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RELİKT ENDEMİK LIQUIDAMBAR ORIENTALIS (Miller )’İN HİPOKOTİL VE KOTİLEDON EKSPLANTLARINDAN ADVENTİF SÜRGÜN OLUŞUMU

Yıl 2018, Cilt: 4 Sayı: 2, 137 - 142, 19.12.2018
https://doi.org/10.22531/muglajsci.427722

Öz

Bu çalışmada, Bitki Büyüme Düzenleyicileri (BBD)’nin [ 6-Benzil Amino Pürin (BAP) (0, 1.0, 2.0, 3.0 mg L-1) tek başına ve BAP’ın Naftalen Asetik Asit (NAA) (0, 0.05, 0.10, 0.15 mg L-1) veya İndol Bütirik Asit (IBA) (0, 0.01, 0.05, 0.10 mg L-1)ile kombinasyonları] Liquidambar orientalis (Anadolu sığlası)’in 4-5 haftalık steril fidelerinin hipokotil ve kotiledon eksplantlarından direkt organogenez üzerine etkisi araştırılmıştır. Organogenez için ortama BAP ilavesi gerekli bulunmuş ayrıca, BAP’ın NAA ile kombinasyonları IBA ile kombinasyonlarına göre, gerek sürgün oluşturan eksplant yüzdesi gerekse eksplant başına sürgün sayısı ve sürgünlerin gelişimi bakımından daha iyi sonuç vermiştir. Ortalama en yüksek sürgün oluşturan eksplant yüzdesi (%76.64), 0.10 mg L-1NAA + 3.0 mg L-1BAP içeren Woody Plant Medium (WPM)’da kültüre alınan hipokotil eksplantlarından elde edilmiştir. Eksplant başına ortalama en yüksek sürgün sayısı (32.20 sürgün/eksplant), 0.10 mg L-1IBA + 1.0 mg L-1BAP eklenmiş WPM besin ortamında kültüre alınan hipokotil eksplantlarında gözlenmiştir. Her iki eksplant için de 0.05 mg L-1NAA’in 1.0 veya 2.0 mg L-1BAP ile kombinasyonu uygun bulunmuştur.

