Kuraklık Stresine Karşı Bor Bileşiklerinin Soyada (Glycine max. L.) Büyüme Parametrelerine Etkisi

Yıl 2013, , 1 - 13, 22.01.2014

Mahmut Doğan , Aslıhan Avu

https://doi.org/10.17100/nevbiltek.210871

Cited By: 2

Öz

Bu çalışmada materyal olarak soya (Glycine max. L.)
tohumları farklı bor bileşiklerinden oluşan, kontrollü koşullarda
(25±2 °C)
yetiştirilmiştir. Çalışmada sırasıyla; potasyum tetraborat tetrahidrat, amonyum
tetraborat tetrahidratt ve sodyum bor hidrürden 1 mg/l, lityum tetraborat
tetrahidratt ve sodyum tetraborat dekahidrattan 100 mg/l olmak üzere 5 grup belirlenmiştir.
Kullanılan dozlar daha önce yapılan ön çalışmada belirlenmiştir. Soya fideleri
zamana bağlı olarak (kontrol, 3, 6, 9, 12, 15 ve 18 gün) farklı kuraklık uygulamalarına
maruz bırakılarak yapraklarda klorofil, MDA, prolin ve iyon miktarları ölçülmüştür.
Kuraklık stresine karşı bor bileşiklerinin uygulanması amacıyla yapılan
araştırmada, soya bitkisinin bor bileşiklerine karşı tolerans sınırlarının çok
dar olduğu saptanmıştır. Araştırmada stres+potasyumlu bor grubunda, klorofil ve
prolin miktarı artmış, MDA miktarı kontrolle aynı seviyelerde olduğu tespit
edilmiştir. Bu sonuçlara göre potasyum tetraboratın 0.1 mg/l doz uygulamasının
en uygun eşik değeri olduğu ve kuraklığa karşı tüm parametrelerin birbirlerini
destekler nitelikte olduğu ve en iyi sonucu verdiği tespit edilmiştir.

Anahtar Kelimeler

Bor, kuraklık stresi, prolin, soya

The Effects of Boron Compounds on Growth Parameters of Soybean (Glycine max. L.) Under Drought Stress

Öz

In this study, soybean seeds (Glycine
max. L.) were grown under controlled conditions (25±2
°C) composed of different boron compounds. In the study,
5 groups were determined respectively as potassium tetraborate tetrahydrate (1
mg/1), ammonium tetraborate tetrahydrate (1 mg/1), sodium boron hydride (1
mg/1), lithium tetraborate tetrahydrate (100 mg/1), and sodium tetraborate
decahydrate (100 mg/1). The doses used in this study were determined according
to the results of a preliminary study. Soybean seeds were exposed to different
amounts of drought stress based on time (control, 3, 6, 9, 12, 15, and 18
days). Chlorophyll, MDA, amounts of proline and ion in leaf samples were
measured. In this study which was conducted in order to apply boron compounds
against drought stress, it was determined that tolerance limits of soybean
plant for boron compounds is very narrow. In the study, it was revealed that
amounts of chlorophyll and proline increased in stress+potassium boron group
while the amount of MDA was the same as in the control group. According to the
results, it was determined that 0.1mg/l potassium tetraborate application was
the most appropriate threshold level and that all the parameters supported each
other against the drought and yielded the best results.

