Çinko Nanopartiküllerinin (ZnO NP) Biyosentezi, Karakterizasyonu ve Anti- Bakteriyel Etkisinin İncelenmesi

Yıl 2021, Cilt: 10 Sayı: 1, 56 - 66, 01.06.2021

Kevser Günay Zeliha Leblebici Fatih Doğan Koca

Öz

Bitki özütleri ile sentezlenen metal nanopartiküllerin diğer sentez yöntemlerine kıyasla üretimi kolay, güvenilir ve ucuz bir yöntemdir. Nasturtium officinale (su teresi) ağır metalleri akümüle etme yeteneğine sahiptir. Bu çalışmada, Nasturtium officinale özütü kullanılarak ZnO nanopartiküllerinin biyosentezi, karakterizasyonu ve antibakteriyel etkisi incelenmiştir. Nanopartiküllerin karakterizasyonu X-ışını analizi (XRD), Fourier dönüşümlü kızılötesi spektroskobi analizi (FT-IR), Yüzey yükü ölçümü (ZETA), Taramalı elektron mikroskobu (SEM) yöntemi ile gerçekleştirilmiştir. Nasturtium officinale özütü ile biyosentezi gerçekleştirilen ZnO NP’nin yüzey yükü -14 mV’de belirlenmiştir. ZnO NP’nin Taramalı elektron mikroskobu ile parçacık boyutu ortalama 90 nm olarak bulunmuştur. ZnO nanopartiküllerinin Vibrio parahaemolyticus ve Aeromonas hydrophile bakterileri üzerindeki antibakteriyel aktiviteleri, minimal inhibe edici konsantrasyon (MİK) yöntemiyle ölçülmüştür. Antibakteriyel aktivite ölçümlerinde, sentezlenen nanopartiküllerin V. parahaemolyticus ve A. hydrophile bakteriyel aktivite inhibisyonu sağladığı tespit edilmiştir.

Anahtar Kelimeler

Çinko Nanopartikülü (ZnO NP), , Biyosentez,, Nasturtium officinale, SEM, Anti-Bakteriyel Etki

Destekleyen Kurum

Nevşehir Hacıbektaş Veli Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

ABAP-20F41 ve GAP20F1 projeleri

Teşekkür

Bu çalışma Nevşehir Hacıbektaş Veli Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi ABAP-20F41 ve GAP20F1 nolu projeleri tarafından destek almıştır. NEÜ BAP birimine desteklerinden dolayı teşekkür ederiz.