Kaynakça

  • [1] Ickert-Bond, S. M.; Pigg, K. B. and Wen J., Comparative ınfructescence morphology in Liquidambar (Altingiaceae) and its evolutionary significance. American Journal of Botany, 92(8): 1234-1255, 2005.
  • [2] Öztürk, M.; Çelik, A.; Güvensen, A. and Hamzaoğlu, E., Ecology of tertiary relict endemic Liquidambar orientalis Mill. Forests. Forest Ecology and Management, 256: 510-518, 2008.
  • [3] Atay, İ., Sığla ağacının (Liquidambar orientalis Mill.) önemi ve silvikültür özellikleri. (Importance and silvicultural properties of the sweetgum tree Liquidambar orientalis Mill.). İstanbul Üniversitesi Orman Fakültesi Dergisi, 35(1): 15-21, 1985.
  • [4] Velioğlu, E.; Kandemir, G.; Tayanç, Y.; Çengel, B.; Alan, M. and Kaya, Z., Türkiye’deki sığla Liquidambar orientalis Miller populasyonlarının genetik yapısının moleküler belirteçlerle belirlenmesi ve koruma stratejileri geliştirilmesi. (Determination of Genetic Diversity and Gene Conservation Strategies for Oriental Sweetgum (Liquidambar orientalis Miller) Populations in Turkey by Molecular Markers). T.C. Çevre ve Orman Bakanlığı, Orman Ağaçları ve Tohumları Islah Araştırma Müdürlüğü, Teknik Bülten No:20, Bakanlık Yayın No:339, Pp.43, Ankara-Türkiye, 2008.
  • [5] Prada, M. A. and Arizpe, D., Riparian Tree and Shrub Propagation Handbook. An Aid to Riverine Restoration in the Mediterranean Region. Generalitat- Valenciana, 2008.
  • [6] Parlak S., Sığla (Liquidambar orientalis Miller)’da alternatif vegetatif üretim yöntemi. (Alternative vegetative production method in sweetgum (Liquidambar orientalis Miller)). Orman Mühendisliği, 49(10-11-12): 20-23, 2012.
  • [7] Genç, A., Sığla ağacı (Liquidambar orientalis Mill.)’nın doku kültürü tekniği ile üretilmesi. (Propagation of Liquidambar orientalis Mill. Using Tissue Culture Technique). T.C. Orman Bakanlığı, Ege Ormancılık Araştırma Enstitüsü Müdürlüğü, Teknik Bülten No:14, Pp.57, İzmir-Türkiye, 1999.
  • [8] Erdağ, B. and Emek, Y., In vitro adventitious shoot regeneration of Liquidambar orientalis Miller. Journal of Biological Sciences, 5(6): 805-808, 2005.
  • [9] Özmen, K., Sığla ağacının (Liquidambar orientalis Miller) in vitro koşullarda çoğaltım özelliklerinin araştırılması. (Investigation of Anatolian sweetgum tree’s (Liquidambar orientalis Miller) propagation properties under in vitro conditions). Yüksek Lisans Tezi, Pp.48, Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Antalya-Turkiye, 2011.
  • [10] Bayraktar, M.; Hayta, Ş.; Parlak, S. and Gürel, A., Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots. Trees, 29: 999-1009, 2015.
  • [11] Surgun-Acar, Y. and Bürün, B., Use of tissue Culture techniques in conservation of sweetgum. VIII. International Symposium on Ecology and Enviromental Problems (ISEEP 2017), 4-7 October 2017, Çanakkale-Turkey, Abstract Book p.251, 2017.
  • [12] Sommer, H. E. and Brown C. L., Notes: Embryogenesis in tissue cultures of sweetgum. Forest Science, 26(2): 257-260, Abstract, 1980.
  • [13] Sommer, H. E., Propagation of sweetgum by tissue culture. Reforestation, Nurseries & Genetic Resources (RNGR), Tree Improvement and Genetics. Southern Forest Tree Improvement Conference-1981, Section 3: The Role and Potential for Vegetative Propagation in Southern Forestry, 1981.
  • [14] Kim, M. K.; Sommer, H. E.; Bongarten, B. C. and Merkle, S. A., High-frequency induction of adventitious shoots from hypocotyl segments of Liquidambar styraciflua L. thidiazuron. Plant Cell Reports, 16: 536-540, 1997.
  • [15] Sutter, E. G. and Barker, P. B., In vitro propagation of mature Liquidambar styraciflua. Plant Cell Tissue Organ Culture, 5: 13-21, 1985.
  • [16] Brand, M. H. and Lineberger, R. D., In vitro adventitious shoot formation on mature-phase leaves and petioles of Liquidambar styraciflua L. Plant Science, 57(2): 173-179, 1988.
  • [17] Brand, M. H. and Lineberger, R. D., The effect of leaf source and developmental stage on shoot organogenic potential of sweetgum (Liquidambar styraciflua L.) leaf explants. Plant Cell, Tissue and Organ Culture, 24: 1-7, 1991.
  • [18] Merkle, S. A., Neu, K. A., Battle, P. J. and Bailey, R. L., Somatic embryogenesis and plantlet regeneration from immature and mature tissues of sweetgum (Liquidambar styraciflua). Plant Science, 132: 169-178, 1998.
  • [19] Merkle, S. A. and Battle, P. J., Enhancement of embryogenic culture initiation from tissues of mature sweetgum trees. Plant Cell Reports, 19: 268-273, 2000.
  • [20] Su, M-Y., Tissue culture of stem segments and plantlet regeneration of Liquidambar styraciflua. Forest Research, Abstract 2005-01, 2005.
  • [21] Ďurkovič, J. and Lux, A., Micropropagation with a novel pattern of adventitious rooting in American sweetgum (Liquidambar styraciflua L.). Trees, 24: 491-497, 2010.
  • [22] Baran-Ayaz, Ö.; Acar-Surgun, Y. and Bürün, B., Endemik Anadolu sığlası (Liquidambar orientalis Mill.)’nda tohum sterilizasyonu ve çimlenme. (Seed sterilization and germination of endemic Oriental sweetgum Liquidambar orientalis Mill.). Journal of Anatolian Natural Sciences, 6: 82-86, 2015.
  • [23] Murashige, T. and Skoog, F. A., A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15: 473-479, 1962.
  • [24] Lloyd, G. B. and McCown, B. H., Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Int. Plant Prop. Soc., 30: 421-427, 1980. [25] Merkle, S. A.; Battle, P. J. and Ware, G. O., Factors influencing production of inflorescence-derived somatic seedlings of sweetgum. Plant Cell, Tissue and Organ Culture, 73: 95-99, 2003.
  • [26] Xu, L.; Liu, G. F. and Bao, M. Z., Adventitious shoot regeneration from ın vitro leaves of formosan sweetgum (Liquidambar formosana L.). HortScience, 42(3): 721-723, 2007.

ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller

Yıl 2018, Cilt: 4 Sayı: 2, 137 - 142, 19.12.2018
https://doi.org/10.22531/muglajsci.427722

Öz

In this study, the effect of Plant Growth Regulators (PGRs) [6-Benzylaminopurine (BAP)(0, 1.0, 2.0, 3.0 mg L-1) alone and BAP in combinations with α-Naphthaleneacetic acid (NAA)(0, 0.05, 0.10, 0.15 mg L-1) or Indole-3-butyric acid (IBA)(0, 0.01, 0.05, 0.10 mg L-1)] on direct organogenesis from hypocotyl and cotyledon explants of 4-5-week-old sterile seedlings of Liquidambar orientalis (Oriental sweetgum) were investigated. For organogenesis, the addition of BAP to the medium was required, also the combinations of BAP with NAA responded better in terms of the percentage of shoot-forming explants, the number of shoots per explant and the growth of shoots as compared to combinations of BAP with IBA. The highest average percentage of shoot formation (76.64%) was obtained from hypocotyl explants cultured on Woody Plant Medium (WPM) containing 0.10 mg L-1NAA + 3.0 mg L-1BAP. The highest average shoot number per explant (32.20 shoots/explant) was observed in also hypocotyl explants cultured on WPM supplemented with 0.10 mg L-1IBA + 1.00 mg L-1BAP. The combination of 0.05 mg L-1NAA + 1.0 or 2.0 mg L-1BAP was found to be appropriate for both explants.