Anahtar Kelimeler

Boron, drought stress, proline, soybean

Kaynakça

• Ashraf M ., “Breeding for salinity tolerance in plants” Critical Reviews in Plant Sciences 13(1) 17-42 1994.
• Ashraf M. ve Foolad M.R ., “Roles of Glycine Betaine ve Proline in Improving Plant Abiotic Stress Resistance”. Envionmental ve Experimental Botany 59: 206-216 2007.
• Khanna-Chopra R ., Selote DS ., “Acclimation to Drought Stres Generates Oxidative Stress Tolerance in Drought Resistant than-Susceptible Wheat Cultivar Under Field Conditions” Environmental ve Experimental Botany 60: 276–283 2007.
• Mut Z Akay H ve Aydın N ., “Effects of Seed Size ve Drought Stress on Germination ve Seedling Growth of Some at Genotypes (Avena sativa L.)”. African Journal of Agricultural Research 5(10): 1101-1107 2010.
• Aykanat S Semercioğlu T ., Baryt H ., “1999-2007 Yılları arasındaki kurak dönemlerin Ceyhan99 buğdayındaki olumsuz etkileri”. Türkiye VIII. Tarla Bitkileri Kongresi 19-22 Ekim HatayTürkiye 2009.
• Kutlu I. ve Kinaci G ., “Evaluation of Drought Resistance Indicates for Yield ve Its Components in Three Triticale Cultivars” Tekirdağ Ziraat Fakültesi Dergisi 7(2): 95-103 2010.
• Ashraf M. ve Iram A ., “Drought Stress Induced Changes in Some Organic Substances in Nodules ve Other Plant Parts of Two Potential Legumes Differing in Salt Tolerance”. Flora 200: Taban S. and Katkat A.V ., “Effect of salt stress on growth and mineral elements concentrations in shoots and roots of maize plants” Tarım Bilimleri dergisi 6(2): 119-122 2000.
• Bayrak H ., Önder M. Gezgin S ., “Bor Uygulamalarının Nohut (Cicer arietinum L.) Çeşitlerinde Verim ve Bazı Verim Unsurlarına etkileri”. S.Ü. Ziraat Fakültesi Dergisi 19 (35): 66-74 2005. Bowen J.E. ve Gauch H.G ., “Essentiality of Boron for Dryopteris dentata and Selaginella apoda” American Fern Journal 55: 67-73 1965. Soy M ve Güneş A ., Fosforun Domates (Lycopersicon esculentum L.) Bitkisinde Bor Toksisitesini Önlemede Etkisi. Tarım Bilimleri Dergisi 9 (3): 273-277 2003.
• Blevins D.G. and Lukaszewski K .,M ., Boron in Sturucture and Function Annual Rev. Plant Physilogy Plant Molecular Biology 49: 481-500 1998. Punchana S ., Jamjod S. And Rerkasem B ., Response to Boron Toxicity in Boron Efficient and Inefficient Wheat Genotyps 4. Int. Crop Sci. Cong ., Sept. Brisbane Australia. 2004. Hamurcu M ., Harmankaya M ., Soylu S ., Gökmen F ., Gezgin S ., Makarnalık Buğdayın (Triticum durum L.) Bazı Besin Elementleri Kapsamına Farklı Dozlarda Bor ve Demir Uygulamalarının Etkisi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi 20(38): 1-8 200 Choi E ., Kolesik P ., McNeill A ., Collins H ., Zhang Q ., Huynhi B ., Graham R. and Stangoulis J ., The Mechanism of Boron Tolerance for Maintanance of Root Growth in Barley (Hordeum vulgare L.) Plant Cell and Environment 30: 984-993 2007. Nable R.O ., Banuelous S.G. and Paull G.J ., Boron Toxicity Plant and Soil 198:181198 19 Garcia-Gonzalez M ., Mateo P ., Borilla I ., Effects of Boron Deficiency on Photosynthesis and Reductant Sources and Relationship with Nitrogenase Activity in Anabaena PCC 711 Plant Physiol 93: 560- 565 1990.
