Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey

Merve Koçak; Yavuz Bağcı

TR EN

Türkiye’ye endemik Stachys gaziantepensis ve Stachys ketenoglui (Lamiaceae) türlerinin antioksidan aktivitesi ve tirozinaz enzim inhibisyon potansiyeli

Öz

Bu çalışmada, Türkiye’ye endemik iki tür olan Stachys gaziantepensis ve Stachys ketenoglui’nin fitokimyasal profili ve biyolojik aktiviteleri araştırılmıştır. Bitkilerin toprak üstü kısımlarından ultrason destekli ekstraksiyon yöntemi ile elde edilen etil asetat, metanol ve su ekstraktlarının; toplam fenolik ve flavonoid içerikleri, antioksidan aktiviteleri (DPPH, ABTS ve metal şelatlama testleri) ile tirozinaz enzim inhibisyon potansiyeli açısından değerlendirilmiştir. Elde edilen bulgulara göre, en yüksek fenolik ve flavonoid içerik S. gaziantepensis’in etil asetat ekstraktında belirlenmiştir. Her iki türün metanol ekstraktları DPPH ve ABTS testlerinde en güçlü radikal süpürücü aktiviteyi göstermiştir. Metal şelatlama kapasitesinin ise S. ketenoglui’nin etil asetat ekstraktında en yüksek olduğu tespit edilmiştir. Ayrıca, S. gaziantepensis’in etil asetat ekstraktı dikkat çekici düzeyde tirozinaz inhibisyon aktivitesi sergilemiştir. Genel olarak, her iki bitki türünün de önemli biyolojik aktivitelerinin olduğu, ancak S. gaziantepensis’in daha yüksek biyolojik potansiyel gösterdiği belirlenmiştir. Bulgular ayrıca, çözücü polaritesinin biyolojik olarak aktif bileşiklerin ekstraksiyonunda önemli bir rol oynadığını ortaya koymakta olup, bu endemik türlerin farmasötik ve kozmetik uygulamalar açısından umut verici doğal kaynaklar olabileceğini göstermektedir.

Anahtar Kelimeler

Antioksidan aktivite , Stachys gaziantepensis , Stachys ketenoglui , Tirozinaz enzim inhibisyonu

Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey

Abstract

This study investigates the phytochemical profile and biological activities of two endemic Turkish species, Stachys gaziantepensis and Stachys ketenoglui. Aerial parts of the plants were extracted using ethyl acetate, methanol, and water through an ultrasound-assisted extraction process. The resulting extracts were evaluated in terms of total phenolic and flavonoid contents, antioxidant capacity (DPPH, ABTS, and metal chelation assays), and tyrosinase inhibitory activity. Among the tested samples, the ethyl acetate extract of S. gaziantepensis exhibited the highest phenolic and flavonoid levels. Methanol extracts of both species demonstrated superior radical scavenging performance in DPPH and ABTS assays, whereas the strongest metal-chelating effect was observed in the ethyl acetate extract of S. ketenoglui. Notably, the ethyl acetate extract of S. gaziantepensis showed pronounced tyrosinase inhibition. Overall, the findings indicate that both species possess considerable bioactive potential, with S. gaziantepensis displaying comparatively stronger activity. The results also emphasize the influence of solvent polarity on the extraction of biologically active compounds, suggesting that these endemic taxa may serve as promising candidates for pharmaceutical and cosmetic applications.

Keywords

Antioxidant activity , Stachys gaziantepensis , Stachys ketenoglui , Tyrosinase enzyme inhibition