Kaynakça

• Arıhan S.K., “Antik Dönemde Bitkisel Tıp ve Tedavi” Ankara Üniversitesi, Sosyal Bilimler Enstitüsü, Yüksek Lisans Tezi, 199s, Ankara, 2003.
• Arıhan S.K., “Antik Dönemde Bitkisel Tıp ve Tedavi” Ankara Üniversitesi, Sosyal Bilimler Enstitüsü, Yüksek Lisans Tezi, 199s, Ankara, 2003.
• Ceran B., “Antik Mısır ve Anadolu Uygarlıklarında Tıp” Selçuk Üniversitesi, Sosyal Bilimler Enstitüsü, Yüksek Lisans Tezi, 38s, Konya, 2008.
• Ceran B., “Antik Mısır ve Anadolu Uygarlıklarında Tıp” Selçuk Üniversitesi, Sosyal Bilimler Enstitüsü, Yüksek Lisans Tezi, 38s, Konya, 2008.
• Albrecht M.A., Evan C.W., Raston C.R., “Green chemistry and the health implications of nanoparticles” Green Chemistry, 8, 417-32, 2006
• Albrecht M.A., Evan C.W., Raston C.R., “Green chemistry and the health implications of nanoparticles” Green Chemistry, 8, 417-32, 2006
• Osuwa J.C., Anusionwu P.C., “Some advances and prospects in nanotechnology: a review” Asian Journal of Information Technology, 10, 96-100, 2011
• Osuwa J.C., Anusionwu P.C., “Some advances and prospects in nanotechnology: a review” Asian Journal of Information Technology, 10, 96-100, 2011
• Buzea C., Pacheco I., Robbie K., “Nanomaterials and nanoparticles: sources and toxicity” Biointerphases, 2 (4), 17-71, 2007
• Buzea C., Pacheco I., Robbie K., “Nanomaterials and nanoparticles: sources and toxicity” Biointerphases, 2 (4), 17-71, 2007
• Santhoskumar J., Venkat Kumar S., Rajeshkumar S,. “Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen” Resource-Efficient Technologies, 3(4), 459-65, 2017
• Santhoskumar J., Venkat Kumar S., Rajeshkumar S,. “Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen” Resource-Efficient Technologies, 3(4), 459-65, 2017
• Chen J., Liu X., Wang C., Yin S.S., Li X.L., Hu W.J., “Nitric oxide ameliorates zinc oxide nanoparticles-induced phytotoxicity in rice seedlings” Journal of Hazardous Materials, 297, 173-82, 2016
• Chen J., Liu X., Wang C., Yin S.S., Li X.L., Hu W.J., “Nitric oxide ameliorates zinc oxide nanoparticles-induced phytotoxicity in rice seedlings” Journal of Hazardous Materials, 297, 173-82, 2016
• Nair S., Sasidharan A., Divya Rani V.V., Menon D., Nair S., Manzoor K., “Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells” Journal of Materials Science: Materials in Medicine, 20, 235-41, 2009
• Nair S., Sasidharan A., Divya Rani V.V., Menon D., Nair S., Manzoor K., “Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells” Journal of Materials Science: Materials in Medicine, 20, 235-41, 2009
• Premanathan M., Karthikeyan K., Jeyasubramanian K., Manivannan G., “Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation” Nanomedicine, 7(2), 184-92, 2011
• Premanathan M., Karthikeyan K., Jeyasubramanian K., Manivannan G., “Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation” Nanomedicine, 7(2), 184-92, 2011
• Hanley C., Layne J., Punnoose A., Reddy K.M., Coombs I., Coombs A., “Perferential killing of cancer cells anda activated human T cells using ZnO nanoparticles” Nanotecnology, 19(29), 295-303, 2008
• Hanley C., Layne J., Punnoose A., Reddy K.M., Coombs I., Coombs A., “Perferential killing of cancer cells anda activated human T cells using ZnO nanoparticles” Nanotecnology, 19(29), 295-303, 2008
• Agarval H., Venkat Kumar S., Rajeshkumar S., “A review on green synthesis of zinc oxide nanoparticles - An eco-friendly approach” Resource-Efficient Tecnologies, 3(4), 406-413, 2017
• Agarval H., Venkat Kumar S., Rajeshkumar S., “A review on green synthesis of zinc oxide nanoparticles - An eco-friendly approach” Resource-Efficient Tecnologies, 3(4), 406-413, 2017
