SYNTHESIS AND ANIMICROBIAL ACTIVITY OF WATER-SOLUBLE SULFONATO CALIX[8]AREN BINDING WITH DNA

Öz

   In this study; it was synthesized a water soluble calixarene derivative so-called as versatile molecules for the design and synthesis of receptors having mimic properties or affect biological functions. The structure of the synthesized sulfonato calix[8]arene (sCLX8) derivative was clarified by spectroscopic methods such as FT-IR and ¹H-NMR. Antibacterial activity of the sulfonate calix[8]arene molecule was investigated by disk diffusion test on gram-positive and gram-negative bacterial lines such as B. subtilis and E. coli, respectively. Furthermore, possible interaction between the double-stranded DNA (dsDNA) and calixarene was studied by agarose gel electrophoresis and UV-Vis spectrophotometry in order to see the binding behavior of the functionalized water-soluble calixarene skeleton toward macromolecules.

Anahtar Kelimeler

• Sulphonato calix,8,aren,DNA,Disc diffusion,B. Subtilis,E. Coli

DNA İLE BAĞLANABİLEN SUDA ÇÖZÜNÜR SÜLFONATO KALİKS[8]AREN SENTEZİ VE ANTİMİKROBİYAL AKTİVİTESİ

Öz

   Bu çalışmada; biyolojik fonksiyonları taklit edebilen veya etkileyen reseptörlerin tasarımı ve sentezi için çok yönlü moleküller olarak bilinen kaliksarenlerin suda çözünür bir türevi sentezlenmiştir. Sentezlenen sülfonato kaliks[8]aren (sCLX8) türevinin yapısı FT-IR ve ¹H-NMR gibi spektroskopik yöntemlerle aydınlatılmıştır. Yapısı aydınlatılan sülfonato kaliks[8]aren molekülünün B.subtilis gibi gram pozitif ve E.coli gibi gram negatif özellikteki bakteri hatları üzerine disk difüzyon testi ile antibakteriyel aktivitesi incelenmiştir. Yine fonksiyonel hale getirilmiş suda çözünür kaliksaren iskeletinin, makromoleküllere karşı bağlanma davranışını görebilmek için çift zincirli DNA (dsDNA) ile olan muhtemel etkileşimi agaroz jel elektroforezi ve UV-Vis spektrofotometresi ile çalışılmıştır.

Anahtar Kelimeler

• Sülfonato kaliks,8,aren,DNA,Disk difüzyon,B. Subtilis,E. Coli

Kaynakça

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