Kaynakça

Faydaoğlu, E., and Sürücüoğlu, M. S., (2011). “Geçmişten günümüze tıbbi ve aromatik bitkilerin kullanılması ve ekonomik önemi,” Kastamonu University Journal of Forestry Faculty, vol. 11, no. 1, pp. 52–67. https://izlik.org/JA42YU59AX
Güner, A., Kandemir, A., Menemen, Y., Yıldırım, H., Aslan, S., Ekşi, G., Güner, I., and Çimen, A., (2014). “Resimli Türkiye Florası”.
Carrick, J., (1976). “Studıes In Australıan Lamıaceae 1. The Genus Wrıxonıa F. Muell. (Prostantheroıdeae),” Journal of the Adelaide Botanic Garden. https://www.jstor.org/stable/23872061
Skaltsa, H., Demetzos, C., Lazari, D., and Sokovic, M., (2003). “Essential oil analysis and antimicrobial activity of eight Stachys species from Greece,” Phytochemistry. https://doi.org/10.1016/S0031-9422(03)00386-8
Akçay S., and Şengül, H., (2023). “A Study on Environmental Literacy of Middle School Students,” Necmettin Erbakan Üniversitesi Ahmet Keleşoğlu Eğitim Fakültesi Dergisi, vol. 5, no. 1, pp. 139–169. https://doi.org/10.38151/akef.2023.48
Bahadori, M., B., Kirkan, B., and Sarıkürkçü, C., (2019). “Phenolic ingredients and therapeutic potential of Stachys cretica subsp. smyrnaea for the management of oxidative stress, Alzheimer’s disease,” Ind Crops Prod, vol. 127, pp. 82–87. https://doi.org/10.1016/j.indcrop.2018.10.066
Bahadori, M., B., Kirkan, B., Sarıkürkçü C., and Ceylan, O., (2019). “Metabolite profiling and health benefits of Stachys cretica subsp. mersinaea as a medicinal food,” Ind Crops Prod, vol. 131, pp. 85–89. https://doi.org/10.1016/j.indcrop.2019.01.038
Temel, M., Kargıoğlu, M., Arı, S., (2015). “Afyonkarahisar’da yayılış gösteren Stachys byzantina (Lamiaceae)’nın morfolojik, anatomik ve ekolojik özellikleri,” Süleyman Demirel University Faculty of Arts and Science Journal of Science, vol. 10, no. 2, pp. 35–47. https://izlik.org/JA97EF62BN
Topçu, G., (2019). “Chemical Diversity of Bioactive Diterpenoids of Endemic Anatolian Lamiaceae Plants,” Mesmap–5 Proceedings Book.
Bhattacharjee, R., “Taxonomic studies in Stachys. II. A new infrageneric classification of Stachys L.,” Notes from the Royal Botanic Garden Edinburgh, 1980.