• Yuvakkumar R., Suresh J., Nathanael A.J., Sundrarajan M., Hong S.I., “Novel green synthetic strategy to prepare ZnO nanocrystals using rambutan (Nephelium lappaceum L.) peel extract and its antibacterial applications” Materials Science and Engineering, C, Materials for Biological Applications, 41, 17-27, 2014
• Yuvakkumar R., Suresh J., Nathanael A.J., Sundrarajan M., Hong S.I., “Novel green synthetic strategy to prepare ZnO nanocrystals using rambutan (Nephelium lappaceum L.) peel extract and its antibacterial applications” Materials Science and Engineering, C, Materials for Biological Applications, 41, 17-27, 2014
• Akhtar M., Panwar J., Yun Y.S., “Biogenic synthesis of metallic nanoparticles by plant extracts” ACS Sustainable Chemistry and Engineering, 1, 591-602, 2013 Kumar V., Yadav S.K., “Plant mediated synthesis of silver and gold nanoparticles and their applications” Journal of Chemical Technology and Biotechnology, 84(2), 151-157, 2009
• Akhtar M., Panwar J., Yun Y.S., “Biogenic synthesis of metallic nanoparticles by plant extracts” ACS Sustainable Chemistry and Engineering, 1, 591-602, 2013 Kumar V., Yadav S.K., “Plant mediated synthesis of silver and gold nanoparticles and their applications” Journal of Chemical Technology and Biotechnology, 84(2), 151-157, 2009
• Rose P., Faulkner K., Williamson G., Mithen R., “7-Methylsulfinylheptyl and 8- methylsulfinyloctyl isothiocyanates from watercress are potent inducers of phase II enzymes” Carcinogenesis, 21(11), 1983-1988, 2000
• Rose P., Faulkner K., Williamson G., Mithen R., “7-Methylsulfinylheptyl and 8- methylsulfinyloctyl isothiocyanates from watercress are potent inducers of phase II enzymes” Carcinogenesis, 21(11), 1983-1988, 2000
• Lee J.S., Newman M.E., “Aquaculture-an introduction. Agriscience and technology series” Interstate Publishers, 445-446, Illinois, 1997
• Lee J.S., Newman M.E., “Aquaculture-an introduction. Agriscience and technology series” Interstate Publishers, 445-446, Illinois, 1997
• Stern K.R., “Introductory Plant Biology” Wm. C. Brown Publishers, England, 521s, 1994
• Stern K.R., “Introductory Plant Biology” Wm. C. Brown Publishers, England, 521s, 1994
• Duke J.A., “Handbook of phytochemical constituents of grass herbs and other economic plants” Boca Raton, CRC Pres. FL. 33431, 654pp, 1992
• Duke J.A., “Handbook of phytochemical constituents of grass herbs and other economic plants” Boca Raton, CRC Pres. FL. 33431, 654pp, 1992
• Shaheen I., Ahmad K.S.,“Chromatographic identification of “green capping agents” extracted from Nasturtium officinale (Brassicaceae) leaves for the synthesis of MoO3 nanoparticles” Journal of Seperation Science, 43 (3), 598- 605, 2020
• Shaheen I., Ahmad K.S.,“Chromatographic identification of “green capping agents” extracted from Nasturtium officinale (Brassicaceae) leaves for the synthesis of MoO3 nanoparticles” Journal of Seperation Science, 43 (3), 598- 605, 2020
• Bayrami A., Ghorbani E., Rahim S., Yangjeh P., Bayrami A.K.M., “Enriched zinc oxide nanoparticles by Nasturtium officinale leaf extract: Joint ultrasound-microwave-facilitated synthesis, characterization, and implementation for diabetes control and bacterial inhibition” Ultrasonics Sonochemistry, 58, 104613, 2019
• Bayrami A., Ghorbani E., Rahim S., Yangjeh P., Bayrami A.K.M., “Enriched zinc oxide nanoparticles by Nasturtium officinale leaf extract: Joint ultrasound-microwave-facilitated synthesis, characterization, and implementation for diabetes control and bacterial inhibition” Ultrasonics Sonochemistry, 58, 104613, 2019
• Gunalan S., Sivaraj R., Rajendran V., “Green synthesized ZnO nanoparticles against bacterial and fungal pathogens” Progress in Natural Science: Materials International, 22 (6), 693-700, 2012