• Ellis R.H ., Roberts E.H ., Summerfield R.J. ve Cooper J.P ., Environmental control of flowering in barley (Hordeum vulgare L.). II. Rate of development as a function of temperature ve photoperiod ve its modification by low temperature vernelization Annals of Botany 62: 145-158 19 Hoagland D.R. ve Arnon D.I ., The water culture method for growing plants without. Soil. Circ. Calif. Agr. Exp. Sta ., 347-461 1938. Luna C ., Seffino L.G ., Arias C. ve Taleisnik E ., Oxidative stress indicators as selection tools for salt tolerance in Chloris gayana Plant Breeding 119: 341-345 2000. Lutts S ., Kinet J.M. ve Bouharmont J ., NaCl-Induced senesence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance Annals of Botany 78: 389-398 1996.
• Taleisnik E ., Peyrano G ve Arias C ., Response of Chloris gayana cultivars to salinity 1. Germination ve early vegetative growth Trop. Grassl 31: 232-240 1997.
• Bates L. S ., Waldren R.P. ve Teare I.D ., Rapid determination of free proline for water- stres studies. Plant ve Soil 39: 205-207 1973.
• Güneş A. ve Alpaslan M ., Boron uptake ve toxicity in maize genotypes in relation to boron and phosphorus supply. J. Plant Nutr ., 23 (4): 451-550 2000.
• Güneş A ., Ekmeklik (T. aestivum) ve makarnalık (T. durum) buğday genotiplerinin bor alımı üzerine fosforun etkisi. Ankara Üniv. Ziraat Fak. Tarım Bilimleri Dergisi 6 (4): 44-48 2000. Alpaslan M. and Güneş A ., Interactive effects of boron ve salinity stress on the growth membrane permeability ve mineral composition of tomato ve cucumber plants. Plant and Soil 236: 123-128 2001. Murillo-Amador B ., Jones H.G ., Kaya C ., Aguilar R.L ., Garcia-Hernveez J.L ., TroyoDieguez E ., Avila-Serrano N.Y. Rueda-Puente E ., Effects of foliar application of calcium nitrate on growth ve physiologyical attributes of cowpea (Vigna unguicculata L. Walp.) grown under salt stres. Environmental ve Esperimental Botany 58: 188-196 2006. Alfocea F.P ., Estan M.T ., Caro M. and Bolarin M.C ., Response of tomato cultivars to salinity Plant and Soil 150: 203-211 1993. Hernandez J.A. ve Almansa M.S ., Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves. Physiologia Plantarum 115(2): 251–257 2002. Doğan M ., Kılıç H ., Aktan A ., Can E.N ., Tuz stresi altındaki domates (Lycopersicon sp.) fidelerinde kalsiyum miktarı değişimleri. Fırat Üniversitesi Fen Bilimleri Dergisi 21(2): 103– 108 2009.
• Doğan M ., Tıpırdamaz R ., Demir Y ., Salt resistance of tomato species grown in sand culture Plant Soil Environ ., 56(11): 499–507 2010b. Lopez M.V. and Satti S.M.E ., Calcium and potassium-enhanced growth and yield of tomato under sodium chloride stress Plant Science 114: 19-27 1996. Öztürk A ., Kuraklığın Kışlık Buğdayın Gelişmesi ve Verimine Etkisi. Turksh Journal of Agriculture ve Forestry 23: 531-540 1999.
• Kalefetoğlu T. ve Ekmekçi Y ., The effects of drought on plants ve tolerance mechanisms (Review). Gazi Üniversitesi Fen Bilimleri Dergisi 18(4): 723-740 2005.
• Katerji N ., Van-Hoorn J.W ., Hamdy A ., Mastrorilli M ., Response of tomatoes a crop of indeterminate growth to soil salinty. Agricultural water Management 38: 59-68 1998.
• Munne-Bosch S. ve Penuelas J ., Photo-ve Antioxidative Protection During Summer Leaf Senescence in Pistacia lentiscus L. Grown under Mediterranean Field Conditions. Annals of Botany, 92(3): 385-391 2003. Lichtenhaler HK ., Vegetation stress: an introduction to the stress concept in plants. Journal of Plant Physiology 148: 4-14 1996. Islam S ., Malik A.I ., Islam A.K.M.R. and Colmer T.D ., Salt tolerance in a Hordeum marinumTriticum aestivum amphiploid ve its parents Journal of Experimental Botany 58: 1219-1229 200
• Valenzuela A ., The biological significanc of malondialdehtde determination inthe asessment of tissuee oxidative stres. Life Sciences 48: 301-309 1990.