Kaynakça

  • [1] Ickert-Bond, S. M.; Pigg, K. B. and Wen J., Comparative ınfructescence morphology in Liquidambar (Altingiaceae) and its evolutionary significance. American Journal of Botany, 92(8): 1234-1255, 2005.
  • [2] Öztürk, M.; Çelik, A.; Güvensen, A. and Hamzaoğlu, E., Ecology of tertiary relict endemic Liquidambar orientalis Mill. Forests. Forest Ecology and Management, 256: 510-518, 2008.
  • [3] Atay, İ., Sığla ağacının (Liquidambar orientalis Mill.) önemi ve silvikültür özellikleri. (Importance and silvicultural properties of the sweetgum tree Liquidambar orientalis Mill.). İstanbul Üniversitesi Orman Fakültesi Dergisi, 35(1): 15-21, 1985.
  • [4] Velioğlu, E.; Kandemir, G.; Tayanç, Y.; Çengel, B.; Alan, M. and Kaya, Z., Türkiye’deki sığla Liquidambar orientalis Miller populasyonlarının genetik yapısının moleküler belirteçlerle belirlenmesi ve koruma stratejileri geliştirilmesi. (Determination of Genetic Diversity and Gene Conservation Strategies for Oriental Sweetgum (Liquidambar orientalis Miller) Populations in Turkey by Molecular Markers). T.C. Çevre ve Orman Bakanlığı, Orman Ağaçları ve Tohumları Islah Araştırma Müdürlüğü, Teknik Bülten No:20, Bakanlık Yayın No:339, Pp.43, Ankara-Türkiye, 2008.
  • [5] Prada, M. A. and Arizpe, D., Riparian Tree and Shrub Propagation Handbook. An Aid to Riverine Restoration in the Mediterranean Region. Generalitat- Valenciana, 2008.
  • [6] Parlak S., Sığla (Liquidambar orientalis Miller)’da alternatif vegetatif üretim yöntemi. (Alternative vegetative production method in sweetgum (Liquidambar orientalis Miller)). Orman Mühendisliği, 49(10-11-12): 20-23, 2012.
  • [7] Genç, A., Sığla ağacı (Liquidambar orientalis Mill.)’nın doku kültürü tekniği ile üretilmesi. (Propagation of Liquidambar orientalis Mill. Using Tissue Culture Technique). T.C. Orman Bakanlığı, Ege Ormancılık Araştırma Enstitüsü Müdürlüğü, Teknik Bülten No:14, Pp.57, İzmir-Türkiye, 1999.
  • [8] Erdağ, B. and Emek, Y., In vitro adventitious shoot regeneration of Liquidambar orientalis Miller. Journal of Biological Sciences, 5(6): 805-808, 2005.
  • [9] Özmen, K., Sığla ağacının (Liquidambar orientalis Miller) in vitro koşullarda çoğaltım özelliklerinin araştırılması. (Investigation of Anatolian sweetgum tree’s (Liquidambar orientalis Miller) propagation properties under in vitro conditions). Yüksek Lisans Tezi, Pp.48, Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Antalya-Turkiye, 2011.
  • [10] Bayraktar, M.; Hayta, Ş.; Parlak, S. and Gürel, A., Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots. Trees, 29: 999-1009, 2015.
  • [11] Surgun-Acar, Y. and Bürün, B., Use of tissue Culture techniques in conservation of sweetgum. VIII. International Symposium on Ecology and Enviromental Problems (ISEEP 2017), 4-7 October 2017, Çanakkale-Turkey, Abstract Book p.251, 2017.
  • [12] Sommer, H. E. and Brown C. L., Notes: Embryogenesis in tissue cultures of sweetgum. Forest Science, 26(2): 257-260, Abstract, 1980.
  • [13] Sommer, H. E., Propagation of sweetgum by tissue culture. Reforestation, Nurseries & Genetic Resources (RNGR), Tree Improvement and Genetics. Southern Forest Tree Improvement Conference-1981, Section 3: The Role and Potential for Vegetative Propagation in Southern Forestry, 1981.
  • [14] Kim, M. K.; Sommer, H. E.; Bongarten, B. C. and Merkle, S. A., High-frequency induction of adventitious shoots from hypocotyl segments of Liquidambar styraciflua L. thidiazuron. Plant Cell Reports, 16: 536-540, 1997.
  • [15] Sutter, E. G. and Barker, P. B., In vitro propagation of mature Liquidambar styraciflua. Plant Cell Tissue Organ Culture, 5: 13-21, 1985.
  • [16] Brand, M. H. and Lineberger, R. D., In vitro adventitious shoot formation on mature-phase leaves and petioles of Liquidambar styraciflua L. Plant Science, 57(2): 173-179, 1988.
  • [17] Brand, M. H. and Lineberger, R. D., The effect of leaf source and developmental stage on shoot organogenic potential of sweetgum (Liquidambar styraciflua L.) leaf explants. Plant Cell, Tissue and Organ Culture, 24: 1-7, 1991.
  • [18] Merkle, S. A., Neu, K. A., Battle, P. J. and Bailey, R. L., Somatic embryogenesis and plantlet regeneration from immature and mature tissues of sweetgum (Liquidambar styraciflua). Plant Science, 132: 169-178, 1998.
  • [19] Merkle, S. A. and Battle, P. J., Enhancement of embryogenic culture initiation from tissues of mature sweetgum trees. Plant Cell Reports, 19: 268-273, 2000.
  • [20] Su, M-Y., Tissue culture of stem segments and plantlet regeneration of Liquidambar styraciflua. Forest Research, Abstract 2005-01, 2005.
  • [21] Ďurkovič, J. and Lux, A., Micropropagation with a novel pattern of adventitious rooting in American sweetgum (Liquidambar styraciflua L.). Trees, 24: 491-497, 2010.
  • [22] Baran-Ayaz, Ö.; Acar-Surgun, Y. and Bürün, B., Endemik Anadolu sığlası (Liquidambar orientalis Mill.)’nda tohum sterilizasyonu ve çimlenme. (Seed sterilization and germination of endemic Oriental sweetgum Liquidambar orientalis Mill.). Journal of Anatolian Natural Sciences, 6: 82-86, 2015.
  • [23] Murashige, T. and Skoog, F. A., A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15: 473-479, 1962.
  • [24] Lloyd, G. B. and McCown, B. H., Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Int. Plant Prop. Soc., 30: 421-427, 1980. [25] Merkle, S. A.; Battle, P. J. and Ware, G. O., Factors influencing production of inflorescence-derived somatic seedlings of sweetgum. Plant Cell, Tissue and Organ Culture, 73: 95-99, 2003.
  • [26] Xu, L.; Liu, G. F. and Bao, M. Z., Adventitious shoot regeneration from ın vitro leaves of formosan sweetgum (Liquidambar formosana L.). HortScience, 42(3): 721-723, 2007.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Yonca Surgun Acar 0000-0002-8684-329X