• Gunalan S., Sivaraj R., Rajendran V., “Green synthesized ZnO nanoparticles against bacterial and fungal pathogens” Progress in Natural Science: Materials International, 22 (6), 693-700, 2012
• Ildiz N., Baldemir A., Altinkaynak C., Özdemir N., Yilmaz V., Ocsoy I., “Self Assembled Snowball-Like Hybrid Nanostructures Comprising Viburnum opulus L. Extract and Metal Ions for Antimicrobial and Catalytic Applications” Enzyme and Microbial Technology, 102, 60-66, 2017
• Ildiz N., Baldemir A., Altinkaynak C., Özdemir N., Yilmaz V., Ocsoy I., “Self Assembled Snowball-Like Hybrid Nanostructures Comprising Viburnum opulus L. Extract and Metal Ions for Antimicrobial and Catalytic Applications” Enzyme and Microbial Technology, 102, 60-66, 2017
• Lu W., Qin X., Liu S., Chang G., Zhang Y., Luo Y., “Economical, green synthesis of fluorescent carbon nanoparticles and their use as probes for sensitive and selective detection of mercury(II) ions” Analytical Chemistry, 84(12), 5351-7, 2012
• Lu W., Qin X., Liu S., Chang G., Zhang Y., Luo Y., “Economical, green synthesis of fluorescent carbon nanoparticles and their use as probes for sensitive and selective detection of mercury(II) ions” Analytical Chemistry, 84(12), 5351-7, 2012
• Khatami M., Varma R.S., Zafarnia N., Yaghoobi H., Sarani M., Kumar V.G.. “Applications of green synthesized Ag, ZnO and Ag/ZnO nanoparticles for making clinical” Sustainable Chemistry and Pharmacy, 10, 9-15, 2018
• Khatami M., Varma R.S., Zafarnia N., Yaghoobi H., Sarani M., Kumar V.G.. “Applications of green synthesized Ag, ZnO and Ag/ZnO nanoparticles for making clinical” Sustainable Chemistry and Pharmacy, 10, 9-15, 2018
• Edelstein A.D., Cammarata R.C., “Nanomaterials Synthesis Properties and Applications” Taylor and Francis, Boca Raton, Fla, USA, 1996.
• Edelstein A.D., Cammarata R.C., “Nanomaterials Synthesis Properties and Applications” Taylor and Francis, Boca Raton, Fla, USA, 1996.
• Sudip S., Kumar S.I., Mandal A., Aslan T., Ustun Y., Yılmaz E.Ş., Katı A., Demirbas A., Mandal A.K., and Ocsoy I., “Biosynthesis of silver nanoparticles and their versatile antimicrobial properties” Materials Research Express, 6, 1, 2019
• Sudip S., Kumar S.I., Mandal A., Aslan T., Ustun Y., Yılmaz E.Ş., Katı A., Demirbas A., Mandal A.K., and Ocsoy I., “Biosynthesis of silver nanoparticles and their versatile antimicrobial properties” Materials Research Express, 6, 1, 2019
• Demirbas A., Kislakci E., Karaagac Z., Onal I., Ildız N., Öçsoy İ., “Preparation of biocompatible and stable iron oxide nanoparticles using anthocyanin integrated hydrothermal method and their antimicrobial and antioxidant properties” Materıals Research Express, 6, 12, 2019
• Demirbas A., Kislakci E., Karaagac Z., Onal I., Ildız N., Öçsoy İ., “Preparation of biocompatible and stable iron oxide nanoparticles using anthocyanin integrated hydrothermal method and their antimicrobial and antioxidant properties” Materıals Research Express, 6, 12, 2019
• Demirbas A., Yılmaz V., Ildız N., Baldemir A., Öçsoy İ., “Anthocyanins-rich berry extracts directed formation of Ag NPs with the investigation of their antioxidant and antimicrobial activities” Journal of Molecular Lıquıds, 248, 1044-1049, 2017
• Demirbas A., Yılmaz V., Ildız N., Baldemir A., Öçsoy İ., “Anthocyanins-rich berry extracts directed formation of Ag NPs with the investigation of their antioxidant and antimicrobial activities” Journal of Molecular Lıquıds, 248, 1044-1049, 2017
• Ceylan R., Demirbaş A., Öçsoy İ., Aktumsek A., “Green synthesis of silver nanoparticles using aqueous extracts of three Sideritis species from Turkey and evaluations bioactivity potentials” Sustaınable Chemıstry and Pharmacy, 21, 10, 2021