Piozzi, F., and Bruno, M., (2009). “Diterpenoids in the essential oils from the genus Stachys,” Records of Natural Products, vol. 3, pp. 120–125. EISSN: 1307-6167.
Gören, A., C., (2014). “Use of Stachys species (mountain tea) as herbal tea and food,” Records of Natural Products, vol. 8, no. 2, pp. 71–82. EISSN:1307-6167.
Doğan F., and Keleş, Y., (2020). “Ortaokul ve lise öğrencilerinde çevre farkındalığı ve çevre davranışı,” Necmettin Erbakan Üniversitesi Ereğli Eğitim Fakültesi Dergisi, vol. 2–1. https://izlik.org/JA52JA48ZE
Gümüş, N., E., Keskinkaya, H., B., Okudan, E., Ş., and Akköz, C., (2024). “Akdeniz Bölgesi’nden (Antalya/Türkiye) Alınan Makroalg Türlerinin Makro ve İz Element Seviyelerinin Belirlenmesi,” Necmettin Erbakan Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, vol. 6 (1), pp. 1–9. https://doi.org/10.47112/neufmbd.2024.28
Akçiçek, E., (2012). “Stachys L, Türkiye Bitkileri Listesi (Damarlı Bitkiler),” Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.
Güner Ö., and Akçiçek, E., (2015). “Türkiye florası için yeni bir kayıt: Stachys megalodanta Hausskn. & Bornm. ex PH Davis subsp. megalodanta Lamiacease,” Bağbahçe Bilimi, p. 27. https://izlik.org/JA46ZP27RK
Salmaki, Y., Zarre, S., Govaerts, R., and Bräuchler, C., (2012). “A taxonomic revision of the genus Stachys (Lamiaceae: Lamioideae) in Iran,” vol. 170, no. 4, pp. 573–617. https://doi.org/10.1111/j.1095-8339.2012.01317.x
Güner A., and Aslan, S., (2012). “Türkiye Bitkileri Listesi (Damarlı Bitkiler),” Nezahat Gökyiǧit Botanik Bahçesi Yayınları.
Husen A., and Iqbal, M., (2019). “Nanomaterials and Plant Potential: An Overview,” Nanomaterials and Plant Potential, pp. 3–29.
Tundis, R., Bonesi, M., Pugliese, A., Nadjafi, F., Menichini, F., and Loizzo, M., R., (2015). “Tyrosinase, acetyl-and butyryl-cholinesterase inhibitory activity of Stachys lavandulifolia Vahl (Lamiaceae) and its major constituents,” vol. 9, pp. 81–93. EISSN: 1307-6167
Tundis, R., Peruzzi, L., and Menichini F., (2014). “Phytochemical and biological studies of Stachys species in relation to chemotaxonomy: A review,” Phytochemistry. https://doi.org/10.1016/j.phytochem.2014.01.023
Keskinkaya, H., B., Argon, Z., Ü., Doğu, S., and Akdağ, T., (2023). “Phenolic Content and In-vitro Antioxidant Activity of Olea europaea L. subs. oleaster Leaves by Supercritical CO2 Extraction,” Necmettin Erbakan Üniversitesi Ereğli Tarım Bilim. Dergisi. http://dx.doi.org/10.54498/ETBD.2023.25
Erkan T., D., and Dülger, B., (2016). “Stachys thirkei Bitkisinin Antimikrobiyal Aktivitesi Üzerine Araştırmalar,” Düzce Üniversitesi Bilim ve Teknoloji Dergisi, vol. 4, pp. 886–893. https://izlik.org/JA86MJ28JD
Zargari, A., (1990). “Herbal Medicines,” Publication of Tehran University”, Tehran, 83.
Pirbaloutl, A., (2009). “Medicinal plants used in Chaharmahal and Bakhtyari districts of Iran” ISSN (Electronic): 2449-8343
Kumar, D., Bhat, Z. A., Kumar, V., Khan, N., Chashoo, I., Zargar, M. ve Shah, M., (2012). “Effects of Stachys tibetica essential oil in anxiety,” Elsevier, 4 (2), e169-e176. https://doi.org/10.1016/j.eujim.2012.01.005Get rights and content
Sayar, E., H., (2020). “Aeroallergen Sensitivity of Atopic Children in Alanya Region.,” NEÜ PRESS-Selcuk University Medical Journal, vol. 3, p. 36. DOI: 10.30733/std.2020.01390
Karaoğlan, E., S., Köseğlu, M., Ş., Öztürk, M., and Kaya, G., (2018). “Erzurum’da yetişen ve halk arasında tıbbi önemi olan bazı bitki türleri,” Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik. https://doi.org/10.31020/mutftd.428960
Kaya, A., Demirci, B., Doğu, S., and Dinç, M., (2017). “Composition of the essential oil of Stachys sericantha, S. gaziantepensis, and S. mardinensis (Lamiaceae) from Turkey,” International Journal of Food Properties, vol. 20, no. 11, pp. 2639–2644. https://doi.org/10.1080/10942912.2016.1247100
Monji, F., Tehrani, H., H., Halvaei, Z., and Arbabi B., S., (2011). “Acute and subchronic toxicity assessment of the hydroalcoholic extract of Stachys lavandulifolia in mice,” Acta Med Iran, vol. 49, no. 12, pp. 769–775.
Modarresi, M., Hosseinzadeh, L., Nematy, N., Siavash-Haghighi, Z., M., Ghanbari, K., (2014). “Acute and subchronic toxicological evaluation of Stachys lavandulifolia aqueous extract in Wistar rats”.
