SERS Characterization of Lysozyme Aptasensor Prepared for Latent Fingerprint Visualization on Surfaces

Yıl 2024, Cilt: 13 Sayı: 2, 26 - 33, 31.12.2024

Deniz Saatoğlu , Aybuke A. Isbır Turan , Zafer Üstündağ , Mustafa Oğuzhan Çağlayan , Işık Perçin Demirçelik

https://doi.org/10.17100/nevbiltek.1597365

Öz

Parmak izleri, suç mahalli soruşturmalarında kurbanları ve failleri tespit etmede önemli bir rol oynayan önemli kanıtlardır. Bu çalışma, potansiyel şüphelilerin güvenli bir şekilde tespitini iyileştirmek için nanopartiküller ve aptasensör tabanlı bir biyosensör kullanarak gizli parmak izlerinin görselleştirilmesini geliştirmeye odaklanmaktadır. Bu araştırmada, parmak izi görüntüleme için altın nanopartiküller (AuNP'ler) sentezlenerek ve 5,5'-Dithiobis-2-nitrobenzoik asit (DTNB) ve bir lizozim aptameri ile modifiye edilmiştir. Altın nanopartikül yüzeyi modifiye edildikten sonra, çözelti parmak izi içeren silikon yüzeylere uygulanmıştır. Parmak izlerindeki lizozim üzerindeki aptamer tabanlı Raman etiketinin immobilize edilmesinden sonra alınan SERS ölçümleri, 1337 cm⁻¹'de DTNB'deki nitro grubunun simetrik gerilmesine ait bir pik gözlemlenmiştir.

Anahtar Kelimeler

Forensic Sciences, Latent Fingerprint, Lysozyme Apasensor

Kaynakça

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