Bitki doku kültürlerinde ince hücre tabaka (TCL) kültür sistemi

Öz

Bitki ıslahı ve yetiştiriciliği açısından büyük öneme sahip olan bitki doku kültürü teknikleri, özellikle in vitro koşullarda bitkisel üretim için çeşitli yöntemleri kapsayan geniş bir bilimsel alandır. In vitro koşullarda bitki üretiminde kullanılan tekniklerin başarısını etkileyen en önemli faktörlerden biri eksplant tipinin belirlenmesidir. İnce hücre tabaka (TCL) kültür sistemi ilk olarak 1973 yılında Tran Thanh Van tarafından ortaya atılmıştır. TCL kültür sistemi, ince hücre ve doku katmanlarını içeren uzunlamasına ya da enine kesilmiş eksplantların kültüre alınmasıyla, bu eksplantlardan doku, organ, embriyo veya tam bitki gibi yapıların rejenerasyonunun sağlandığı bir bitki doku kültürü tekniğidir. Bu teknik, in vitro mikroçoğaltım, sentetik tohum üretimi, kriyoprezervasyon ve genetik çalışmalarda başarı ile kullanılmaktadır. Bitki büyüme düzeltme faktörü ve geometrik faktör kavramları, bir TCL eksplantının rejenerasyon potansiyelinin geleneksel bir eksplantınkinden daha büyük olduğunu göstermiştir.

Anahtar Kelimeler

• İnce Hücre Tabaka
• TCL
• in vitro
• rejenerasyon
• eksplant

Thin cell layer (TCL) culture system in plant tissue cultures

Öz

Plant tissue culture techniques, which are of great importance in terms of plant breeding and cultivation, are a wide scientific area that includes various methods for plant production especially in in vitro conditions. One of the most important factors affecting the success of the techniques used in plant production in in vitro conditions is determination of explant type. Thin cell layer (TCL) culture system was first described by Tran Thanh Van in 1973. TCL culture system is a plant tissue culture technique in which structures such as tissue, organs, embryos, or whole plants are regenerated by culturing longitudinally or transversely excised explants containing thin layers of cells and tissue. This technique has been successfully used in in vitro micropropagation, synthetic seed production, cryopreservation and genetic studies. The concepts of plant growth correction factor and geometric factor have shown that the regeneration potential of a TCL explant is greater than that of a conventional explant.

Anahtar Kelimeler

• Thin cell layer
• TCL
• in vitro
• regeneration
• explant

Kaynakça

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