Investigation of Antiproliferative Effects of Heracleum persicum Extracts on Glioblastoma Multiforme Cell Line

Yıl 2025, Cilt: 13 Sayı: 1, 64 - 69, 30.06.2025

Kemal Alp Nalcı , Leyla Güven , Ravza Manto , Nefise Şentürk , Gökhan Dervişoğlu , Fethi Ahmet Ozdemir , Umut Furkan Bayram , Muhammed Enes Yılmaz , Seda Nur Yöndeş , Adnan Çetin

https://doi.org/10.18586/msufbd.1660341

Öz

Heracleum persicum (H. percium) is a plant known for its anti-inflammatory, analgesic, anticonvulsant, and antioxidant properties. In this study, the antiproliferative effects of the ethanolic leaf and root extracts of the plant on the human Glioblastoma Multiforme U-87MG cell line were evaluated. The leaf and root extracts were applied at concentrations of 10⁻¹, 10⁻², 10⁻³, 10⁻⁴, 10⁻⁵, and 10⁻⁶ mg/mL for 24 and 72 hours, and cell viability was determined using the MTT assay. The results showed that at a concentration of 10⁻¹ mg/mL for 24 hours, the leaf and root extracts caused cell death at rates of 76% and 81%, respectively. In the 72-hour application, the leaf extract at a concentration of 10⁻¹ mg/mL led to 83% cell death, while the root extract at the same concentration caused 92% cell death. These findings demonstrate that H. persicum extracts exhibit antiproliferative effects on the U-87MG cell line and suggest their potential as therapeutic agents.

Anahtar Kelimeler

Antiproliferative effect , Cancer cells , Glioblastoma multiforme , Heracleum persicum , Plant extracts

Proje Numarası

TUBITAK 2209-A (Project No: 1919B012221674).

Heracleum persicum Ekstraktlarının Glioblastoma Multiforme Hücre Hattı Üzerindeki Antiproliferatif Etkilerinin Araştırılması

Öz

Heracleum persicum (H. percium), anti-inflamatuvar, analjezik, antikonvülzan ve antioksidan özellikleriyle bilinen bir bitkidir. Bu çalışmada, bitkinin etanolik yaprak ve kök ekstrelerinin insan Glioblastoma Multiforme U-87MG hücre hattı üzerindeki antiproliferatif etkileri değerlendirildi. Yaprak ve kök ekstreleri 10⁻¹, 10⁻², 10⁻³, 10⁻⁴, 10⁻⁵ ve 10⁻⁶ mg/mL konsantrasyonlarında 24 ve 72 saat süreyle uygulanarak hücre canlılıkları MTT testi ile belirlendi. Sonuçlar, 10⁻¹ mg/mL konsantrasyonunda 24 saatte yaprak ve kök ekstrelerinin sırasıyla %76 ve %81 oranında hücre ölümüne neden olduğunu gösterdi. 72 saatlik uygulamada, yaprak ekstresinin 10⁻¹ mg/mL konsantrasyonu %83 oranında hücre ölümüne yol açarken, kök ekstresinin 10⁻¹ mg/mL konsantrasyonu %92 oranında hücre ölümüne neden oldu. Bu bulgular, H. persicum ekstrelerinin U-87MG hücre hattında antiproliferatif etki gösterdiğini ve potansiyel terapötik ajanlar olarak değerlendirilebileceğini ortaya koymaktadır.

Anahtar Kelimeler

: Antiproliferatif etki , Bitki ekstraktları , Glioblastoma multiforme , Heracleum persicum , Kanser hücreleri

Proje Numarası

TUBITAK 2209-A (Project No: 1919B012221674).

Kaynakça

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