Özlem Baran Ayaz Bu kişi benim 0000-0003-1765-5022

Betül Bürün 0000-0002-3758-0630

Yayımlanma Tarihi 19 Aralık 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 4 Sayı: 2

Kaynak Göster

APA Surgun Acar, Y., Ayaz, Ö. B., & Bürün, B. (2018). ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller. Mugla Journal of Science and Technology, 4(2), 137-142. https://doi.org/10.22531/muglajsci.427722
AMA Surgun Acar Y, Ayaz ÖB, Bürün B. ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller. MJST. Aralık 2018;4(2):137-142. doi:10.22531/muglajsci.427722
Chicago Surgun Acar, Yonca, Özlem Baran Ayaz, ve Betül Bürün. “ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller”. Mugla Journal of Science and Technology 4, sy. 2 (Aralık 2018): 137-42. https://doi.org/10.22531/muglajsci.427722.
EndNote Surgun Acar Y, Ayaz ÖB, Bürün B (01 Aralık 2018) ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller. Mugla Journal of Science and Technology 4 2 137–142.
IEEE Y. Surgun Acar, Ö. B. Ayaz, ve B. Bürün, “ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller”, MJST, c. 4, sy. 2, ss. 137–142, 2018, doi: 10.22531/muglajsci.427722.
ISNAD Surgun Acar, Yonca vd. “ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller”. Mugla Journal of Science and Technology 4/2 (Aralık 2018), 137-142. https://doi.org/10.22531/muglajsci.427722.
JAMA Surgun Acar Y, Ayaz ÖB, Bürün B. ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller. MJST. 2018;4:137–142.
MLA Surgun Acar, Yonca vd. “ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller”. Mugla Journal of Science and Technology, c. 4, sy. 2, 2018, ss. 137-42, doi:10.22531/muglajsci.427722.
Vancouver Surgun Acar Y, Ayaz ÖB, Bürün B. ADVENTITIOUS SHOOT FORMATION FROM HYPOCOTYL AND COTYLEDON EXPLANTS OF RELICT ENDEMIC LIQUIDAMBAR ORIENTALIS Miller. MJST. 2018;4(2):137-42.

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