• Ceylan R., Demirbaş A., Öçsoy İ., Aktumsek A., “Green synthesis of silver nanoparticles using aqueous extracts of three Sideritis species from Turkey and evaluations bioactivity potentials” Sustaınable Chemıstry and Pharmacy, 21, 10, 2021
• Dadi S., Celik C., Ocsoy I., “Gallic acid nanoflower immobilized membrane with peroxidase-like activity for m-cresol detection” Scientific Reports, 10,1, 2020
• Dadi S., Celik C., Ocsoy I., “Gallic acid nanoflower immobilized membrane with peroxidase-like activity for m-cresol detection” Scientific Reports, 10,1, 2020
• Sastry M., Mayya K.S., Bandyopadhyay K., “pH Dependent changes in the optical properties of carboxylic acid derivatized silver colloidal particles” Colloids and Surfaces A, 127(1-3), 221-8, 1997
• Sastry M., Mayya K.S., Bandyopadhyay K., “pH Dependent changes in the optical properties of carboxylic acid derivatized silver colloidal particles” Colloids and Surfaces A, 127(1-3), 221-8, 1997
• Kalimuthu K., Babu R.S., Venkataraman D., Bilal M., Gurunathan S., “Biosynthesis of silver nanocrystals by Bacillus licheniformis” Colloids and Surfaces B, 65(1), 150-3, 2008
• Kalimuthu K., Babu R.S., Venkataraman D., Bilal M., Gurunathan S., “Biosynthesis of silver nanocrystals by Bacillus licheniformis” Colloids and Surfaces B, 65(1), 150-3, 2008
• Varghese E., George M., “Green synthesis of zinc oxide nanoparticles” International Journal of Advance Research in Science and Engineering, 4(1), 307- 14, 2015
• Varghese E., George M., “Green synthesis of zinc oxide nanoparticles” International Journal of Advance Research in Science and Engineering, 4(1), 307- 14, 2015
• Yedurkar S., Maurya C., Mahanwar P., “Biosynthesis of zinc oxide nanoparticles using ixora coccinea leaf extract-A green approach” Journal of Synthesis Theory and Applications, 5(1), 1-14, 2016
• Yedurkar S., Maurya C., Mahanwar P., “Biosynthesis of zinc oxide nanoparticles using ixora coccinea leaf extract-A green approach” Journal of Synthesis Theory and Applications, 5(1), 1-14, 2016
• Rizvi S.A.A., Saleh A.M., “Applications of nanoparticle systems in drug delivery technology” The Saudi Pharmaceutical Journal 26(1), 64-70, 2018
• Rizvi S.A.A., Saleh A.M., “Applications of nanoparticle systems in drug delivery technology” The Saudi Pharmaceutical Journal 26(1), 64-70, 2018
• Etape E.P., Foba-Tendo J., Ngolui L.J., Namondo B.V., Yollande F.C., Nguimezong, M.B.N., “Structural Characterization and Magnetic Properties of Undoped and Ti-Doped ZnO Nanoparticles Prepared by Modified Oxalate Route” Journal of Nanomaterials, 9072325, 2018.
• Etape E.P., Foba-Tendo J., Ngolui L.J., Namondo B.V., Yollande F.C., Nguimezong, M.B.N., “Structural Characterization and Magnetic Properties of Undoped and Ti-Doped ZnO Nanoparticles Prepared by Modified Oxalate Route” Journal of Nanomaterials, 9072325, 2018.
• Fu L., Fu Z., “Plectranthus amboinicus leaf extract-assisted biosynthesis of ZnO nanoparticles and their photocatalytic activity” Ceramics International, 41 (2), 2492-2496, 2015
• Fu L., Fu Z., “Plectranthus amboinicus leaf extract-assisted biosynthesis of ZnO nanoparticles and their photocatalytic activity” Ceramics International, 41 (2), 2492-2496, 2015
• Rajiv P., Rajeshwari S., Vencatesh R., “Bio-Fabrication of zinc oxide nanoparticles using leaf extract of Parthenium hysterophorus L. and its size-dependent antifungal activity against plant fungal pathogens” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 112, 384-387, 2013
• Rajiv P., Rajeshwari S., Vencatesh R., “Bio-Fabrication of zinc oxide nanoparticles using leaf extract of Parthenium hysterophorus L. and its size-dependent antifungal activity against plant fungal pathogens” Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 112, 384-387, 2013