• Hodges D.M ., Delong J.M ., Forney C.F ., Prange R.K ., Improving the thiobarbituric acidreactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin ve other interfering compounds. Planta 207: 604-611 1999.
• Shalata A. ve Tal M ., The effect of salt stress on lipid peroxidation ve antioxidants in the leaf of the cultivated tomato ve its wild salt-tolerant relative Lycopersicon pennellii Physiol Plant 104: 169-174 1998.
• Arıbaykal Ş. ve Öncel I ., Buğday Fidelerinin Bor Toksisitesine Toleransında Çözünür Fenolik ve Çözünür Protein Miktarındaki Değişimler. Cumhuriyet Üniversitesi Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi 27(1): 13-25 2006.
• Başalp A ., Öncel I ., Koç E ., Bor (B) Toksisitesine Toleranslı ve Duyarlı Buğday Fidelerinde Bazı Fizyolojik ve Biyokimyasal Değişimlerin Belirlenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 15(3): 135-141 2011.
• Knörzer O.C ., Lederer B ., Durner J ., Böger P ., Antioxidative defense activation in soybean cells. Physiologia Plantarum 107: 294-302 1999.
• Cherki G ., Foursy A. ve Fares K ., Effects of salt on growth inorganic ions ve proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Enviromental and Experimental Botany 47: 39-50 2002. Stefl M. Tracka I. ve Vratny P ., Proline biosynthesis in winterplants due to exposure to low temperatures. Biol. Plant ., 20: 119-128 1978. Kushad M.M. ve Yelenkosky G. Evaluation of polyamine ve proline levels during low temperature acclimation of citrus. Plant physiol. 84: 692-695 1987.
• Caines A.M. and Shennan C ., Interacti ve effects of Ca +2 and differing in Ca +2 use efficiency Plant Physiol Biochem ., 37(7/8): 569-576,1999.
• Bondada B.R ., Syvertsen J.P. ve Albrigo L.G ., Urea nitrogen uptake by citrus leaves. Hort. Science 36: 1061–1066 2001.
• Yağmur M ., Kaydan D ., Okut N ., Potasyum Uygulamasının Tuz Stresindeki Arpanın Fotosentetik Pigment İçeriği Ozmotik Potansiyel K + /Na + Oranı ile Bitki Büyümesindeki Etkileri. Ankara Üniversitesi Ziraat Fakültesi Tarım Bilimleri Dergisi 12 (2): 188-194 2006.
• Din C ., S. M. Mehdi M. Sarfraz Hassan Ghulam and M. Sadiq Comparative efficiency of foliar ve soil application of K on salt tolerance in rice. Pakistan Journal of Biological Sciences 4: 815-817 2001.
• Shirazi M.U ., M. Y. Ashraf M. A. Khan ve M. H. Naqvi. Potassium induced salinity tolerance in wheat (Triticum aestivum L.). Int. J. Environ. Sci. Tech ., 2: 233-236 2005.
• Marschner H. Mineral nutritions of higher plants. Academic Pres. London GB. 1995.
• Embleton T.W. and Jones W.W.. Foliar-Applied nitrogen for citrus fertilization. J. Environ. Quality. 3 (4): 388-391 1974.
• Chapman H.D ., The mineral nutrition of citrus. Plant Physiol. 17: 333-335 1976.
• Çakmak I ., Römheld V ., Boron Deficiency-Induced Impairments of Cellular Functions in Plants Plant ve Soil 193: 71-83 1997. Ben Amor N. Jimenez A ., Megdiche W ., Lundqvist M ., sevilla F ., Abdelly C ., Response of antioxidant systems to NaCl stres in the halophyte Cakile maritima. Physiologia Plantarum 126: Yağmur B ., Farklı Seviyelerde Uygulanan Potasyumun Anasonun Verim ve Yaprak Besin Maddesi İçeriğine Etkisi. Ege Üniversitesi Ziraat Fakültesi Dergisi 46 (1): 17-24 2009.
• Alıcı Y. ve Öncel I ., Buğdayda Bor Toksisitesi ile Fosfor Arasındaki Etkileşimin Büyüme ve Çözünür Karbonhidratlar ile ilişkisinin incelenmesi. Cumhuriyet Üniversitesi Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi 29(1): 29-46 2008.