Kurkumin ve Naringenin’in Bakır Nanopartikülleri ile Oluşturulmuş Karaciğer Hasarı Üzerine Etkilerinin İncelenmesi

Yıl 2023, , 107 - 117, 27.01.2023

Hiba Lalou , Metin Yıldırım , Merih Akkapulu , Serap Yalın , Ali Yalın

https://doi.org/10.31020/mutftd.1186446

Öz

Giriş: Bakır nanopartiküllerinin (CuNP) günümüzde kullanım sıklığındaki artış insanların bu maddeye olan maruziyetin artması ile sonuçlanmaktadır. Curcuma longa L. ve Naringenin kanser türlerinin oluşma riskinin azaltmakta ve insanlarda koruyucu biyolojik etkilere yardımcı olmaktadır.
Amaç: Çalışmamızda, bakır nanopartiküller ile oluşturulmuş oksidatif strese karşı kurkumin ve naringeninin olası koruyucu etkileri incelenmiştir.
Yöntem: Çalışmamızda 42 rat 6 gruba ayrılmıştır. Gruplardan biri kontrol grubu iken, diğer gruplara farklı dozlarda kurkumin ve naringenin maddeleri ile CuNP uygulanmıştır. Çalışma sonunda karaciğer dokusu izole edildi ve Süperoksit Dismutaz (SOD), Katalaz (KAT) aktiviteleri, Glutatyon (GSH), Malondialdehit (MDA) düzeyleri ve inflamasyon belirteçleri (IL-1α, IL-1β) araştırılmıştır.
Bulgular: Elde edilen bulgular sonucunda, bakır nanopartiküllerin oksidatif stresi arttırdığı ve antioksidan enzim seviyelerinde düşüşe sebep olduğu; kurkumin ve naringeninin ise oksidatif stresi azalttığı ve antioksidan enzim seviyelerini arttırdığı saptanmıştır. Karaciğer dokusunda IL-1α parametresi incelendiğinde, kontrol grubu ve farklı dozlarda kurkumin ve naringenin CuNP ile karşılaştırıldığında, IL-1α düzeyinde azalma saptanmıştır. Bu düşüş istatistiksel olarak anlamlı değildi. IL-1β parametresi, kontrol grubuna kıyasla CuNP grubunda istatistiksel olarak anlamlı bir artış gösterdi (p<0.05). IL-1β düzeyi incelendiğinde diğer gruplara kıyasla CuNP grubunda artma saptanmıştır.
Sonuç: İnsanların bakır nanopartiküllere maruz kalması durumunda oluşabilecek zararlı etkilerden korunma ve tedavi için kurkumin ve naringenin kullanılabileceği düşünülebilir.

Anahtar Kelimeler

Bakır nanopartikül, karaciğer, oksidatif stres, kurkumin, naringenin

Destekleyen Kurum

Mersin Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

2020-1-TP2-4062

Teşekkür

Bu çalışma, 2020-1-TP2-4062 numaralı proje kapsamında Mersin Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir.

Study The Effects of Curcumın and Naringenın on Liver Damage Caused by Copper Nanoparticles

Öz

Introduction: The increase in the frequency of use of copper nanoparticles (CuNP) results in an increased exposure of humans to this substance. Curcuma longa L. and Naringenin consumption reduces the risk of developing cancer types and helps protective biological effects in humans.
Aim: In our study, the possible effects of different doses of curcumin and naringenin on the application of copper nanoparticles, oxidative stress and inflammation were investigated.
Methods: In this study, 42 rats were divided into 6 groups. One of the groups was the control group, while the other groups were administered different doses of curcumin and naringenin substances as well as CuNP. At the end of the study, after the liver tissue was isolated and Superoxide dismutase (SOD), Catalase (CAT), Glutathione (GSH), Malondialdehyde (MDA) activities and inflammation markers (IL-1α, IL-1β) were investigated.
Result: As a result, it was found that copper nanoparticles increased oxidative stress and decreased antioxidant enzyme activities, while curcumin and naringenin decreased oxidative stress and increased antioxidant enzyme levels. When the IL-1α parameter in liver tissue was examined, the level of IL-1α decreased, this decrease was not statistically significant. The IL-1β parameter showed a statistically significant increase in the CuNP group compared to the control group (p<0.05). IL-1β level was decreased in different doses of curcumin and naringenin groups compared to CuNP group (p<0.05).
Conclusion: It can be thought that curcumin and naringenine can be used for the protection and treatment against detrimental effects that may occur in case of exposure to copper nanoparticles in humans.

Anahtar Kelimeler

Copper nanoparticles, liver, oxidative stress, curcumine, naringenin

Proje Numarası

2020-1-TP2-4062

Kaynakça

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