Lasiocampa trifolii (Denis & Schiffermüller, 1775)’nin Dahili Bakteriyel Çeşitliliği: Yeni Bir Olası Okibacterium sp.

Yıl 2021, Cilt: 10 Sayı: 1, 44 - 55, 01.06.2021

Ali Sevim , Elif Sevim

Öz

Böceklerin bağırsak sistemlerinde bulunan mikroorganizmaların bu böceklerin sindirim ve beslenme süreçlerinde faydalı olduğu bilinmektedir. Bu anlamda, böceklerin bağırsak sistemindeki bakterilerin tanımlanması, özellikle ekonomik olarak zararlı veya faydalı böcek türlerinin besleyici ve sindirim özelliklerinin belirlenmesi açısından özellikle önemlidir. Bu çalışmada, Lasiocampa trifolii'nin (Denis & Schiffermüller, 1775) (Lepidoptera: Lasiocampidae) dahili bakteriyel florası kültür bağımlı teknikler kullanarak belirlenmiştir. Bu böceğin larvaları, çeşitli otlar ve çalılar, meşe, kavak ve süpürge çimi gibi bitki yapraklarıyla beslenmektedir. Larvalar Mayıs-Haziran (2019) aylarında Türkiye'nin Kırşehir bölgesinden toplanmış ve bakteri izolasyonu için laboratuvara getirilmiştir. Dört adet 3-4. instar larvalar bakteri izolasyonu için kullanılmıştır. İzole edilen bakteriler, 16S rRNA dizin analizi kullanılarak tanımlanmıştır. On bir bakteri izole edilmiş ve bunlar Bacillus sp. TT1, Micrococcus luteus TT2, Arthrobacter sp. TT3, Corynebacterium sp. TT4, Arthrobacter agilis TT5, Micrococcus luteus TT6, Micrococcus luteus TT7, Arthrobacter sp. TT8, Okibacterium sp. TT9, Staphylococcus haemolyticus TT10 ve Bacillus sp. TT11 olarak tanımlanmıştır. İzolat TT9, Okibacterium cinsi içinde yer alan yeni bir tür gibi görünmekte ve bu bakteri bu çalışma ile şimdiye kadar herhangi bir hayvandan ilk kez izole edilmiştir. Elde edilen sonuçlar beslenme ve böcek hastalıkları açısından tartışılmıştır.

Anahtar Kelimeler

Bakteri, böcekler, simbiyoz, 16S rRNA, Okibacterium

Destekleyen Kurum

Kırşehir Ahi Evran Üniversitesi, BAP birimi

Proje Numarası

ZRT.A4.19.023

Kaynakça

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