Mecconville, M.J., Bieri, M., Mok, Y.F., Robin, C, Gooley, P R., Parker, M.W., and Batterham, P, Recognotion and Detoxifıcation of the Insecticide DDT by Drosophila Melanogaster Glutathione S-Transferase, J. Mol. Biol, 399, 358-366, 2010. [16]. Clark, A.G., The Glutathione S-trasferases and Resistance to Insecticides, pp. 369-378 InJ. D. Hayes, CB.,PickettandT.J.,Mantle [eds.]. Glutathione S-transferases and Drug Resistance. Proc. 3rd Int. GST Conferance, Royal College of Physicians, Edinburgh, Scotland, UK, 20-30 Aug 1989. [17]. Fukami, J.I., Shishido, T. Nature of a Soluble Glutathione-Dependent Enzyme System Active in Cleavage of Methyl Parathion to Desmethyl Parathion, J. Econ. Entomol, 59, 1338-1346, 1996. [18]. Edwards R, Dixon D.P, Walbot, V, Plant Glutathione S-transferases: Enzymes with Multiple Functions in Sickness and in Health, Trends Plant Sci., 5, 193-198, 2000. [19]. Dixon, D. P., Davis, B. G., Edwards, R. Functional divergence in the glutathione transferase superfamily in plants identification of two classes with putative functions in redox homeostasis in Arabidopsis thaliana. Journal of Biological Chemistry, 277(34), 30859-30869, 2002. [20]. Banerjee, S., Goswami, R. GST profile expression study in some selected plants: in silico approach. Molecular and cellular biochemistry, 336(1-2), 109-126, 2010. [21]. Soranzo, N., Gorla, M. S., Mizzi, L., De Toma, G., Frova, C. Organisation and structural evolution of the rice glutathione S-transferase gene family. Molecular genetics and genomics, 271(5), 511-521, 2004.
  • [22]. Erat, M., Guvercin, S., Sakiroglu, H. Determination of some kinetic and characteristic properties of glutathione S-transferase from bovine erythrocytes. Protein and peptide letters, 15(1), 6-12, 2008.
  • [23]. Akkemik, E., Taser, R, Bayindir, A., Budak, H., Ciftci, M., Environmental Toxicology an Pharmacology, 34, 888-894, 2012.
  • [24]. Chun-hua, Z., Ze-ying, W., Ting, J., Ying, G. Rice Science, 20(3), 173-178, 2013.
  • [25]. Toribio, F., Martinez-Lara, E., Pascual, P., Lopez-Barea, J. Methods for purification of glutathione peroxidase and related enzymes. Journal of Chromatography B: Biomedical Sciences and Applications, 684(1), 77-97, 1996.
  • [26]. Simons, P.C., Vander Jagt, D.L. Methods Enzymol, 11 ,235-241, 1981. [27]. Habig, W.H., Pabst, M.J., Jakoby, W.B., Glutathione S-transferases. The first enzymmatic step in mercapturic acid formation. J Biol Chem, 246, 7130-7139, 1974.
  • [28]. Bradford M.A. Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal Biochem., 72, 248-254, 1976.
  • [29]. Awasthi, Y.C, Singh, S.V., Purification and Characterization of a New form of Glutathione S-Transferase from Human-Erythrocytes. Biochem. Biophys. Res. Commun, 125, 1053-1060, 1984.
  • [30]. Demirdag, R., Yerlikaya, E., Kufrevioglu, O.I., Gundogdu C, Purification of Glutathione S-transferase Isoenzymes from Tumour and Nontumour Human Stomach and Inhibitory Effects of Some Heavy Metals on Enzymes Activities, Journal of Enzyme Inhibition and Medicinal Chemistry, 28(5), 911-915, 2013.
Toplam 9 adet kaynakça vardır.

Ayrıntılar

Bölüm Araştırma Makalesi
Yazarlar

Fikret Türkan Bu kişi benim

Neslihan Balcı Bu kişi benim

Halis Şakiroğlu Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2014
Yayımlandığı Sayı Yıl 2014 Cilt: 2 Sayı: 2

Kaynak Göster

APA Türkan, F., Balcı, N., & Şakiroğlu, H. (2014). KARAYEMİŞ MEYVESİNDEN (Laurocerasus officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ. Muş Alparslan Üniversitesi Fen Bilimleri Dergisi, 2(2), 280-288.
AMA Türkan F, Balcı N, Şakiroğlu H. KARAYEMİŞ MEYVESİNDEN (Laurocerasus officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ. MAUN Fen Bil. Dergi. Aralık 2014;2(2):280-288.
Chicago Türkan, Fikret, Neslihan Balcı, ve Halis Şakiroğlu. “KARAYEMİŞ MEYVESİNDEN (Laurocerasus Officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ”. Muş Alparslan Üniversitesi Fen Bilimleri Dergisi 2, sy. 2 (Aralık 2014): 280-88.
EndNote Türkan F, Balcı N, Şakiroğlu H (01 Aralık 2014) KARAYEMİŞ MEYVESİNDEN (Laurocerasus officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ. Muş Alparslan Üniversitesi Fen Bilimleri Dergisi 2 2 280–288.
IEEE F. Türkan, N. Balcı, ve H. Şakiroğlu, “KARAYEMİŞ MEYVESİNDEN (Laurocerasus officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ”, MAUN Fen Bil. Dergi., c. 2, sy. 2, ss. 280–288, 2014.
ISNAD Türkan, Fikret vd. “KARAYEMİŞ MEYVESİNDEN (Laurocerasus Officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ”. Muş Alparslan Üniversitesi Fen Bilimleri Dergisi 2/2 (Aralık 2014), 280-288.
JAMA Türkan F, Balcı N, Şakiroğlu H. KARAYEMİŞ MEYVESİNDEN (Laurocerasus officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ. MAUN Fen Bil. Dergi. 2014;2:280–288.
MLA Türkan, Fikret vd. “KARAYEMİŞ MEYVESİNDEN (Laurocerasus Officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ”. Muş Alparslan Üniversitesi Fen Bilimleri Dergisi, c. 2, sy. 2, 2014, ss. 280-8.
Vancouver Türkan F, Balcı N, Şakiroğlu H. KARAYEMİŞ MEYVESİNDEN (Laurocerasus officinalis Roem.) GLUTATYON S-TRANSFERAZ ENZİMİNİN SAFLAŞTIRILMASI VE KARAKTERİZASYONU VE BAZIPESTİSİTLERİN ENZİM AKTİVİTESİNE İNHİBİSYON ETKİLERİ. MAUN Fen Bil. Dergi. 2014;2(2):280-8.
 Kapak Resmi İndir