Nitrogen use efficiency of Buttum (Pistacia khinjuk Stocks) on MS nutrient medium

Yıl 2024, Cilt: 12 Sayı: 1, 58 - 63, 01.07.2024

Yusuf Ersalı

https://doi.org/10.18586/msufbd.1452686

Öz

P. khinjuk species is a native rootstock plant species of pistachio. The difficulty of seed and stem cuttings propagation of P. khinjuk make shoot micropropagation a good option for this species. In order to make the micropropagation of this species more efficient and economical, the amount of ammonium nitrate, the types and amount of auxin and cytokinin have been optimized. 1650, 825, 412.5 and 206.25 mg/L amounts of NH4NO3 were added to basic nutrient medium of Murashige and Skoog (MS) to investigated propagation and rooting of shoot of P. khinjuk species., Benzylamino purine (BAP), Furfurilaminopurine (Kinetin), 2-Isopentyl Adenine (2-IP), for the shoot micropropagation stage, Naphthalene acetic acid (NAA), Indole butyric acid (IBA) and indole acetic acid (IAA) for the rooting stage of the shoots were used. In shoot micropropagation, the highest number of shoots per explant (1.79), average shoot length (17.10 mm) and total soluble protein (3.05 mg/g) amounts were obtained from MS medium containing 825 mg/L NH4NO3 supplemented with 0.5 mg/L BAP. In rooting of shoot, the highest value of rooting rate (81%), number of roots per explant (4.25), average root length (15.95 mm) and total soluble protein amount (3.25 mg/g) were observed in 0.5 mg/L NAA medium containing 412.5 mg/L NH4NO3.

Anahtar Kelimeler

MS, Ammonium Nitrate, NAA, Soluble Protein

MS besin ortamında Buttumun (Pistacia khinjuk Stocks) azot kullanım verimliliği

Öz

P. khinjuk türü antep fıstığının doğal bir anaç türüdür. P. khinjuk'un tohum ve gövde çelikleriyle çoğaltılmasının zorluğu, sürgünlerin mikroçoğaltılmasını iyi bir seçenek haline getirmektedir. Bu türün mikroçoğaltımını daha verimli ve ekonomik hale getirmek amacıyla amonyum nitrat miktarı, oksin ve sitokinin çeşitleri ve miktarları optimize edilmiştir. P. khinjuk türünün sürgün ucu kültürü ile mikroçoğaltımında Murashige ve Skoog (MS) temel besin ortamına NH4NO3’ın 1650, 825, 412.5 ve 206.25 mg/L düzeylerinde katılmasının sürgün ve kök oluşumu üzerine etkileri araştırılmıştır. Sürgün mikroçoğaltım aşamasında benzilamino pürin (BAP), Furfurilaminopurin (Kinetin), 2-İzopentil Adenin (2-IP), sürgünlerin köklendirilmesi aşamasında, Naftalen asetik asit (NAA), İndol butirik asit (IBA) ve indol asetik asit (IAA) kullanılmıştır. Sürgün mikroçoğaltımında en yüksek eksplant başına düşen sürgün sayısı (1.79), ortalama sürgün uzunluğu (17.10 mm) ve total çözülebilir protein (3.05 mg/g) değerinin 0.5 mg/L BAP destekli 825 mg/L NH4NO3 içeren MS ortamından elde edilmiştir. Sürgünlerin in vitro köklendirilmesinde köklenme oranı (% 81), eksplant başına düşen kök sayısı (4.25), ortalama kök uzunluğu (15.95 mm) ve total çözülebilir protein miktarı (3.25 mg/g) 412.5 mg/L NH4NO3 içeren 0.5 mg/L NAA ortamında en yüksek değerde çıkmıştır.

Anahtar Kelimeler

Amonyum nitrat, çözülebilir ptotein, MS, NAA

Kaynakça

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