• Minha Naseer M., Aslam U., Bushra Khalid B., Chen B., “Green route to synthesize Zinc Oxide Nanoparticles using leaf extracts of Cassia fstula and Melia azadarach and their antibacterial potential” Scıentıfıc Reports, 10, 9055, 2020
• Minha Naseer M., Aslam U., Bushra Khalid B., Chen B., “Green route to synthesize Zinc Oxide Nanoparticles using leaf extracts of Cassia fstula and Melia azadarach and their antibacterial potential” Scıentıfıc Reports, 10, 9055, 2020
• Pourjavadi A., Soleyman R., “Silver nanoparticles with gelatin nanoshells: photochemical facile green synthesis and their antimicrobial activity” The Journal of Nanoparticle Research, 13, 4647–4658, 2011
• Pourjavadi A., Soleyman R., “Silver nanoparticles with gelatin nanoshells: photochemical facile green synthesis and their antimicrobial activity” The Journal of Nanoparticle Research, 13, 4647–4658, 2011
• Zahra Obeızı Z., Benbouzid H., Sihem O., Bououdina M., “Biosynthesis of Zinc oxide nanoparticles from essential oil of Eucalyptus globulus with antimicrobial and anti-biofilm activities” Materials Today Communications 25, 101553, 2020
• Zahra Obeızı Z., Benbouzid H., Sihem O., Bououdina M., “Biosynthesis of Zinc oxide nanoparticles from essential oil of Eucalyptus globulus with antimicrobial and anti-biofilm activities” Materials Today Communications 25, 101553, 2020
• Jayappa M.D., Ramaiah C.K., Kumar M.A.P. “Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro grown callus of Mussaenda frondosa L.: characterization and their applications” Applied Nanoscience, 10, 3057–3074, 2020
• Jayappa M.D., Ramaiah C.K., Kumar M.A.P. “Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro grown callus of Mussaenda frondosa L.: characterization and their applications” Applied Nanoscience, 10, 3057–3074, 2020
• Gomathi R., Suhana H., “Green synthesis, characterization and antimicrobial activity of zinc oxide nanoparticles using Artemisia pallens plant extract” Inorganic and Nano-Metal Chemistry, 12, 04, 2020
• Gomathi R., Suhana H., “Green synthesis, characterization and antimicrobial activity of zinc oxide nanoparticles using Artemisia pallens plant extract” Inorganic and Nano-Metal Chemistry, 12, 04, 2020
• Taghizadeh S.M., Lal N., Ebrahiminezhad A., Moeini F., Seifan, M., Ghasemi Y., Berenjian A., “Green and Economic Fabrication of Zinc Oxide (ZnO) Nanorods as a Broadband UV Blocker and Antimicrobial Agent” Nanomaterials, 10(3), 530, 2020
• Taghizadeh S.M., Lal N., Ebrahiminezhad A., Moeini F., Seifan, M., Ghasemi Y., Berenjian A., “Green and Economic Fabrication of Zinc Oxide (ZnO) Nanorods as a Broadband UV Blocker and Antimicrobial Agent” Nanomaterials, 10(3), 530, 2020
• Ahmad T., Bustam A.M., Zulfiqar M., Moniruzzaman M., Idris A., Igbal J., Asghar H.M.A., Ullah S., “Controllable phytosynthesis of gold nanoparticles and investigation of their size and morphology-dependent photocatalytic activity under visible light” Journal of Photochemistry and Photobiology A: Chemistry, 392, 112429, 2020
• Ahmad T., Bustam A.M., Zulfiqar M., Moniruzzaman M., Idris A., Igbal J., Asghar H.M.A., Ullah S., “Controllable phytosynthesis of gold nanoparticles and investigation of their size and morphology-dependent photocatalytic activity under visible light” Journal of Photochemistry and Photobiology A: Chemistry, 392, 112429, 2020
• Pillai A.M., Sivasankarapillai V.S., Rahdar A., Joseph J., Sadeghfar F., Anuf R., Rajesh A.K., Kyzas G.Z., “Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity” Journal of Molecular Structure, 1211, 128107, 2020
• Pillai A.M., Sivasankarapillai V.S., Rahdar A., Joseph J., Sadeghfar F., Anuf R., Rajesh A.K., Kyzas G.Z., “Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity” Journal of Molecular Structure, 1211, 128107, 2020