Effect of Sour Cherry Fruit on Urease, Chymotrypsin and Trypsin Enzymes

Öz

Aim: The aim of this study was to determine urease, chymotrypsin, and trypsin inhibitory activity of sour cherry. Method: In this study, which was conducted between July 2024 and January 2025, 80% ethanol extract was prepared from sour cherry fruits collected from Çubuk district (Ankara), and fractionated with n-hexane, chloroform, ethyl acetate, and n-butanol. Then, urease, chymotrypsin, and trypsin inhibitory activities of extracts and fractions were determined. Additionally, phenolic content of ethanol extract and ethyl acetate fraction, the strongest activity was observed, were analyzed by HPLC. Results: Ethyl acetate, n-butanol, and remaining water fractions inhibited trypsin enzyme by more than 95%. Similarly, the strongest chymotrypsin and urease inhibitory activity was observed in ethyl acetate fraction (%84,07 and 42,36% inhibition, respectively). HPLC analysis revealed that ethanol extract contained 0,1082% and 0,0512% chlorogenic acid and rutin, respectively, while ethyl acetate fraction contained 0,165% and 0,079% chlorogenic acid and rutin, respectively. Conclusion: The inhibitory effects of ethanol extract and different fractions obtained from sour cherry fruit against urease, chymotrypsin, and trypsin were evaluated for the first time. The findings revealed that especially ethyl acetate fraction showed high inhibitory activity on these enzymes. HPLC analysis shows that chlorogenic acid and rutin are present in ethanol extract and ethyl acetate fraction, and these compounds may contribute to inhibitory activity. Accordingly, it was thought that sour cherry fruit could be evaluated among natural urease and serine protease inhibitor candidates that can be used in prevention or treatment of ulcers, obesity, and inflammatory diseases such as pancreatitis.

Anahtar Kelimeler

• Chymotrypsin
• Prunus cerasus
• sour cherry
• trypsin
• urease

Vişne Meyvesinin Üreaz, Kimotripsin ve Tripsin Enzimleri Üzerine Etkisi

Öz

Amaç: Bu araştırmada vişne meyvesinin (Prunus cerasus L.) üreaz, kimotripsin ve tripsin inhibe edici aktivitesinin tespiti amaçlanmıştır. Yöntem: 2024 yılı Temmuz ve 2025 Ocak ayları arasında gerçekleştirilen bu çalışmada Ankara’nın Çubuk ilçesinden toplanan vişne meyvelerinden %80 etanol ekstresi hazırlanmış ve hazırlanan ekstre sırasıyla n-hekzan, kloroform, etil asetat ve n-bütanol ile fraksiyonlanmıştır. Daha sonra elde edilen ekstre ve fraksiyonların in vitro üreaz, kimotripsin ve tripsin inhibe edici aktiviteleri belirlenmiştir. Ayrıca etanol ekstresi ve en kuvvetli aktivitenin gözlendiği etil asetat fraksiyonunun fenolik içeriği YPSK (Yüksek Performanslı Sıvı Kromatografisi) ile analiz edilmiştir. Bulgular: Etil asetat, n-bütanol ve kalan su fraksiyonları tripsin enzimini %95’ten yüksek oranlarda inhibe etmiştir. Yine benzer şekilde en kuvvetli kimotripsin ve üreaz inhibe edici aktivite etil asetat fraksiyonunda gözlemlenmiştir (sırasıyla %84,07 ve %42,36 inhibisyon). YPSK analizi ile etanol ekstresinin sırasıyla %0,1082 ve %0,0512 oranlarında; etilasetat fraksiyonunun ise sırasıyla %0,165 ve %0,079 oranında klorojenik asit ve rutin içerdiği tespit edilmiştir. Sonuç: Vişne meyvesinden elde edilen %80 etanol ekstresi ve farklı fraksiyonların üreaz, kimotripsin ve tripsin enzimlerine karşı inhibitör etkileri ilk kez değerlendirilmiştir. Elde edilen bulgular, özellikle etil asetat fraksiyonunun bu enzimler üzerinde yüksek inhibe edici aktivite gösterdiğini ortaya koymuştur. YPSK analizi hem etanol ekstresinde hem de etil asetat fraksiyonunda klorojenik asit ve rutin gibi fenolik bileşiklerin bulunduğunu ve bu bileşiklerin inhibisyon aktivitesine katkı sağlayabileceğini göstermiştir. Bu doğrultuda, vişne meyvesinin ülser, obezite ve pankreatit gibi inflamatuvar hastalıkların önlenmesi veya tedavisinde kullanılabilecek doğal üreaz ve serin proteaz inhibitörü adayları arasında değerlendirilebileceği düşünülmüştür.

Anahtar Kelimeler

• Kimotripsin
• Prunus cerasus
• Rutin
• Tripsin
• Üreaz

Kaynakça

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