Taghikhani, A., Afrough, H., Ansari-Samani, R., Shahinfard, N., Rafieian-Kopaei, M., (2014). “Assessing the toxic effects of hydroalcoholic extract of Stachys lavandulifolia Vahl on rat’s liver,” vol. 115, no. 3. https://doi.org/10.4149/bll_2014_026
Clarke, G., Ting, K., N., Wiart, C., and Fry, J., (2013). “High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates,” Antioxidants, vol. 2(1), pp. 1–10. https://doi.org/10.3390/antiox2010001
Yang, H., Dong, Y., Du, H., Shi, H., Peng, Y., and Li, X., (2011). “Antioxidant compounds from propolis collected in Anhui, China,” Molecules. https://doi.org/10.3390/molecules16043444
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., (1999). “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free radical biology and medicine. https://doi.org/10.1016/S0891-5849(98)00315-3
Chai, T., Mohan, M., Ong, H., Wong, F., (2014). “Antioxidant, iron-chelating and anti-glucosidase activities of Typha domingensis Pers (Typhaceae),” mvol. 13, no. 1, pp. 67–72. DOI: 10.4314/tjpr.v13i1.10
Khanavi M., Hajimahmoodi, M., Cheraghi-Niroomand, M., Kargar, Z., Ajani, Y., Hadjiakhoondi, A., and Oveisi, M. R., (2009). “Comparison of the antioxidant activity and total phenolic contents in some Stachys species,” vol. 8, no. 6, pp. 1143–1147. ISSN 1684–5315
Conforti, F., Menichini, F., Formisano, C., Rigano, D., Senatore, F., Arnold, N. A. and Piozzi, F., (2009). “Comparative chemical composition, free radical-scavenging and cytotoxic properties of essential oils of six Stachys species from different regions of the,” Food Chem. https://doi.org/10.1016/j.foodchem.2009.03.044
Rahimi Khoigani, S., Rajaei, A., and Goli, S., A., H., (2017). “Evaluation of antioxidant activity, total phenolics, total flavonoids and LC–MS/MS characterisation of phenolic constituents in Stachys lavandulifolia,” Taylor & Francis, vol. 31, no. 3, pp. 355–358. https://doi.org/10.1080/14786419.2016.1233410
Guo, H., Saravanakumar, K., and Wang M., H., (2018). “Total phenolic, flavonoid contents and free radical scavenging capacity of extracts from tubers of Stachys affinis,” Biocatal Agric Biotechnol, vol. 15, pp. 235–239. https://doi.org/10.1016/j.bcab.2018.06.009
Kirkan, B., (2019). “Antioxidant potential, enzyme inhibition activity, and phenolic profile of extracts from Stachys cretica subsp. vacillans,” Industrial crops and products. https://doi.org/10.1016/j.indcrop.2019.111639
Yamaç, S., S., (2023). Ereğli/Konya Bölgesindeki Tarım Arazilerinin Evapotranspirasyon Verilerinin Uydu Görüntüleriyle İncelenmesi. Necmettin Erbakan Üniversitesi, Ereğli Tarım Bilimleri Dergisi, 8, 3-1. E-ISSN: 2822-4167
Buharalıoğlu, G. Y., İlhan, R., Yıldırım, H., & Emir, C. Biological Activities of Two Stacys Species: Stachys thirkei K. Koch and Stachys macrantha (K. Kock) Stearn. Erzincan University Journal of Science and Technology, 18(3), 789-798. https://izlik.org/JA67NA62NJ
Güzel, A. (2023). Tüylü Çayın (Stachys lavandulifolia) Fitokimyasal Analizi ve Antioksidan, Antikolinesteraz ve Antiaterojenik Aktivitesi. Journal of the Institute of Science and Technology, 13(4), 2809-2817. https://doi.org/10.21597/jist.1309555
Pashova, S., Karcheva-Bahchevanska, D., Ivanov, K., & Ivanova, S. (2024). Genus Stachys Phytochemistry, traditional medicinal uses, and future perspectives. Molecules, 29(22), 5345. https://doi.org/10.3390/molecules29225345
Ayaz, F., & Eruygur, N. (2022). Investigation of enzyme inhibition potentials, and antioxidative properties of the extracts of endemic Stachys bombycina Boiss. İstanbul Journal of Pharmacy, 52(3), 318-323. https://doi.org/10.26650/IstanbulJPharm.2022.1075293
Aydın, E., Rasgele, P. G., & Dulger, G. (2022). First report on bioaccessibility, antioxidant activity and total phenolic compounds from Stachys thirkei C. Koch using a simulated in vitro digestion system. Journal of Advanced Research in Natural and Applied Sciences, 8(2), 188-200. https://doi.org/10.28979/jarnas.1014968
Aksoy, L., & Demir, S. (2022). Total Phenolic Content, Antioxidant Properties and Element Concentrations of Methanol and Acetone Extracts of Stachys tmolea Boiss. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 22(6), 1271-1278. https://doi.org/10.35414/akufemubid.1099557
Özcan, K., & Acet, T. (2022). Stachys macrantha (K. Koch) Stearn’ın biyolojik aktivitesinin belirlenmesi. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 11(1), 156-163. https://doi.org/10.17798/bitlisfen.1002210

Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyokimyası

Bölüm

Araştırma Makalesi

Yazarlar

Merve Koçak ^*
0000-0001-7590-7190
Türkiye

Yavuz Bağcı
0000-0002-7714-856X
Türkiye

Yayımlanma Tarihi

15 Nisan 2026

Gönderilme Tarihi

12 Ağustos 2025

Kabul Tarihi

6 Ocak 2026

Yayımlandığı Sayı

Yıl 2026 Cilt: 8 Sayı: 1

IZ

https://izlik.org/JA87BN47HL

APA

Koçak, M., & Bağcı, Y. (2026). Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey. Necmettin Erbakan University Journal of Science and Engineering, 8(1), 60-78. https://izlik.org/JA87BN47HL

AMA

1.Koçak M, Bağcı Y. Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey. NEU Fen Muh Bil Der. 2026;8(1):60-78. https://izlik.org/JA87BN47HL

Chicago

Koçak, Merve, ve Yavuz Bağcı. 2026. “Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey”. Necmettin Erbakan University Journal of Science and Engineering 8 (1): 60-78. https://izlik.org/JA87BN47HL.

EndNote

Koçak M, Bağcı Y (01 Nisan 2026) Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey. Necmettin Erbakan University Journal of Science and Engineering 8 1 60–78.

IEEE

[1]M. Koçak ve Y. Bağcı, “Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey”, NEU Fen Muh Bil Der, c. 8, sy 1, ss. 60–78, Nis. 2026, [çevrimiçi]. Erişim adresi: https://izlik.org/JA87BN47HL

ISNAD

Koçak, Merve - Bağcı, Yavuz. “Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey”. Necmettin Erbakan University Journal of Science and Engineering 8/1 (01 Nisan 2026): 60-78. https://izlik.org/JA87BN47HL.

JAMA

1.Koçak M, Bağcı Y. Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey. NEU Fen Muh Bil Der. 2026;8:60–78.

MLA

Koçak, Merve, ve Yavuz Bağcı. “Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey”. Necmettin Erbakan University Journal of Science and Engineering, c. 8, sy 1, Nisan 2026, ss. 60-78, https://izlik.org/JA87BN47HL.

Vancouver

1.Merve Koçak, Yavuz Bağcı. Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey. NEU Fen Muh Bil Der [Internet]. 01 Nisan 2026;8(1):60-78. Erişim adresi: https://izlik.org/JA87BN47HL

Türkiye’ye endemik Stachys gaziantepensis ve Stachys ketenoglui (Lamiaceae) türlerinin antioksidan aktivitesi ve tirozinaz enzim inhibisyon potansiyeli

Öz

Anahtar Kelimeler

Antioxidant activity and tyrosinase enzyme inhibition potential of the endemic Stachys gaziantepensis and Stachys ketenoglui (Lamiaceae) from Turkey

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

IZ

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